Influence of handling and/or anaesthesia on stress response in rainbow trout. Effects on liver primary metabolism

Morales, Amalia E.; Garcia-Rejon, L.; Higuera, Manuel de la

doi:10.1016/0300-9629(90)90014-j

Cited by 94 publications

(66 citation statements)

References 36 publications

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“…The return of plasma glucose levels to pre-stress baseline levels coincided with the 13 recovery of hepatic glycogen that could be explained by a stimulation of GSase activity linked to a decrease in GPase activity (Soengas et al, 1992a). These results agree with previous experiments that demonstrate the recovery of glycogen stores after a period of stress (Morales et al, 1990). In both confined HR and LR fish hepatic glycogen concentrations were higher than those in control fish at the end of the study.…”

Section: Discussionsupporting

confidence: 90%

“…al., 1984;Morales et al, 1990;Soengas et al, 1992bSoengas et al, , 1993Reubush and Heath, 1996). However, in this case the most pronounced elevation of plasma glucose was observed in the LR group and the hyperglycaemic response in the HR fish was muted, suggesting, on the basis of blood cortisol levels in the two lines, little direct involvement of cortisol in glucose mobilisation.…”

Section: Discussionmentioning

confidence: 69%

“…After 6 hours of confinement the decrease was followed by an elevation in plasma total amino acid concentration that in LR fish reached significantly higher values than HR, which only returned to pre-stress basal levels. A number of authors have reported an increase in plasma amino acids after exhaustive exercise, handling, or confinement (Morales et al, 1990;Braley and Anderson, 1992;Milligan, 1997;Vijayan et al, 1997). It has been shown that elevated cortisol levels can provoke a net catabolism of peripheral proteins (synthesis is also inhibited) and an elevation of plasma free amino acid levels would be followed by a gluconeogenic removal (van der Boon et al, 1991;Vijayan et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that elevated cortisol levels can provoke a net catabolism of peripheral proteins (synthesis is also inhibited) and an elevation of plasma free amino acid levels would be followed by a gluconeogenic removal (van der Boon et al, 1991;Vijayan et al, 1997). High plasma amino acid levels lead to induction of liver aminotransferases and consequently the participation of these metabolites in hepatic gluconeogenesis (Morales et al, 1990;van der Boon et al, 1991;Milligan, 1997). Several studies have provided evidence that alanine constitutes a good hepatic gluconeogenic precursor in fish (Medale et al, 1987;Braley and Anderson, 1992;Vijayan et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

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Divergence of endocrine and metabolic responses to stress in two rainbow trout lines selected for differing cortisol responsiveness to stress

Trenzado

Carrick

Pottinger

2003

General and Comparative Endocrinology

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Abstract. Rainbow trout (Oncorhynchus mykiss) of two lines selected for low (LR) and high (HR) cortisol stress-responsiveness were subjected to confinement for a period of 336 hours.Endocrine (plasma cortisol, hepatic cortisol binding) and metabolic (plasma glucose, lactate, amino acids; hepatic glycogen and alanine aminotransferase levels) indices of stress were measured at intervals in confined and unconfined fish of both lines. During confinement plasma cortisol concentration reached maximum values earlier in HR fish (2 hours) than in LR fish (6 hours) returning to control values within 336 hours in both lines. Paradoxically, although both HR and LR lines displayed a characteristic metabolic stress response, these changes were more pronounced in LR fish. Plasma glucose and lactate levels increased during confinement in both lines but to a significantly greater extent in LR fish. Confinement significantly elevated plasma amino acids to a greater extent in LR fish than in HR fish. Liver glycogen concentration was depleted most rapidly in LR fish but was significantly higher in confined fish of both lines than controls at the end of the experiment. No significant changes were observed in hepatic alanine aminotransferase activity during confinement. Confined fish of both lines displayed a decrease in hepatic cortisol receptor abundance within 24 h and this was more sustained in HR fish. The more pronounced disturbance of a broad range of indicators of stress in confined LR fish, compared to HR fish, throws doubt on the magnitude of the cortisol response being the primary driver of these differences.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Divergence of endocrine and metabolic responses to stress in two rainbow trout lines selected for differing cortisol responsiveness to stress

Trenzado

Carrick

Pottinger

2003

General and Comparative Endocrinology

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“…In both the control and perfused animals (once the perfusion was completed), the liver was dissected and weighed. Part of the liver (0.5 g) was homogenized in order to assess the lactate dehydrogenase (LDH) activity (Gollan et (Morales et al, 1990 (Frbhlich et al, 1973;Oshino et al, 1973;Oshino and Chance, 1975 (Keiding et al, 1980;Reed et al, 1982;Styne and Vierling, 1986). …”

Section: Sample Collectingmentioning

confidence: 99%

Perfusion of trout liver in situ. Description and validation of the technique

Cascales

Pérez‐Llamas²,

Marín³

et al. 1997

Reprod. Nutr. Dev.

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Summary ― The aim of this study was to develop a method for perfusing isolated trout livers that would make it possible to study hepatic metabolism in the whole organ. Rainbow trout (Oncorhynchus mykiss Walbaum) subjected to different fasting periods of 24, 48 or 96 h were used in all the experiments. A non-recirculating system was applied at a flow rate of 0.2 mL/min. The perfusion medium was a specific saline solution for salmonids oxygenated by a multibulb glass oxygenator. Viability assays included the measurement of oxygen consumption, lactate dehydrogenase activity and liver metabolic capacity. In addition, a histological study was carried out. Our results showed that the metabolic capacity of the liver survived throughout the perfusion process and that the functioning of this organ changed depending on the length of the fasting period to which the animal had been submitted. The method described here was shown to be suitable for studying the

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Time‐dependent effects of daily thermoperiods, feeding, and disturbances on scale allograft survival in the gulf killifish, Fundulus grandis

Nevid

Meier

1995

J. Exp. Zool.

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The length of time required to reject scale allografts was examined in groups of male gulf killifish (Fundulus grandis) treated daily with either 4-h thermoperiods (daily intervals of 30°C during a 20°C continuum), single meal feedings, or net-chasing disturbances at one of six different times after the onset of daily photoperiods (LD 12:12 or LD 14:lO). Scale allograft survival varied by 3040% in each timed stimulus experiment as a function of the time of day when the stimulus was provided. The phase relationship between two circadian neuroendocrine oscillations, previously proposed t o regulate physiologic and behavioral conditions in gulf killifish, may have important influences on the immune system as well. The phase of one oscillation is thought to be set by the daily photoperiod, and the other is preferentially set by the nonphotic daily Stimulus. 0 1995 Wiley-Liss, Inc.

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Influence of handling and/or anaesthesia on stress response in rainbow trout. Effects on liver primary metabolism

Cited by 94 publications

References 36 publications

Divergence of endocrine and metabolic responses to stress in two rainbow trout lines selected for differing cortisol responsiveness to stress

Divergence of endocrine and metabolic responses to stress in two rainbow trout lines selected for differing cortisol responsiveness to stress

Perfusion of trout liver in situ. Description and validation of the technique

Time‐dependent effects of daily thermoperiods, feeding, and disturbances on scale allograft survival in the gulf killifish, Fundulus grandis

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