Influence of Growth Temperature on Respiratory Characteristics of Mitochondria from Callus-Forming Potato Tuber Discs

Abstract

The uninhibited respiration of mitochondria, isolated from potato tuber discs (Solamem tuberosum L. cv A positive correlation has been demonstrated between the incubation temperature (ranging from 4-37°C) and the relative capacity of respiration via alternative pathway in the mitochondria. Induction of alternative pathway is not directly correlated with growth (in terms of increase in fresh weight) of the potato tuber discs.

Effects of Copper on Total Phenolics, Flavonoids and Mitochondrial Properties of Orthosiphon stamineus Callus Culture

“…Previously, the streptomycin resistance of tobacco cultured cells and the response of mitochondria from calluses-forming potato tuber disks towards ethanol and growth temperature were reported (Plas and Wagner, 1980;Hemrika-Wagner et al, 1982;Zamski and Umiel, 1982). Quite recently, the effects of nitric oxidase to mitochondrial respiration of Arabidopsis thaliana calluses were investigated (Wang et al, 2010).…”

Adenine nucleotide contents in callus‐forming potato tuber discs with different respiratory characteristics

“…Respiration of potato tuber callus consists of two components: a CN^-sensitive SHAM-resistant part via the cytochrome pathway and a CN~-resistant SHAM-sensitive part via the alternative pathway. There is a positive correlation between the growth temperature of these discs atid the capacity of respiration via the alternative pathway (Hemrika-Wagner et al 1982, 1983. The participation of the alternative pathway in uninhibited respiration, however, was relatively high during growth at low temperatures (tbe ratio of respiration along the cytochrome pathway and the altemative pathway was 3.5:1 during growth at 28°C and 2.,3:1 during growth at 8°C, Tab.…”

“…Electron transport via both pathways can iead to ATP synthesis,, but ATP synthesis related to the operation of the cytochrome pathway is higher than the synthesis related to the operation of the alternative pathway (ADP/ O ratio is 2-3 for the cytochrottie pathway and 0-1 for the alternative pathway, depending on the substrate (Laties 1982). Apart from these differences in "efficiency" (the amount of ATP produced per molecule of Q-sugar degraded), respiratioti rates of callus growing at 8°C were half those of callus growing at 28°C (Hemrika-Wagner et al 1983), which in itself would have resulted in a lower production of ATP at 8°C.…”

“…The participation of the alternative pathway in uninhibited respiration, however, was relatively high during growth at low temperatures (tbe ratio of respiration along the cytochrome pathway and the altemative pathway was 3.5:1 during growth at 28°C and 2.,3:1 during growth at 8°C, Tab. 1 in Hemrika-Wagner et al 1983). Participation of the alternative pathway was low at the start of callus induction, reaching its maximtim during the exponential growth phase (Hemrika-Wagner et al 1983).…”

“…1 in Hemrika-Wagner et al 1983). Participation of the alternative pathway was low at the start of callus induction, reaching its maximtim during the exponential growth phase (Hemrika-Wagner et al 1983). Electron transport via both pathways can iead to ATP synthesis,, but ATP synthesis related to the operation of the cytochrome pathway is higher than the synthesis related to the operation of the alternative pathway (ADP/ O ratio is 2-3 for the cytochrottie pathway and 0-1 for the alternative pathway, depending on the substrate (Laties 1982).…”