Influence of Haptoglobin Polymorphism on Stroke in Sickle Cell Disease Patients

Edwards, Olivia; Burris, Alicia; Lua, Josh; Wilkie, Diana J.; Ezenwa, Miriam O.; Doré, Sylvain

doi:10.3390/genes13010144

Cited by 7 publications

(7 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Arboix et al [ 25 ] reported that patients who were young and had a prior history of venous thrombosis and recurrent stroke of undetermined cause were suspicious of hematological disease for stroke. Moreover, Hp 2 alleles are more disadvantageous than Hp1 alleles in sickle cell disease [ 26 ]. When considering that Hp is essential for reducing blood toxicity, oxidative stress, and inflammation in sickle cell disease, it is hypothesized that research on Hp is necessitated to find the clue and treatment for stroke, including ICH, in various hematologic diseases in the future.…”

Section: Discussionmentioning

confidence: 99%

Association of Haptoglobin Phenotypes with Outcomes in Patients with Spontaneous Intracerebral Hemorrhage

Jeon

Han

Kim

et al. 2022

Life

View full text Add to dashboard Cite

Object. We aimed to investigate the association of Haptoglobin (Hp) phenotypes with perihematomal edema (PHE) and neurological outcomes after intracerebral hemorrhage (ICH). Methods. This prospective multicenter study enrolled patients that suffered ICH from March 2017 to February 2020. Hp phenotypes were determined using Western blotting; relative α1 intensity was calculated in patients with Hp2-1. A multivariable logistic regression analysis was then conducted to identify risk factors for increased relative PHE at 96 h and 3-month poor outcomes. Results. In total, 120 patients were ultimately enrolled: Hp1-1 (n = 15, 12.5%); Hp2-1 (n = 51, 42.5%); and Hp2-2 (n = 54, 45.0%). Hp phenotype was significantly associated with PHE (p = 0.028). With Hp1-1 as a reference value, Hp2-2 significantly increased the likelihood of increased rPHE (OR = 6.294, 95% CI: 1.283–30.881), while Hp2-1 did not (OR = 2.843, 95% CI: 0.566–14.284). Poor outcomes were found to be closely associated with hematoma volume at admission (OR = 1.057, 95% CI: 1.015–1.101) and surgical treatment (OR = 5.340, 95% CI: 1.665–17.122) but not Hp phenotypes (p = 0.190). Further, a high level of relative α1 intensity was identified to be significantly associated with decreased rPHE (OR = 0.020, 95% CI: 0.001–0.358). However, the relative α1 intensity was not associated with poor outcomes (OR = 0.057, 95% CI: 0.001–11.790). Conclusions: ICH patients with Hp2-2 exhibited a higher likelihood of increased rPHE than those with Hp1-1. Higher relative α1 intensities were identified to be closely associated with rPHE in patients with Hp2-1.

show abstract

Section: Discussionmentioning

confidence: 99%

Association of Haptoglobin Phenotypes with Outcomes in Patients with Spontaneous Intracerebral Hemorrhage

Jeon

Han

Kim

et al. 2022

Life

View full text Add to dashboard Cite

show abstract

“…DNA Template and Polymerase Chain Reaction (PCR) : PCR technique was used to amplify the haptoglobine gene according to 11 and used the following Primers for the PCR technique. Primers (forward and reverse) as the table (1) the kit provide by Geneaid Genomic DNA extraction Kit company(Taiwan). All samples were submitted to three reactions (1, 2 and 3) and the genotypes were defined after electrophoresis on 1.5% agarose gel and identification of the amplified fragment for each allele.…”

Section: Blood Collectionmentioning

confidence: 99%

Effect of Haptoglobin Genotype on Essential Mineral Levels in Patients With Sickle Cell Anemia, Hepatitis C: A comparative Analysis

Salih Jumaa,

Abid Maktoof,

Salih Nuhia

2023

JEPS

View full text Add to dashboard Cite

AbstracteThe current study was conducted in the laboratories of the Marsh Research Center, the Center for Genetic Diseases, and Al-Hussein Teaching Hospital during the period from June 2022 to January 2023, with the aim of studying the genetic polymorphism of the haptoglobin (Hp) gene and its relationship to levels of essential minerals in patients with sickle cell anemia. Hepatitis C, and sickle cell anemia with hepatitis C. a total of 130 participants were classified, (40) control groups, (40) patients with sickle cell anemia, (40) patients with hepatitis C, and (10) sickle cell patients with hepatitis C .DNA was isolated and polymerase chain reaction (PCR) performed using genotype-specific primers for the three regions of the haptoglobin gene, and genotypes were determined after electrophoresis on agarose gels and determining the amplified fraction of each allele.Iron, copper, zinc, and chromium in blood serum were determined by flame atomic absorption spectrometry according to the manufacturer's procedures.The study found that the Hp2-2 genotype was more common in patients with sickle cell anemia, hepatitis, and both sickle cell anemia and hepatitis compared to the healthy control group.However, there were no significant differences in this genotype between the three diseases. In all patients, the level of iron and copper in the serum was significantly higher compared to the control group.and In all patients, the study showed a significant depletion of serum zinc compared to the control group,And no significant difference in serum chromium concentration between the patients and the control group.Regarding the polymorphisms of haptoglobin, the study did not find a statistically significant relationship between these genetic variations and the levels of iron, copper, and chromium in any of the participant groups. However, the concentrations of zinc differed between the three types of haptoglobin.

show abstract

“…Finally, the review entitled “Influence of Haptoglobin Polymorphism on Stroke in Sickle Cell Disease Patients” by Edwards et al is of substantial interest for our SI. This review outlines the current clinical research investigating how the haptoglobin (Hp) genetic polymorphism and stroke occurrence are implicated in sickle cell disease (SCD) pathophysiology [ 19 ]. Hp is an acute-phase protein capable of binding hemoglobin, thus preventing iron loss and renal damage [ 20 ].…”

mentioning

confidence: 99%

“…Hp is an acute-phase protein capable of binding hemoglobin, thus preventing iron loss and renal damage [ 20 ]. Edwards et al report the role of Hp in patients with SCD is critical in combating blood toxicity, inflammation, oxidative stress, and stroke [ 19 ]. IS occurs when a blocked vessel decreases blood-oxygen delivery to cerebral tissue and is commonly associated with SCD.…”

mentioning

confidence: 99%