Influence of heavy metals on glyceraldehyde‐3‐phosphate dehydrogenase interactions in <i>Chironomus riparius</i> larvae

Wai, Isaac; Chong, K C; Ho, Wingkin

doi:10.1002/etc.2265

Cited by 8 publications

(4 citation statements)

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“…Oxidation by H 2 O 2 inhibits the activity of several proteins including GAPDH and causes translocation of mammalian GAPDH to the nucleus, where it plays a role in DNA repair [ 23 ]. GAPDH is also reported to be inhibited by divalent metal ions [ 24 ], our current findings establish that M.tb GAPDH enzyme activity is inhibited by both H 2 O 2 and divalent metal ions. Inhibition by Cu 2+ and Cd 2+ was limited in the presence of 1 mM β-ME indicating the role of the active site cysteine residues.…”

Section: Discussionsupporting

confidence: 81%

Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

et al. 2016

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BackgroundObtaining sufficient quantities of recombinant M.tb proteins using traditional approaches is often unsuccessful. Several enzymes of the glycolytic cycle are known to be multifunctional, however relatively few enzymes from M.tb H37Rv have been characterized in the context of their enzymatic and pleiotropic roles. One of the primary reasons is the difficulty in obtaining sufficient amounts of functionally active protein.ResultsIn the current study, using M.tb glyceraldehyde-3-phosphate dehydrogenase (GAPDH) we demonstrate that expression in E. coli or M. smegmatis results in insolubility and improper subcellular localization. In addition, expression of such conserved multisubunit proteins poses the problem of heteromerization with host homologues. Importantly the expression host dramatically affected the yield and functionality of GAPDH in terms of both enzymatic activity and moonlighting function (transferrin binding). The applicability of this system was further confirmed using two additional enzymes i.e. M.tb Pyruvate kinase and Enolase.ConclusionsOur studies establish that the attenuated strain M.tb H37Ra is a suitable host for the expression of highly hydrophobic, conserved, multimeric proteins of M.tb H37Rv. Significantly, this expression host overcomes the limitations of E. coli and M. smegmatis expression and yields recombinant protein that is qualitatively superior to that obtained by traditional methods. The current study highlights the fact that protein functionality (which is an an essential requirement for all in vitro assays and drug development) may be altered by the choice of expression host.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-016-0537-0) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 81%

Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

et al. 2016

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“…C. riparius larvae inhabit small ditches and streams with increased carriage of organic matter, mostly in agricultural areas or streams receiving waste effluents from urban areas (Armitage et al., ; Calle‐Martínez & Casas, ). Consequently, C. riparius populations need to adapt to their local pollutants, that is, pesticides (Müller et al., ), metals (Pedrosa, Cocchiararo, Bordalo, et al., ; Wai, Chong, & Ho, ), organic pollution (Vogt et al., ) and its effects on physicochemical conditions of the water body as decreased oxygen content or hydrogen sulphide levels. Results of the functional enrichment analysis indicate that candidates of local adaptation are significantly enriched for genes that can be associated with detoxification processes, as, for example transport processes, phosphorylation, epigenetic response, immunity and larval development (Table , Figure ).…”

Section: Discussionmentioning

confidence: 99%

“…C. riparius larvae inhabit small ditches and streams with increased carriage of organic matter, mostly in agricultural areas or streams receiving waste effluents from urban areas (Armitage et al, 1997;Calle-Mart ınez & Casas, 2006). Consequently, C. riparius populations need to adapt to their local pollutants, that is, pesticides (M€ uller et al, 2012), metals (Pedrosa, Cocchiararo, Bordalo, et al, 2017;Wai, Chong, & Ho, 2013), organic pollution (Vogt et al, 2007) Figure 6). Some of these GO terms were also previously identified in studies with D. melanogaster (Table 1; Fabian et al, 2012;Kolaczkowski et al, 2011;.…”

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confidence: 99%

The genomic footprint of climate adaptation in Chironomus riparius

et al. 2018

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The gradual heterogeneity of climatic factors poses varying selection pressures across geographic distances that leave signatures of clinal variation in the genome. Separating signatures of clinal adaptation from signatures of other evolutionary forces, such as demographic processes, genetic drift and adaptation, to nonclinal conditions of the immediate local environment is a major challenge. Here, we examine climate adaptation in five natural populations of the harlequin fly Chironomus riparius sampled along a climatic gradient across Europe. Our study integrates experimental data, individual genome resequencing, Pool-Seq data and population genetic modelling. Common-garden experiments revealed significantly different population growth rates at test temperatures corresponding to the population origin along the climate gradient, suggesting thermal adaptation on the phenotypic level. Based on a population genomic analysis, we derived empirical estimates of historical demography and migration. We used an F outlier approach to infer positive selection across the climate gradient, in combination with an environmental association analysis. In total, we identified 162 candidate genes as genomic basis of climate adaptation. Enriched functions among these candidate genes involved the apoptotic process and molecular response to heat, as well as functions identified in studies of climate adaptation in other insects. Our results show that local climate conditions impose strong selection pressures and lead to genomic adaptation despite strong gene flow. Moreover, these results imply that selection to different climatic conditions seems to converge on a functional level, at least between different insect species.

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“…Consequently, C. riparius populations need to adapt to their local pollutants, i.e. pesticides (Müller et al 2012), metals (Pedrosa et al 2017a;Wai et al 2013), organic pollution (Vogt et al 2007), and its effects on physicochemical conditions of the water body as decreased oxygen content or hydrogen sulphide levels. Results of the functional enrichment analysis indicate that candidates of local adaptation are significantly enriched for genes that can be associated to detoxification processes, as for example transport processes, phosphorylation, epigenetic response, immunity, and larval development (Tab.…”

Section: Functional Basis Of Local and Clinal Adaptation In C Ripariusmentioning

confidence: 99%

The genomic footprint of climate adaptation inChironomus riparius

Oppold

Wieser

Schell

et al. 2017

Preprint

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The gradual heterogeneity of climatic factors pose varying selection pressures across geographic distances that leave signatures of clinal variation in the genome. Separating signatures of clinal adaptation from signatures of other evolutionary forces, such as demographic processes, genetic drift, and adaptation to non-clinal conditions of the immediate local environment is a major challenge. Here, we examine climate adaptation in five natural populations of the harlequin fly Chironomus riparius sampled along a climatic gradient across Europe. Our study integrates experimental data, individual genome resequencing, Pool-Seq data, and population genetic modelling. Common-garden experiments revealed a positive correlation of population growth rates corresponding to the population origin along the climate gradient, suggesting thermal adaptation on the phenotypic level. Based on a population genomic analysis, we derived empirical estimates of historical demography and migration. We used an F ST outlier approach to infer positive selection across the climate gradient, in combination with an environmental association analysis. In total we identified 162 candidate genes as genomic basis of climate adaptation. Enriched functions among these candidate genes involved the apoptotic process and molecular response to heat, as well as functions identified in other studies of climate adaptation in other insects. Our results show that local climate conditions impose strong selection pressures and lead to genomic adaptation despite strong gene flow. Moreover, these results imply that selection to different climatic conditions seems to converge on a functional level, at least between different insect species.

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Influence of heavy metals on glyceraldehyde‐3‐phosphate dehydrogenase interactions in Chironomus riparius larvae

Cited by 8 publications

References 23 publications

Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

The genomic footprint of climate adaptation in Chironomus riparius

The genomic footprint of climate adaptation inChironomus riparius

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