Influence of Ginkgo Biloba extract (EGB 761) on expression of EGR-1 mRNA and HSP-70 mRNA after warm ischemia in the rat liver

Schutte, A. R.; Topp, Stefan A.; Knoefel, Wolfram Trudo; Brilloff, Silke; Müeller, Lars; Rogiers, Xavier; Gundlach, M.

doi:10.1016/s0041-1345(01)02520-9

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…One can speculate that the presence of EGb 761 reduces the cellular flux of free radicals, leading to a concomitant decrease in damaged proteins and a reduced requirement for HSP expression. This hypothesis was recently strengthened by reports that EGb 761 modulates HSP-70 expression in several model systems (37)(38)(39).…”

Section: Discussionmentioning

confidence: 91%

Expression of the small heat‐shock protein Hsp‐16‐2 inCaenorhabditis elegansis suppressed byGinkgo bilobaextract EGb 761

Strayer¹,

Wu²,

Christen

et al. 2003

FASEB j.

124

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EGb 761, a standardized extract of Ginkgo biloba leaves, has been shown to have antioxidative properties. We have previously demonstrated that EGb 761 increases stress resistance and mean life span in the model organism Caenorhabditis elegans. In this study, the molecular mechanism of EGb 761 on alleviating effects of oxidative stress is further investigated using transgenic C. elegans expressing a jellyfish green fluorescent protein (GFP)-tagged inducible small heat-shock protein gene (hsp-16-2). The expression of hsp-16-2 induced by the pro-oxidant juglone and by heat shock was significantly suppressed by 86% and 33%, respectively, in the transgenic nematode fed with EGb 761. These effects of EGb 761 correlate with its ability to increase mean survival rate of the nematode in response to acute oxidative and thermal stresses, as well as to attenuate the basal levels of hydrogen peroxide in the organism. Thus, we interpret the suppression of hsp-16-2/GFP expression as an indication that EGb 761 decreases cellular stress resulting from exogenous treatments, therefore leading to a decreased transcriptional induction of the reporter transgene. These results support the hypothesis that EGb 761 augments the natural antistress system of C. elegans, thus increasing stress resistance and life span.

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Section: Discussionmentioning

confidence: 91%

Expression of the small heat‐shock protein Hsp‐16‐2 inCaenorhabditis elegansis suppressed byGinkgo bilobaextract EGb 761

Strayer¹,

Wu²,

Christen

et al. 2003

FASEB j.

124

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“…In addition, rats that received EGb761 showed a lower increase in serum AST, ALT and LDH levels after hepatic IR when compared to rats in the control group. EGb761 might be a useful agent to attenuate postischaemic reperfusion injury, providing a variety of positive effects on hepatic microcirculation and liver regeneration [37].…”

Section: Discussionmentioning

confidence: 99%

Protective effects of N-acetylcysteine and Ginkgo biloba extract on ischaemia-reperfusion-induced hepatic DNA damage in rats

et al. 2008

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Hepatic ischaemia-reperfusion injury is a serious problem that occurs during various surgical operations such as liver transplantation, surgical revascularization, and partial organ resection. Different pharmacological agents have been used for the protection of organ function and for extending the tolerable ischaemic interval after the ischaemic insult. We aimed to determine the presence of 8-hydroxydeoxyguanosine (8-OHdG) in the DNA from liver undergoing ischaemia-reperfusion, and also to evaluate the protective effects of N-acetylcysteine (NAC) and EGb761 (Ginkgo biloba extract) against hepatic oxidative DNA damage. A total of 40 rats were divided into four groups of 10 animals each (sham-operation group, control group, NAC group, and EGb761 group). Oxidative damage to DNA was evaluated by measuring the increase in 8-OHdG formation in liver tissue and also the effects of NAC and EGb761 pretreatment. Hepatic ischaemia for 90 min followed by reperfusion caused a marked increase in tissue levels of 8-OHdG, thiobarbituric acid-reactive substance, serum ALT, AST and LDH activities compared to sham-operated group. Pretreatment with both NAC and EGb761 clearly diminished 8-OHdG formation and lipid peroxidation. These findings suggest that antioxidant molecules such as NAC and EGb761 may be useful in preventing postischaemic reperfusion injury in hepatic tissue.

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“…Many experimental studies have shown that Ginkgo biloba extract EGb 761 and other PAF antagonists can reduce or even prevent a possible injury in local and remote organ systems due to intestinal I-R [5][6][7][9][10][11][12]. As a Ginkgo biloba extract, EGb 761, prepared in a form suitable for experimental use, is a PAF antagonist with strong effects that scavenges toxic free radical activity [13]. While providing vascular relaxation, it prevents platelet aggregation and stabilizes lysosomal membranes.…”

Section: Introductionmentioning

confidence: 99%

“…This extract is used for therapeutic purposes in many ischemic events, including cerebrovascular and peripheral vascular insufficiency. EGb 761 has different effect potentials in different organs and systems and exhibits protective effects in neurodegenerative, sensory and vascular diseases [13,14]. This molecule, which can act systemically at molecular, cellular, and textural levels or in the whole organism, has no particular one-way (activator or inhibitory) effect; rather, it is a regulatory compound that helps the adaptation of an organism to the current environment [10,13,15].…”

Section: Introductionmentioning

confidence: 99%

The effect of Ginkgo biloba EGb 761 on intestinal anastomotic healing in rats with ischemia-reperfusion induced in the lower extremities

BASİM¹,

Basım²,

Demiray³

2021

Journal of Surgery and Medicine

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Background/Aim: Remote ischemia-reperfusion (I-R) injury for anastomotic healing is a newly identified risk factor and there is a wide array of studies being conducted. This study aimed to reveal the negative effects of lower I-R on the colonic anastomotic healing process and examine the effects of Ginkgo biloba EGb 761 treatment, a platelet activating factor (PAF) antagonist, on anastomotic healing through the inhibition of pathological mechanisms of mediators causing these negative effects. Methods: Thirty-six Wistar-Albino rats were divided into sham, I-R and I-R+EGb 761 groups, each consisting of 12 rats. In the subjects in the sham group, an end-to-end anastomosis was performed by transecting the descending colon following midline laparotomy. In the I-R group, unilateral lower extremity ischemia was created by occluding the femoral artery and collaterals using a tourniquet from the most proximal segment of the left extremity. Then, the descending colon was transected, anastomosis was performed, and reperfusion was created by a tourniquet application at the 30 th minute of ischemia. Different from the I-R (control) group, the subjects in the I-R+EGb 761 group were given two equal doses of 64 mg/kg/d Ginkgo biloba EGb 761 by the orogastric route until 10 days after surgery. After all subjects were sacrificed on the 10 th day of surgery, the descending colon segment containing the anastomosis area was resected and samples were taken for bursting pressure and hydroxyproline measurements. Results: In the I-R group, anastomotic bursting pressure and perianastomotic hydroxyproline values were significantly lower compared to the sham group and the I-R+EGb 761 group. However, there was no statistically significant difference in these parameters between the sham and the I-R+EGb 761 groups. Conclusion: Colonic anastomotic bursting pressure and peri-anastomotic hydroxyproline values in the sham group were significantly decreased by lower extremity I-R, and this change was prevented with the use of EGb 761.

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Influence of Ginkgo Biloba extract (EGB 761) on expression of EGR-1 mRNA and HSP-70 mRNA after warm ischemia in the rat liver

Cited by 5 publications

References 15 publications

Expression of the small heat‐shock protein Hsp‐16‐2 inCaenorhabditis elegansis suppressed byGinkgo bilobaextract EGb 761

Expression of the small heat‐shock protein Hsp‐16‐2 inCaenorhabditis elegansis suppressed byGinkgo bilobaextract EGb 761

Protective effects of N-acetylcysteine and Ginkgo biloba extract on ischaemia-reperfusion-induced hepatic DNA damage in rats

The effect of Ginkgo biloba EGb 761 on intestinal anastomotic healing in rats with ischemia-reperfusion induced in the lower extremities

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