Influence of Glutathione on Kinetic Parameters of Frozen-Thawed Spermatozoa from Ovchepolian Pramenka Rams

Nikolovski, Martin; Mickov, Ljupco; Dovenska, Monika; Petkov, V.; Atanasov, B; Dovenski, Toni

doi:10.14432/j.macvetrev.2014.05.014

Cited by 6 publications

(4 citation statements)

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“…CASA methods allow the possibility of real-time analysis of spermatozoon kinetic parameters, providing fast and accurate results. They have good predictive value for fertilization ability (Nikolovski et al, 2014). Robayo et al (2008) reported that the progression of spermatozoa in sheep cervical mucus was positively correlated with the kinetic parameter VCL.…”

Section: Discussionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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The aim of this study was to determine the effects of thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of ram semen during cryopreservation. Ejaculates were collected from five Sonmez rams using an artificial vagina and extended with Tris‐based extender not containing TQ (control, 0 μg/ml TQ) and containing 10, 25, 50 and 100 μg/ml TQ. The extended semen samples were equilibrated in a + 4°C cold cabinet for 2 h. After 2 h, the samples were loaded into 0.25 ml French straws. The straws were frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (−196°C). The frozen straws were thawed in a water bath (37°C for 30 s) and evaluated in terms of motility characteristics, plasma membrane and acrosome integrity, mitochondrial reactive oxygen species levels, lipid peroxidation levels, DNA damage and biochemical alterations (oxidative stress index, malondialdehyde and glutathione). TQ100 had higher total motility (53.59 ± 3.01) and progressive motility (19.84 ± 1.44; not significantly different from TQ25 and TQ50) compared to the control and TQ10 (p ˂ 0.05). According to the results of the analyses on motility characteristics, there were significant differences between the groups in terms of curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; p ˂ 0.05). The highest DNA damage was detected in the control group (p ˂ 0.05). TQ50 had higher plasma membrane and acrosome integrity (59.56 ± 5.92) compared to the control and TQ25 (p < 0.05) but not significantly different from TQ10 and TQ100. The lowest mitochondrial reactive oxygen species levels were detected in TQ50 and TQ100 (p ˂ 0.05). There were no significant differences among the groups in terms of their oxidative stress index, lipid peroxidation, malondialdehyde and glutathione levels (p > 0.05). According to the results, it could be concluded that supplementing 50 or 100 μg/ml TQ to Tris extenders that were used for ram semen cryopreservation showed a positive effect on motility, plasma membrane integrity and acrosome integrity, and it reduced DNA damage and mitochondrial reactive oxygen species levels.

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Section: Discussionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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“…Numerous researchers have been focusing on the effects of different antioxidants supplemented to sperm cryopreserving media (oxidized glutathione-GSSG, reduced glutathione-GSH, cysteine) in aim to reduce the vii oxidative stress when supplemented in ram semenfreezing media. Most of these studies reported that these antioxidants have no effect in increasing ram sperm viability during cryopreservation (40,41).…”

Section: Semen Conservation/preservationmentioning

confidence: 99%

Novelties in Ovine Assisted Reproductive Technologies – A Review

Dovenski

Trojačanec

Atanasov

et al. 2022

Macedonian Veterinary Review

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Artificial insemination (AI) as a part of assisted reproductive technologies represents the oldest and most widespread method used to accelerate genetic progress in all domestic animals. After its first implementation in ovine reproduction and almost 80 years afterward, AI is continuously used for improving the genetic merit, utilizing either fresh or short-time chilled semen. Nevertheless, regardless of the semen used for insemination, the conception rate (CR) is still lower in comparison to natural service. At least two factors are commonly thought to limit the success of the AI and reduce the CR: (1) failure of placing the semen directly into the uterus due to the specific anatomic structure of the ewe’s cervix; (2) lower viability of ram spermatozoa during cryopreservation (<30% progressively motile spermatozoa after thawing). This review elaborates on recent studies that aimed to achieve acceptable CR through the implementation of cervical or intrauterine insemination: deep intracervical, intrauterine trans-cervical, and intracornual. Several hormonal treatments (oxytocin, estrogen, or prostaglandin) were evaluated on inducing cervical dilation that facilitates insemination. A comprehensive analysis was given to the effects of several antioxidants (GSSG, GSH, and cysteine) supplemented in ram semen-freezing media. Sex-sorted ram semen fertility rate results were presented from our studies.

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“…Using antioxidants as a protective agent against the formation of free radicals is known scientifically as a strong tool in decreasing oxidative stress and maintaining the biological activities of cells. The use of antioxidants has a confirmed positive action on enhancing the fertile properties of semen because they scavenge free radicals that form during the metabolic activities of sperm and leukocytes, they decrease the development of premature sperm, and they prevent DNA fragmentation of sperm (Nikolovski et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Alpha-lipoic acid protects against hyperlipidemia-induced testicular damage in premature rats

Alsaad,

Al-Kufaishi,

Karam

2023

Multidiscip. Sci. J.

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This study was designed to investigate the effect of hypercholesterolemia on the reproductive performance of premature male rats and to evaluate the influence of alpha lipoic acid (ALA) in conserving their fecundity. Sixty rats were randomly assigned to one of three groups. The control group (CG n=20 rats), cholesterol feeding group 1 (CFG1 n=20 rats) were fed 1.5% cholesterol with diet for one month, and cholesterol feeding group 2 (CFG2 n= 20 rats) were fed 1.5% cholesterol with diet + ALA 100 mg/kg body weight supplied by water for one month. The results revealed that compared to CG and CFG2, CFG1's body weight increased significantly. After consuming cholesterol for one month, CFG1's lipid profile revealed a substantial rise in blood cholesterol and triglycerides when compared to CG and the group that consumed ALA CFG2. In comparison to CGF1 and CG, the results of the sperm parameters in CGF2 demonstrated a large rise in sperm count with sperm live percentage and a considerable decrease in sperm abnormality %. Rats from CFG1 had significantly lower serum testosterone levels than rats from CFG2 and CG, according to the hormonal profile. We conclude that the significance of alpha lipoic acid (ALA) in preserving fertility ALA is a strong antioxidant that may preserve the parameters of the sperm of hyperlipidemic preterm rats, which may improve the capacity of hyperlipidemic rats to conceive.

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Influence of Glutathione on Kinetic Parameters of Frozen-Thawed Spermatozoa from Ovchepolian Pramenka Rams

Cited by 6 publications

References 50 publications

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Novelties in Ovine Assisted Reproductive Technologies – A Review

Alpha-lipoic acid protects against hyperlipidemia-induced testicular damage in premature rats

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