Influence of Histamine H1 Receptor Antagonists on Thioredoxin Production In vitro and In vivo

Mizuyoshi, Tomomi; Asano, Masayo; Furuta, Atsuko; Asano, Koichiro; Kobayashi, Hitome

doi:10.4172/2155-6121.1000257

Cited by 1 publication

(1 citation statement)

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“…Moreover, it may be possible that levocetirizine inactivates ROS through direct scavenging activity or via activation of SOD, the only antioxidant enzyme that can scavenge superoxide [ 30 ]. Interestingly, it has also been shown that levocetirizine enhanced production of thioredoxin, a natural ROS scavenger, in hydrogen peroxide-stimulated macrophages [ 31 ]. Furthermore, levocetirizine may attenuate oxidant generation by reducing alveolar infiltration of inflammatory cells.…”

Section: Discussionmentioning

confidence: 99%

Levocetirizine Pretreatment Mitigates Lipopolysaccharide-Induced Lung Inflammation in Rats

Fahmi

Shehatou

Salem

2018

BioMed Research International

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This research was conducted to investigate possible protective influences of levocetirizine, a nonsedating H1 antihistamine, against lipopolysaccharide (LPS)-induced lung injury in rats. Male Sprague Dawley rats received either levocetirizine (1 mg/kg/day, orally) or the vehicle of the drug (2 ml/kg/day, orally) for 1 week before a single IP injection of LPS (7.5 mg/kg). A group of normal rats served as control. The experiments were terminated 18 h after the LPS challenge. Serum C-reactive protein levels were determined. Moreover, total cell count, lactate dehydrogenase (LDH) activity, protein levels, and total NOx were evaluated in bronchoalveolar lavage fluid (BALF). Pulmonary edema was evaluated as the wet/dry lung weight ratio. Lung tissue homogenate was assessed for antioxidant/pro-oxidant status. BALF and lung tissue levels of tumor necrosis factor-α (TNF-α) were assessed. Lungs were examined for histological alterations. LPS-mediated lung injury was manifested by pulmonary edema, leukocyte infiltration, oxidative stress, and inflammation. Levocetirizine attenuated lung edema and mitigated the increases in BALF protein levels, LDH activity, and lung leukocyte recruitment in LPS-challenged rats. Additionally, TNF-α protein levels in BALF and lung tissue were diminished by levocetirizine administration. Levocetirizine also exhibited a potent antioxidant activity as indicated by a decrease in lung tissue levels of malondialdehyde and an enhancement of superoxide dismutase activity. Histological examination of lung tissues confirmed the beneficial effect of levocetirizine against LPS-induced histopathological alterations. In conclusion, levocetirizine may offer protection against lung tissue damage and inflammation in LPS-challenged rats.

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Section: Discussionmentioning

confidence: 99%