Influence of growth regulators and light on<i>in vitro</i>shoot regeneration in M.26 apple roostock

Ferradini, Nicoletta; Famiani, Franco; Proietti, Primo; Stănică, F.

doi:10.1080/14620316.1996.11515468

Cited by 14 publications

(9 citation statements)

References 3 publications

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“…Similarly, a high BA concentration (22.2 lM) in regeneration media produced a higher percentage of regenerating leaves in 'M26' rootstock compared to those cultured on media with 11.1 lM BA, but the number and length of shoots were not affected (Famiani et al 1994). A further increase in BA concentration (to 33.3 and 44.4 lM) significantly decreased the regeneration parameters of 'M26' rootstock (Ferradini et al 1996). A higher concentration of BA (31.1 lM) proved to be optimal for 'Golden Delicious' and 'Empire' (Yepes and Aldwinckle 1994).…”

Section: Cytokinins During the Regeneration Processmentioning

confidence: 78%

“…The highest value was obtained with cytokinin-free media and leaves excised 30 days after the beginning of subculture, but the number of shoots was not affected by pre-treatments. In experiments of Ferradini et al (1996), the number of shoots per explant was also higher when source shoots were collected from media without cytokinin. In both cases, the cytokinin-free pretreatment was compared to the pre-treatment with BA.…”

Section: Cytokinin and In Vitro Shoot Regeneration Of Applementioning

confidence: 92%

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The role of cytokinins in shoot organogenesis in apple

Magyar-Tábori

Dobránszki

Silva

et al. 2010

Plant Cell Tiss Organ Cult

133

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Effective regeneration in vitro is a necessary precondition for the implementation of different biotechnological approaches in plant breeding. Numerous studies have reported on regeneration from apple somatic tissues, and organogenesis has been proved to be influenced by several factors including mother shoots (genotype, size, type, and age of explant), in vitro conditions (dark period, light intensity, and quality), and others (wounding, orientation of leaf explants). However, one of the most important factors before and during the regeneration process is the type and concentration of cytokinin applied. Thidiazuron and benzyladenine are the most frequently used cytokinins in the regeneration systems, but their efficiency depends on genotype and other factors. Other cytokinins (e.g., zeatin and kinetin) have also been tested in several experiments and they were found in general to be less active. The organogenic ability of explants can also be increased by a properly selected cytokinin pre-treatment. Cytokinins applied in the pre-treatments can influence the leaf structure, which in turn can alter the regeneration capacity of the leaf explant. Interactions between factors of pre-treatments (hormones, light, and culture conditions) and factors of the regeneration phase should be considered. This review brings into focus the role of different cytokinins during in vitro shoot development, discussing their effects on the histology of leaves developed in vitro, and how this affects the subsequent regeneration process.

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Section: Cytokinins During the Regeneration Processmentioning

confidence: 78%

Section: Cytokinin and In Vitro Shoot Regeneration Of Applementioning

confidence: 92%

The role of cytokinins in shoot organogenesis in apple

Magyar-Tábori

Dobránszki

Silva

et al. 2010

Plant Cell Tiss Organ Cult

133

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“…Para a cultura in vitro da macieira, a organogênese depende da origem do explante (Collet & Lê, 1987), podendo ser oriundo de pecíolo, lâmina foliar, entre outros ( Arena & Pastur, 1995). Ferradini et al (1996) utilizaram a superfície abaxial de folhas escarificadas, em contato com o meio de cultura, e obtiveram bons resultados de calogênese com uso de BAP. Nieuwkerk et al (1986) relatam o uso de forchlorfenuron (CPPU) na multiplicação de brotos de macieira, em concentrações bem inferiores ao BAP, sendo que Liu et al (1994), cultivando calos de macieira cv.…”

Section: Index Terms: In Vitro Culture Tissue Explantunclassified

“…Ferradini et al (1996) utilizaram o mesmo tipo de explantes e obtiveram bons resultados na calogênese. Segundo Bartish & Korkoroi (1997), utilizando a superfície abaxial em contato com o meio, foi mais efetiva na formação de brotos .…”

Section: Index Terms: In Vitro Culture Tissue Explantunclassified

CALOGÊNESE DE TECIDO FOLIAR DE PORTA-ENXERTO DE MACIEIRA M.7 (MALUS sp.) INDUZIDA POR BAP E CPPU

Martins¹,

Sczepanski²,

Bachettini³

et al. 2001

Rev. Bras. Frutic.

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O trabalho foi conduzido com o objetivo de estudar a organogênese de macieira (Malus sp), após a obtenção de calo por meio de explantes de folhas do porta-enxerto M.7 multiplicado in vitro. O experimento foi conduzido no Laboratório de Cultura de Tecidos da Embrapa Clima Temperado, utilizando folhas com a superfície abaxial e adaxial em contato com o meio, com ou sem escarificação; associados às citocininas Benzilaminopurina (BAP) e Forchlorfenuron (CPPU) na concentração de 5mM. Utilizou-se o meio básico MS acrescido de sacarose (30 g.L-1 ), mio-inositol (100 mg.L-1) e ágar (6 g.L-1), além do regulador de crescimento Ácido Naftalenoácetico (ANA 0,5mM). Os tratamentos permaneceram por três semanas no escuro sob temperatura ambiente, o que propiciou 100% de formação de calos, sendo em seguida submetidos a fotoperíodo de 16 horas com intensidade luminosa de 20 mE.m-2.s-1 e temperatura de 25 ± 2ºC. Os explantes escarificados proporcionaram maior intensidade de calo do que a utilização de explantes intatos. Explantes escarificados com a superfície abaxial em contato com o meio proporcionaram maior intensidade de calo, independentemente de o meio conter BAP ou CPPU. O uso da escarificação, associado ao CPPU, promoveu uma maior intensidade de calo, independentemente da superfície do disco foliar. A superior regeneração de calos foi alcançada em condição de superfície abaxial do disco foliar associado ao CPPU. Portanto, o uso de explantes escarificados com a superfície abaxial em contato com o meio proporcionou aumento da intensidade de calo. O uso do explante escarificado em meio contendo CPPU proporcionou maior intensidade de calo, independentemente da superfície do disco foliar em contato com o meio.

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“…Darkness allows leaf explants to express their organogenetic potential in the subsequent regeneration phase, resulting in more shoots (Famiani et al 1994). Darkness probably keeps endogenous auxin at high levels as auxin is light sensitive (Ferradini et al 1996).…”

Section: Effects Of Dark Treatment On Regenerationmentioning

confidence: 99%

Regeneration from leaf segments ofinvitro‐grown shoots ofMalus baccata

Sun

Wang

et al. 2008

New Zealand Journal of Crop and Horticultural Science

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Adventitious shoot regeneration was investigated using leaf explants of in vitro cultured shoots of Malus baccata. Optimal regeneration was obtained using Murashige and Skoog medium supplemented with 4 mg/litre 6-benzyladenine and 0.5 mg/litre α-naphthalene-acetic acid. Dark incubation for 10 days gave the best results of adventitious shoot regeneration. Sorbitol was proved to be the most suitable carbon source for shoot regeneration in M. baccata. gelling with either Phytagel or gelrite was more efficient than agar in shoot regeneration. The highest regeneration frequency was 97% and the greatest shoot number per regenerated leaf explant was 8.7. The addition of antibiotics Kanamycin or Hygromycin significantly reduced regeneration capacity in most instances. The development of the regeneration protocol in this study will facilitate the future transformation work in this species.

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Influence of growth regulators and light onin vitroshoot regeneration in M.26 apple roostock

Cited by 14 publications

References 3 publications

The role of cytokinins in shoot organogenesis in apple

The role of cytokinins in shoot organogenesis in apple

CALOGÊNESE DE TECIDO FOLIAR DE PORTA-ENXERTO DE MACIEIRA M.7 (MALUS sp.) INDUZIDA POR BAP E CPPU

Regeneration from leaf segments ofinvitro‐grown shoots ofMalus baccata

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