Influence of growth conditions on hydrophobicity of<i>Lactobacillus acidophilus</i>and<i>Bifidobacterium lactis</i>cells and characteristics by FT-IR spectra

Shakirova, L. Sh.; Auzina, Lilija; Zikmanis, Pēteris; Gavare, Marita; Grūbe, Māra

doi:10.1155/2010/659167

Cited by 16 publications

(16 citation statements)

References 16 publications

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“…Polysaccharides are believed to contrast cell aggregation and adhesion 23 , suggesting that a decrease of these compounds could be a strategy of B. breve DSM 20213 to improve the hydrophobicity and auto-aggregation of its cells when exposed to LA. Previously, Shakirova et al 24 reported an increase of cell surface hydrophobicity in relation to decreased carbohydrate levels in Bifidobacterium lactis Bb12.…”

Section: Resultsmentioning

confidence: 98%

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213

Senizza

Rocchetti

Callegari

et al. 2020

Sci Rep

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Despite clinical and research interest in the health implications of the conjugation of linoleic acid (LA) by bifidobacteria, the detailed metabolic pathway and physiological reasons underlying the process remain unclear. This research aimed to investigate, at the molecular level, how LA affects the metabolism of Bifidobacterium breve DSM 20213 as a model for the well-known LA conjugation phenotype of this species. the mechanisms involved and the meaning of the metabolic changes caused by LA to B. breve DSM 20213 are unclear due to the lack of comprehensive information regarding the responses of B. breve DSM 20213 under different environmental conditions. Therefore, for the first time, an untargeted metabolomics-based approach was used to depict the main changes in the metabolic profiles of B. breve DSM 20213. Both supervised and unsupervised statistical methods applied to the untargeted metabolomic data allowed confirming the metabolic changes of B. breve DSM 20213 when exposed to LA. In particular, alterations to the amino-acid, carbohydrate and fatty-acid biosynthetic pathways were observed at the stationary phase of growth curve. Among others, significant upregulation trends were detected for aromatic (such as tyrosine and tryptophan) and sulfur amino acids (i.e., methionine and cysteine). Besides confirming the conjugation of LA, metabolomics suggested a metabolic reprogramming during the whole growth curve and an imbalance in redox status following LA exposure. Such redox stress resulted in the down-accumulation of peroxide scavengers such as lowmolecular-weight thiols (glutathione-and mycothiol-related compounds) and ascorbate precursors, together with the up-accumulation of oxidized (hydroxy-and epoxy-derivatives) forms of fatty acids. Consistently, growth was reduced and the levels of the oxidative stress marker malondialdehyde were higher in LA-exposed B. breve DSM 20213 than in the control.

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Section: Resultsmentioning

confidence: 98%

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213

Senizza

Rocchetti

Callegari

et al. 2020

Sci Rep

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“…An increase in the growth of B. longum during the lag phase and some morphological modifications as well as changes in the fatty acid profile have already been reported (Ahn et al, 2001), whereas, for B. animalis ssp. lactis Bb12, changes in the surface hydrophobicity and an increase in protein content in the presence of increased oxygen concentration was observed by Shakirova et al (2010) Other factors to be considered are that aerobic conditions decrease the production of exopolysaccharides in some bifidobacteria strains, such as B. longum (Ninomiya et al, 2009), and this can be considered negative, as exopolysaccharides are able to increase viscosity and confer a desirable texture to the fermented product (Salazar et al, 2009).…”

Section: Postacidification Oxygen Levels and Proteolysismentioning

confidence: 99%

Probiotic yogurts manufactured with increased glucose oxidase levels: Postacidification, proteolytic patterns, survival of probiotic microorganisms, production of organic acid and aroma compounds

Cruz

Castro

Faria

et al. 2012

Journal of Dairy Science

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We investigated the effect of increased glucose oxidase concentration as a technological option to decrease oxidative stress during the processing of probiotic yogurts. Probiotic yogurts were produced with increased concentrations of glucose oxidase (0, 250, 500, 750, or 1,000 mg/kg) and submitted to physicochemical and microbiological analysis at 1, 15, and 30 d of refrigerated storage. Higher concentrations of glucose oxidase (750 and 1,000 mg/kg) and a longer storage time were found to have an influence on the characteristics of the probiotic yogurt, contributing to more extensive postacidification, an increase in the dissolved oxygen level, and higher proteolysis. In addition, increased production of aroma compounds (diacetyl and acetaldehyde) and organic acids (mainly lactic acid) and a decrease in the probiotic bacteria count were reported. The use of glucose oxidase was a feasible option to minimize oxidative stress in probiotic yogurts. However, supplementation with excessive amounts of the enzyme may be ineffective, because insufficient substrate (glucose) is present for its action. Consumer tests should be performed to evaluate changes in the sensory attributes of the probiotic yogurts with increased supplementation of glucose oxidase. In addition, packaging systems with different permeability to oxygen should be evaluated.

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“…The three strains of Lactobacillus (i.e., L. acidophilus , L. gasseri , and L. jensenii ) used by Boris et al [ 67 ] showed about 80% surface hydrophobicity. B. lactis Bb12 and L. acidophilus LA5 showed surface hydrophobicity with values between 61% and 75% [ 68 ]. B. pseudolongum CIDCA 531 expressed 85% surface hydrophobicity [ 69 ].…”

Section: Cell Adhesive Propertymentioning

confidence: 99%

Review on Bifidobacterium bifidum BGN4: Functionality and Nutraceutical Applications as a Probiotic Microorganism

Park

et al. 2016

IJMS

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Bifidobacterium bifidum BGN4 is a probiotic strain that has been used as a major ingredient to produce nutraceutical products and as a dairy starter since 2000. The various bio-functional effects and potential for industrial application of B. bifidum BGN4 has been characterized and proven by in vitro (i.e., phytochemical bio-catalysis, cell adhesion and anti-carcinogenic effects on cell lines, and immunomodulatory effects on immune cells), in vivo (i.e., suppressed allergic responses in mouse model and anti-inflammatory bowel disease), and clinical studies (eczema in infants and adults with irritable bowel syndrome). Recently, the investigation of the genome sequencing was finished and this data potentially clarifies the biochemical characteristics of B. bifidum BGN4 that possibly illustrate its nutraceutical functionality. However, further systematic research should be continued to gain insight for academic and industrial applications so that the use of B. bifidum BGN4 could be expanded to result in greater benefit. This review deals with multiple studies on B. bifidum BGN4 to offer a greater understanding as a probiotic microorganism available in functional food ingredients. In particular, this work considers the potential for commercial application, physiological characterization and exploitation of B. bifidum BGN4 as a whole.

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Influence of growth conditions on hydrophobicity ofLactobacillus acidophilusandBifidobacterium lactiscells and characteristics by FT-IR spectra

Cited by 16 publications

References 16 publications

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213

Probiotic yogurts manufactured with increased glucose oxidase levels: Postacidification, proteolytic patterns, survival of probiotic microorganisms, production of organic acid and aroma compounds

Review on Bifidobacterium bifidum BGN4: Functionality and Nutraceutical Applications as a Probiotic Microorganism

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