Influence of GSTP1 gene polymorhism on decreased semen quality

Abstract

Background ― Genetic polymorphisms analysis of metabolic and antioxidant systems pathway genes are associated with male infertility is the most perspective and developed field in andrology. Purpose ― the aim of the research is to reveal the association of the glutathione S-transferase gene GSTP1 polymorphism C/T (rs1138272) with risk of pathospermia in Russian men in Moscow region. Material and Methods ― Case control study was conducted on 68 fertile men and 70 infertile men with various forms of pathospermia. Sperm analysis was performed according to WHO guidelines (WHO, 2010). DNA was extracted from peripheral blood leukocytes. Genotyping of the GSTP1 gene polymorphisms was carried out by generated amplicons from melting curve analysis after real time PCR. Results ― statistically significant association of polymorphism GSTP1 C/T (Ala/Val; rs1138272) with asthenozoospermia (χ2=8.58, p=0.003) and teratospermia (χ2=6.81, p=0.009) risk was found. The frequencies of homozygous and heterozygous carries (CT+TT genotypes) for polymorphic locus GSTP1 gene (rs1138272) are 3 times higher for men with disturbance of motility of spermatozoa and 2.5 times higher for men with abnormalities in morphology of spermatozoa, then for men with normozoospermia. Conclusion ― The GSTP1 C>T polymorphism (rs1138272) associated with risk of teratospermia and asthenozoospermia in male of reproductive age.

Association of DNA fragmentation of spermatozoa and the success of assisted reproductive technology programs

“…We found a direct correlation between reproductive losses (spontaneous abortion in early pregnancy) and the level of DNA fragmentation (Fig. 1) tozoa in male infertility [6,10]. Palermo Gianpiero D. et al showed an inverse correlation between DNA fragmentation and sperm mobility [11].…”

Parameters of free radical oxidation and antioxidant defense in patients with cervical cancer before and after radical surgical treatment

“…Известно, что основная протективная роль при воздействии окислительного стресса принадлежит неферментативному звену антиоксидантов (глутатион, витамины А, Е), так и ферментативным антиоксидантам (супероксиддисмутаза (СОД), глутатионпероксидаза, каталаза) [23]. Результаты функционирования неферментативных и ферментативных антиоксидантных систем играют важную роль в регуляции канцерогенеза, в том числе, в процессах, стимулирующих прогрессию опухоли, а также формирование ее резистентности к проводимой терапии [14].…”

Study of the activity of the antioxidant system in experimental periodontitis

“…Due to chronic inflammation in the periodontal tissues, neutrophils and lymphocytes are activated enabling synthesis of cytokines and prostaglandins [2]. Long-term chronic inflammation in tissues leads to an increase in reactive oxygen species (ROS), and an imbalance in the antioxidant system [3,4]. The long-term chronic inflammation is known to facilitate destruction of tissues supporting teeth, including the gums, periodontal ligament, and alveolar bone [5].…”