Influence of H2TOEtPyP4 porphyrin on the stability and conductivity of bilayer lipid membranes

Torosyan, Anahit; Arakelyan, V.B.

doi:10.1007/s00249-015-1074-1

Cited by 4 publications

(6 citation statements)

References 17 publications

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“…Studies have reported that physicochemical properties of bilayer lipid membranes are altered after binding with PpIX [37]. The modified membranes become more vulnerable and hypersensitive to ultrasound [11], and more easily to form pores in the presence of an electrical field [38]. Therefore, the exact mechanisms of MP-SDT are complex and still need further investigation.…”

Section: Discussionmentioning

confidence: 99%

Membrane-permeabilized sonodynamic therapy enhances drug delivery into macrophages

et al. 2019

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Macrophages play a pivotal role in the formation and development of atherosclerosis as a predominant inflammatory cell type present within atherosclerotic plaque. Promoting anti-atherosclerotic drug delivery into macrophages may provide a therapeutic potential on atherosclerotic plaque. In this study, we investigated whether membrane-permeabilized sonodynamic therapy (MP-SDT) enhances drug delivery into THP-1 macrophages. Images of confocal microscopy confirmed that the optimal plasma distribution of the sonosensitizer protoporphyrin IX (PpIX) was at 1 hour incubation. The non-lethal parameter of MP-SDT was determined by cell viability as measured by a CCK-8 assay. Bright field microscopy demonstrated plasma membrane deformation in response to MP-SDT. Using SYTOX Green, a model drug for cellular uptake, we found that MP-SDT significantly induced membrane permeabilization dependent on ultrasound intensity and exposure time. Using Fluo-3 AM, intracellular calcium elevation during MP-SDT was confirmed as a result of membrane permeabilization. Membrane perforation of MP-SDT-treated cells was observed by scanning electron microscopy and transmission electron microscopy. Moreover, MP-SDT-induced membrane permeabilization and perforation were remarkably prevented by scavenging reactive oxygen species (ROS) during MP-SDT. Furthermore, we assessed the therapeutic effect of MP-SDT in combination with anti-atherosclerotic drug atorvastatin. Our results showed that MP-SDT increased the therapeutic effect of atorvastatin on lipid-laden THP-1-derived foam cells, including decreasing lipid droplets, increasing the cholesterol efflux and the expression of PPARγ and ABCG1. In conclusion, MP-SDT might become a promising approach to facilitating the delivery of anti-atherosclerotic drugs into macrophages via membrane permeabilization.

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Section: Discussionmentioning

confidence: 99%

Membrane-permeabilized sonodynamic therapy enhances drug delivery into macrophages

et al. 2019

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“…The stability of the lipid bilayer can be decreased by interaction with cationic porphyrins [26] which can be beneficial to drug absorption. Porphyrin-BSA complexes also have higher stability [28].…”

Section: Discussionmentioning

confidence: 99%

“…Cationic porphyrins can interact with lipid bilayers and reduce their stability [26]. Quadraplexes can be stabilized by porphyrins with a high number of charges [27].…”

Section: Introductionmentioning

confidence: 99%

Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

Wang

Liang

et al. 2019

IJMS

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Type 2 diabetes mellitus (T2DM) is associated with pancreatic β-cell dysfunction which can be induced by oxidative stress. Deuterohemin-βAla-His-Thr-Val-Glu-Lys (DhHP-6) is a microperoxidase mimetic that can scavenge reactive oxygen species (ROS) in vivo. In our previous studies, we demonstrated an increased stability of linear peptides upon their covalent attachment to porphyrins. In this study, we assessed the utility of DhHP-6 as an oral anti-diabetic drug in vitro and in vivo. DhHP-6 showed high resistance to proteolytic degradation in vitro and in vivo. The degraded DhHP-6 product in gastrointestinal (GI) fluid retained the enzymatic activity of DhHP-6, but displayed a higher permeability coefficient. DhHP-6 protected against the cell damage induced by H2O2 and promoted insulin secretion in INS-1 cells. In the T2DM model, DhHP-6 reduced blood glucose levels and facilitated the recovery of blood lipid disorders. DhHP-6 also mitigated both insulin resistance and glucose tolerance. Most importantly, DhHP-6 promoted the recovery of damaged pancreas islets. These findings suggest that DhHP-6 in physiological environments has high stability against enzymatic degradation and maintains enzymatic activity. As DhHP-6 lowered the fasting blood glucose levels of T2DM mice, it thus represents a promising candidate for oral administration and clinical therapy.

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“…The stability of the BLM in an electric field is defined as the average lifetime of the BLM at a given voltage. In [9][10][11], the BLM stability in an electric field was experimentally studied and its theoretical description (the theory of BLM electrical breakdown) was developed in detail. These studies made it possible to reveal the main parameters on which the stability of the BLM in an electric field depends and to determine them by comparing the experimental data with the theoretical curve of the dependence of the average BLM lifetime t (ϕ) on the potential ϕ.…”

mentioning

confidence: 99%

“…The experiment and procedure for comparing the experimental data with the theoretical curve (2) were thoroughly described in [11]. Experimental points, which depicted the dependence of the average BLM lifetime on the potential, were obtained at first in the absence of nanoparticles in the solution, and then in the presence of aluminum oxide nanoparticles in different concentrations.…”

mentioning

confidence: 99%

STABILITY AND CONDUCTIVITY OF BILAYER LIPID MEMBRANE IN PRESENCE $\mathrm{Al_2O_3}$ NANOPARTICLES

Jomardyan,

Ananyan,

Arakelyan

2023

Proc. YSU A: Phys. Math. Sci.

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The effect of aluminum oxide nanoparticles (Al2O3) on the stability and conductivity of BLM (the bilayer lipid membrane) in solution was studied. It has been shown that Al2O3 nanoparticles increase the stability of BLM in an electric field, and BLM becomes more stable with increasing their concentration. The experimental data are analyzed in terms of the well-known theory of BLM stability, which is based on the concept of spontaneous formation of microscopic pores on the BLM, the development of which leads to the loss of BLM stability. It is shown that aluminum oxide nanoparticles increase the value of the coefficient of linear tension of the pore edge, and with an increase in the concentration of nanoparticles, the linear tension also increases. It has been shown that the presence of nanoparticles in the solution leads to a decrease in BLM conductivity.

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Influence of H2TOEtPyP4 porphyrin on the stability and conductivity of bilayer lipid membranes

Cited by 4 publications

References 17 publications

Membrane-permeabilized sonodynamic therapy enhances drug delivery into macrophages

Membrane-permeabilized sonodynamic therapy enhances drug delivery into macrophages

Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

STABILITY AND CONDUCTIVITY OF BILAYER LIPID MEMBRANE IN PRESENCE $\mathrm{Al_2O_3}$ NANOPARTICLES

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