Influence of Host and Geographical Regions on the Rumen Methanogens Diversity in Indian Cattle and Buffaloes

Trivedi, Shraddha; Malik, P. K.; Kolte, Atul P.; Thirumalaisamy, G; Kumar, Vanitha Shyamili; Sejian, Veerasamy; Dhali, A.; Bhatta, Raghavendra

doi:10.21203/rs.3.rs-53542/v1

Cited by 2 publications

(4 citation statements)

References 52 publications

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“…A meta-analysis [ 65 ], concluded that the methanogens associated with the genus Methanobrevibacter are similarly distributed in cattle and buffaloes. Further, similar distributions of Methanobrevibacter in Indian cattle and buffaloes belonging to two distinct geographical regions were reported in a recent study by Trivedi et al [ 55 ]. The above studies reporting the similar proportion of Methanobrevibacter were either conducted separately in cattle and buffaloes or both the species were fed on different diets or having uncontrolled environmental conditions prevailing in different geographical regions; hence, these previous studies did not reveal the actual impact of the hosts on the proportion of ruminal methanogens.…”

Section: Discussionsupporting

confidence: 85%

“…In our study, Group 8 methylotrophs were exclusively identified in the cattle. The greater proportion of Group 8 methanogens in cattle compared to their distribution in buffaloes has recently been confirmed [ 55 ]. This difference should be taken into account while developing strategies for methane mitigation from cattle and buffaloes.…”

Section: Discussionmentioning

confidence: 98%

“…Their findings confirmed that the ruminal archaea community are less diversified than the bacteria and a major fraction of the archaea remain invariable across geographical locations [ 54 ]. More recently, Trivedi et al [ 55 ] established that the geographical region and host influence the archaeal community composition. The multiple factors (diet, host, geographical locations) were confounded in most of these studies and due to the variability of more than one factor, it is very difficult to clearly separate the impact of host or diet or geographical locations on the ruminal methanogens community structure.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet

et al. 2021

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An in vivo study was conducted to compare the enteric methane emissions and diversity of ruminal methanogens in cattle and buffaloes kept in the same environment and fed on the same diet. Six cattle and six buffaloes were fed on a similar diet comprising Napier (Pennisetum purpureum) green grass and concentrate in 70:30. After 90 days of feeding, the daily enteric methane emissions were quantified by using the SF6 technique and ruminal fluid samples from animals were collected for the diversity analysis. The daily enteric methane emissions were significantly greater in cattle as compared to buffaloes; however, methane yields were not different between the two species. Methanogens were ranked at different taxonomic levels against the Rumen and Intestinal Methanogen-Database. The archaeal communities in both host species were dominated by the phylum Euryarchaeota; however, Crenarchaeota represented <1% of the total archaea. Methanogens affiliated with Methanobacteriales were most prominent and their proportion did not differ between the two hosts. Methanomicrobiales and Methanomassillicoccales constituted the second largest group of methanogens in cattle and buffaloes, respectively. Methanocellales (Methanocella arvoryza) were exclusively detected in the buffaloes. At the species level, Methanobrevibacter gottschalkii had the highest abundance (55–57%) in both the host species. The relative abundance of Methanobrevibacter wolinii between the two hosts differed significantly. Methanosarcinales, the acetoclastic methanogens were significantly greater in cattle than the buffaloes. It is concluded that the ruminal methane yield in cattle and buffaloes fed on the same diet did not differ. With the diet used in this study, there was a limited influence (<3.5%) of the host on the structure of the ruminal archaea community at the species level. Therefore, the methane mitigation strategies developed in either of the hosts should be effective in the other. Further studies are warranted to reveal the conjunctive effect of diet and geographical locations with the host on ruminal archaea community composition.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet

et al. 2021

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“…Another recent report from Seshadri et al (2018) showed that hydrogenotrophic methanogens accounted for 78% of ruminal methanogens, whereas methylotrophic methanogens accounted for the remaining 22%. A comparison of methanogens inhabiting the rumen of water buffaloes and cattle (Trivedi et al, 2020) showed differences in the relative abundance of individual methanogens, although similar methanogens (diversity) were observed as described in previous reports. Methanobrevibacter gottschalkii was predominant in the rumen of water buffaloes (30-55%) followed by Methanobrevibacter ruminantium (5-10%) and Methanosphaera ISO3-F5 (2-5%), although regional differences were noted in the relative abundance of individual populations among water buffaloes.…”

Section: Global Efforts Needed To Understand Methanogen Diversitysupporting

confidence: 84%

Symposium review: Understanding the role of the rumen microbiome in enteric methane mitigation and productivity in dairy cows

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Indugu

Narayan

et al. 2022

Journal of Dairy Science

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Ruminants are one of the largest sources of global CH 4 emissions. This enteric CH 4 is exclusively produced by methanogenic archaea as a natural product during microbial fermentation in the reticulorumen. As CH 4 formation leads to a gross energy loss for the ruminant host and is also an environmental issue, several CH 4 mitigation approaches have been investigated, but results have been inconsistent, which may be partially attributed to a lack of understanding of the mechanistic basis of methanogenesis and the effect of inhibitors on individual methanogenic lineages and other fermenting microbes in the rumen. Methanogenic archaea are obligatory anaerobes that can reduce CO 2 , methanol, or methylamines or cleave acetate to form CH 4 . Although methanogens work toward a common goal of generating energy through the formation of CH 4 , individual methanogenic lineages differ in their physiological and metabolic capabilities, which can differentially affect H 2 transactions and CH 4 formation. Using advanced omic approaches, recent research has revealed that less abundant methanol-utilizing Methanosphaera and methylamine-and methanol-utilizing Methanomassiliicoccales lineages are positively correlated with CH 4 emissions and may have a greater share in overall CH 4 production compared with more abundant CO 2 -reducing methanogens than previously thought. These data imply that the diversity as well as the abundance of methanogens is important in CH 4 formation, and that this diversity is influenced by H 2 availability and interactions within and between H 2 -producing microbes in the rumen. These complex interactions between microbes and H 2 are further influenced by variations in dietary, host, and environmental conditions. This review discusses critical knowledge gaps underlying methanogen diver-sity and its link to CH 4 formation, formation of specific bacteria-archaeal cohorts, and how H 2 production and utilization are regulated between these cohorts during normal and inhibited methanogenesis. Addressing these knowledge gaps has the potential to lead to the development of novel strategies or to complement existing strategies to effectively reduce CH 4 formation while also improving productivity in dairy cows.

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Influence of Host and Geographical Regions on the Rumen Methanogens Diversity in Indian Cattle and Buffaloes

Cited by 2 publications

References 52 publications

Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet

Comparison of enteric methane yield and diversity of ruminal methanogens in cattle and buffaloes fed on the same diet

Symposium review: Understanding the role of the rumen microbiome in enteric methane mitigation and productivity in dairy cows

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