Influence of Glutamine on Multiplication and Cytopathic Effect of Respiratory Syncytial Virus.

Márquez, A; Hsiung, G. D.

doi:10.3181/00379727-124-31674

Cited by 15 publications

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“…In addition, the two viruses lacking G protein, which usually show small syncytia in both medium 199 and OPTI-MEM, exhibit not only larger plaques but also much greater fusion in DMEM. The amounts of glutamine and calcium in the culture medium have been reported to affect syncytium formation (28,35). DMEM and medium 199 contain the same amount of calcium, but DMEM has twice the amount of glutamine.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Recombinant Respiratory Syncytial Virus Deletion Mutants Lacking the Small Hydrophobic and/or Attachment Glycoprotein Gene

Techaarpornkul

Barretto

Peeples

2001

J Virol

245

248

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Section: Discussionmentioning

confidence: 99%

Functional Analysis of Recombinant Respiratory Syncytial Virus Deletion Mutants Lacking the Small Hydrophobic and/or Attachment Glycoprotein Gene

Techaarpornkul

Barretto

Peeples

2001

J Virol

245

248

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“…It has been reported that glutamine plays a critical role in RSV-induced syncytium formation (8). In the absence of days postinfection FIG.…”

mentioning

confidence: 95%

Calcium requirement for syncytium formation in HEp-2 cells by respiratory syncytial virus

Shahrabadi

Lee

1988

J Clin Microbiol

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Respiratory syncytial virus (RSV) grown in HEp-2 cells in the absence of calcium did not induce cell fusion and syncytium formation. Although the infected cells contained viral antigens, the cytopathic effect (giant cell formation) typical for RSV was not observed in calcium-free cultures. Infectious virus yield was also slightly reduced (less than a one log10 reduction) in the absence of calcium. An analysis of viral proteins synthesized in both the presence and the absence of calcium revealed that the amount of fusion protein (F1) in calcium-free infected cultures was approximately one-third that in calcium-containing infected cultures. These results underscore the necessity of using calcium-containing growth medium for cell culture isolation and diagnosis of RSV.

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“…In calcium free medium the viral antigens are synthesized and accumulated in the cytoplasm of infected cells without cell fusion and syncytium formation (13). Glutamine have similar effect on cytopathogenicity of the virus (15). Two strains of RSV have been identified which cause respiratory disease (14) with no significant differences in pathogenicity.…”

Section: Discussionmentioning

confidence: 95%

Respiratory Syncytial Virus Infection in Children Referred to Kasra Hospital in Tehran during the Period of 2012-2014

2014

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Background and Aims: Respiratory syncytial virus (RSV) infection is common in infants and young children. In infants younger than one year old it may cause bronchiolitis and pneumonia which requires hospitalization. Accurate and rapid diagnosis of the disease will help proper treatment of the disease and prevent further complications. Materials and Methods: Specimen taken from respiratory tract of sick children were processed and fixed for immunofluorescence staining. Monoclonal antibody conjugate specific for RSV was used and the specimens were examined by a UV microscope. Results: In this study most of the infection occurred during the winter month such as Nov, Dec and Jan. From168 patients, 111 referred from Dec to Feb and 51% of them were positive. Most of the patient were under one year and the majority were up to 8 month old. However there was one patient, a 36 old woman which was positive indicating that RSV infection can occur in adults. There were no significant changes in infection between the males and females. Conclusion:The results of this study showed that disease is common during the winter time in children in Tehran and a rapid reliable test such as immunofluorescence should be applied for prompt detection of the disease and its proper treatment.

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Influence of Glutamine on Multiplication and Cytopathic Effect of Respiratory Syncytial Virus.

Cited by 15 publications

References 0 publications

Functional Analysis of Recombinant Respiratory Syncytial Virus Deletion Mutants Lacking the Small Hydrophobic and/or Attachment Glycoprotein Gene

Functional Analysis of Recombinant Respiratory Syncytial Virus Deletion Mutants Lacking the Small Hydrophobic and/or Attachment Glycoprotein Gene

Calcium requirement for syncytium formation in HEp-2 cells by respiratory syncytial virus

Respiratory Syncytial Virus Infection in Children Referred to Kasra Hospital in Tehran during the Period of 2012-2014

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