Influence of high-fat diet on host animal health via bile acid metabolism and benefits of oral-fed &lt;i&gt;Streptococcus thermophilus&lt;/i&gt; MN-ZLW-002

Luo, Yating; Cheng, Ruyue; Liang, Huijing; Miao, Zhonghua; Wang, Jiani; Zhou, Qingqing; Chen, Jianguo; He, Fang; Shi, Xi

doi:10.1538/expanim.21-0182

Cited by 5 publications

(3 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upstream of the eps gene cluster was deo D, encoding purine nucleotide phosphorylase, involved in the biosynthesis and catabolism of nucleotides, and similar to a purine nucleoside phosphatase in S. thermophilus LMD-9 (ID: Q03K54.1), with 100% sequence identity (Goh et al, 2011 ). As a transcriptional regulator of the LytR family, eps A had 97.81% homology with eps A (ID: ADQ63266.1) identified from S. thermophilus ND03 (Sun et al, 2011 ); eps B also showed a significant identity with eps B (97.62%) from S. thermophilus MN-ZLW-002 (ID: AFJ83638.1) (Luo et al, 2022 ). The gene eps C displayed a 99% identity with eps C in S. thermophilus M17PTZA496 (ID: ETW89010.1) and eps D showed a 99.86% identity with the eps D in S. thermophilus MTCC-5461 (ID: ELW74268.1) (Prajapati et al, 2013 ; Da Silva Duarte et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, three genes encoding glycosyltransferases were found, namely gene 0919, gene 0914, and gene 0911. Gene 0916 and gene 0918 are responsible for encoding capsular polysaccharide biosynthesis protein and respectively had a 98.83 and 99.74% sequence identity with the capsular synthesis protein gene in S. thermophilus ASCC 1275 (ID: AIC24645.1) and S. thermophilus MN-ZLW-002 (ID: AFJ83638.1) (Padmanabhan et al, 2020;Luo et al, 2022). Downstream of the eps gene cluster was orf 14.9 with a direction opposite to the eps gene cluster, which is associated with cell growth.…”

Section: Identification Of the Eps Biosynthetic Gene Clustermentioning

confidence: 99%

See 1 more Smart Citation

Genomic and transcriptomic analysis of genes involved in exopolysaccharide biosynthesis by Streptococcus thermophilus IMAU20561 grown on different sources of nitrogen

Wang,

Peng,

Liu

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Exopolysaccharides (EPSs), which are produced by lactic acid bacteria, have been found to improve the texture and functionality of fermented dairy products. In a previous study, four nitrogen sources were identified as affecting the yield, molecular weight and structure of EPSs produced by Streptococcus thermophilus IMAU20561 in M17 medium. In this genomic and transcriptomics study, a novel eps gene cluster responsible for assembly of repeating units of EPS is reported. This eps cluster (22.3 kb), consisting of 24 open reading frames, is located in the chromosomal DNA. To explore the biosynthetic mechanisms in EPS, we completed RNA-seq analysis of S. thermophilus IMAU20561 grown in four different nitrogen sources for 5 h (log phase) or 10 h (stationary phase). GO functional annotation showed that there was a significant enrichment of differentially expressed genes (DEGs) involved in: amino acid biosynthesis and metabolism; ribonucleotide biosynthesis and metabolism; IMP biosynthesis and metabolism; and phosphorus metabolism. KEGG functional annotation also indicated enrichment of DEGs involved in amino acid biosynthesis, glycolysis, phosphotransferase system, fructose, and mannose metabolism. Our findings provide a better understanding the genetic traits of S. thermophilus, the biosynthetic pathways needed for the production of EPS, and a theoretical basis for screening dairy starter cultures.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Identification Of the Eps Biosynthetic Gene Clustermentioning

confidence: 99%

Genomic and transcriptomic analysis of genes involved in exopolysaccharide biosynthesis by Streptococcus thermophilus IMAU20561 grown on different sources of nitrogen

Wang,

Peng,

Liu

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, Streptococcus thermophilus MN-ZLW-002 regulates gut microbiota by adjusting the composition of BAs and reduces the levels of high lipid and glucose. ( Luo et al, 2022b ).…”

Section: Discussionmentioning

confidence: 99%

Ji-Ni-De-Xie ameliorates type 2 diabetes mellitus by modulating the bile acids metabolism and FXR/FGF15 signaling pathway

Tao,

Peng,

Wang

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

Introduction: Ji-Ni-De-Xie (JNDX) is a traditional herbal preparation in China. It is widely used to treat type 2 diabetes mellitus (T2DM) in traditional Tibetan medicine system. However, its antidiabetic mechanisms have not been elucidated. The aim of this study is to elucidate the underlying mechanism of JNDX on bile acids (BAs) metabolism and FXR/FGF15 signaling pathway in T2DM rats.Methods: High-performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QQQ-MS) and UPLC-Q-Exactive Orbitrap MS technology were used to identify the constituents in JNDX. High-fat diet (HFD) combined with streptozotocin (45 mg∙kg−1) (STZ) was used to establish a T2DM rat model, and the levels of fasting blood-glucose (FBG), glycosylated serum protein (GSP), homeostasis model assessment of insulin resistance (HOMA-IR), LPS, TNF-α, IL-1β, IL-6, TG, TC, LDL-C, HDL-C, and insulin sensitivity index (ISI) were measured to evaluate the anti-diabetic activity of JNDX. In addition, metagenomic analysis was performed to detect changes in gut microbiota. The metabolic profile of BAs was analyzed by HPLC-QQQ-MS. Moreover, the protein and mRNA expressions of FXR and FGF15 in the colon and the protein expressions of FGF15 and CYP7A1 in the liver of T2DM rats were measured by western blot and RT-qPCR.Results: A total of 12 constituents were identified by HPLC-QQQ-MS in JNDX. Furthermore, 45 chemical components in serum were identified from JNDX via UPLC-Q-Exactive Orbitrap MS technology, including 22 prototype components and 23 metabolites. Using a T2DM rat model, we found that JNDX (0.083, 0.165 and 0.33 g/kg) reduced the levels of FBG, GSP, HOMA-IR, LPS, TNF-α, IL-1β, IL-6, TG, TC, and LDL-C, and increased ISI and HDL-C levels in T2DM rats. Metagenomic results demonstrated that JNDX treatment effectively improved gut microbiota dysbiosis, including altering some bacteria (e.g., Streptococcus and Bacteroides) associated with BAs metabolism. Additionally, JNDX improved BAs disorder in T2DM rats, especially significantly increasing cholic acid (CA) levels and decreasing ursodeoxycholic acid (UDCA) levels. Moreover, the protein and mRNA expressions of FXR and FGF15 of T2DM rats were significantly increased, while the expression of CYP7A1 protein in the liver was markedly inhibited by JNDX.Discussion: JNDX can effectively improve insulin resistance, hyperglycemia, hyperlipidemia, and inflammation in T2DM rats. The mechanism is related to its regulation of BAs metabolism and activation of FXR/FGF15 signaling pathway.

show abstract

Protective Effect of <i>Bifidobacterium longum</i> and <i>Streptococcus thermophilus</i> against Simvastatin-Induced Rhabdomyolysis in Hypercholesteraemic Rats

Raja,

Kumari,

Rani

2024

View full text Add to dashboard Cite

Simvastatin (SMV), a commonly prescribed drug for lowering lipid levels, is linked to the serious side effect of rhabdomyolysis. This study explores the potential of probiotics, specifically Bifidobacterium longum (BL) and Streptococcus thermophilus (ST), as supplementary treatments to alleviate simvastatin-induced rhabdomyolysis in rats with high cholesterol levels. This study assesses the effects of combining simvastatin with probiotics on parameters such as lipid profiles, renal function, skeletal muscle markers, inflammatory cytokines, and histological characteristics. Rats with elevated cholesterol levels were exposed to SMV treatment alone and in conjunction with probiotics. This study compared the effects of combining simvastatin with BL and ST, focusing on their potential to ameliorate SMV-induced rhabdomyolysis. Combining simvastatin with BL and ST yielded notable outcomes. The supplementation significantly improved lipid profiles by reducing atherogenic lipids and increasing cardioprotective HDL-C levels. Additionally, the probiotics, particularly ST and BL, showed indications of preserving renal function and mitigating the adverse effects of simvastatin on muscle health. Analysis of inflammatory cytokines suggested that probiotics may modulate inflammation. Histological assessments confirmed the protective effects of probiotics by maintaining tissue integrity and normal cell appearance. While BL exhibited a slight advantage over ST, both probiotics demonstrated similar potential as adjunction therapies. This study’s findings highlight the promising role of probiotics, specifically BL and ST, in ameliorating simvastatin-induced rhabdomyolysis. These probiotics show the potential to improve lipid profiles, safeguard renal function, preserve muscle health, modulate inflammation, and maintain tissue integrity. These results provide a hopeful basis for potential therapeutic interventions in individuals experiencing adverse effects associated with SMV treatment.

show abstract

Influence of high-fat diet on host animal health via bile acid metabolism and benefits of oral-fed <i>Streptococcus thermophilus</i> MN-ZLW-002

Cited by 5 publications

References 72 publications

Genomic and transcriptomic analysis of genes involved in exopolysaccharide biosynthesis by Streptococcus thermophilus IMAU20561 grown on different sources of nitrogen

Genomic and transcriptomic analysis of genes involved in exopolysaccharide biosynthesis by Streptococcus thermophilus IMAU20561 grown on different sources of nitrogen

Ji-Ni-De-Xie ameliorates type 2 diabetes mellitus by modulating the bile acids metabolism and FXR/FGF15 signaling pathway

Protective Effect of <i>Bifidobacterium longum</i> and <i>Streptococcus thermophilus</i> against Simvastatin-Induced Rhabdomyolysis in Hypercholesteraemic Rats

Contact Info

Product

Resources

About