Abstract-Micrometer and sub-micrometer sized non-magnetic particles were manipulated by an external strong magnetic field (e.g., 10 Tesla) with a high gradient. During the strong magnetic field effects, segregation of the non-magnetic particles was observed which could not be realised only with gravitational field. Numerical calculations were subsequently carried out to understand the effects on the insulating particles in a conductive liquid matrix. The migration of micrometer sized particles is obviously enhanced by the magnetic field gradient. Combining the experimental results and theoretical analysis, particle-particle magnetic interaction was found to influence the overall segregation of the particles as well. Magnetised by the strong magnetic field, magnetic interaction between non-magnetic particles becomes dominant and a self-assembly behavior can be demonstrated. Various factors such as the magnetic dipole-dipole interaction and chainchain interaction, are governing the particles assembly. According to calculations, magnetic field should be strong enough, at least 7 T in order to obtain the assembly morphologies in the present case.