“…Many functional groups including alkyne, alkene, ketone, and azides have been incorporated into the proteins via NAA, providing an orthogonal handle for protein modifications [122,223,224]. Halogenated NAAs, especially fluorinated amino acids, have made a significant impact on protein engineering [133,155,[225][226][227][228][229][230][231][232]. Repetitive protein polymers with fluorinated amino acid have shown distinct physicochemical properties, including an increase in thermal and chemical stability [155,228], as a result of the electronegativity and hydrophobicity of the fluorine atom [233,234].…”