Influence of GSTT1 and GSTM1 Genotypes on Sunburn Sensitivity

Abstract

Individuals harboring the *0/*0 genotype of GSTT1 and/or GSTM1 showed enhanced UVB-induced cutaneous damage. Moreover, GST genotypes modulated Hypericum-induced photosensitization.

Regulation of cutaneous drug-metabolizing enzymes and cytoprotective gene expression by topical drugs in human skin in vivo

“…This may be a factor which contributes to our observed variation in cutaneous gene expression as, for example, inheritance of specific allelic variants of certain genes, e.g. GSTM1 and GSTT1, has previously been associated with responses to UVR and susceptibility to skin cancer 32,33 . However, for the majority of the genes studied here, e.g.…”

Induction of the Phase 2 Response in Mouse and Human Skin by Sulforaphane-containing Broccoli Sprout Extracts

“…Similarly, NQO1-deficient mice are much more sensitive to skin carcinogenesis compared with their wild-type counterparts (18). In humans, an inverse association between NQO1-positive genotype and the number of cutaneous basal cell carcinomas has been observed (19), as well as higher sensitivity to UV-radiation-induced cutaneous damage in individuals homozygously deficient in GSTT1 and/or GSTM1 (20). Thus, both human polymorphisms of phase 2 enzymes and deletion of their genes in mice increase susceptibility to skin tumor development.…”

High-Throughput Detection of Glutathione S -Transferase Polymorphic Alleles in a Pediatric Cancer Population

“…Neither the high-throughput assay nor the SNE assays was able to distinguish GSTM1*A or GSTM1*B homozygotes (i.e., GSTM1*A/A and GSTM1*B/B) from corresponding heterozygotes, GSTM1*A/null and GSTM1*B/null, respectively. Therefore, to determine gene dosage for GSTM1, a long-range PCR to confirm or negate the presence of the GSTM1*0 allele was developed by modification of a previously reported protocol (31). Using the Expanded Long Template PCR System (Roche, Mannheim, Germany) and primers M2F10 (5Ј-AAGA-CAGAGGAAGGGTGCATTTGATA-3Ј) and M5R16A (5Ј-ACAGACATTCATTCCCAAAGCGAC-3Ј), a 4.8-kb product can be amplified in the presence of a GSTM1*0 allele.…”

“…GSTT1 Long-Range PCR Assay for Determination of Gene Dosage. To determine gene dosage for GSTT1, a long-range PCR was developed by modification of a previously reported protocol (31). Two sets of primers were used, one specific for the GSTT1*0 allele and the other nonspecific for the GSTT1/ GSTT2 alleles.…”