Influence of HSA on micellization of NLSS and BC: An experimental-theoretical approach of its binding characteristics

Fatma, Ishrat; Sharma, Vivek; Malik, Nisar Ahmad; Assad, Humira; Cantero-López, Plinio; Sánchez, Julio; López-Rendón, Roberto; Yáñez, Osvaldo; Thakur, Ramesh; Kumar, Ashish

doi:10.1016/j.molliq.2022.120532

Cited by 5 publications

(1 citation statement)

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“…As shown in [ 43 , 44 ], the use of mixed surfactant–protein systems leads to a synergistic effect in self-assembly behavior and solubility of lipophilic compounds and, consequently, improves their biocompatibility. Meanwhile, few publications are available on the self-assembly and morphological behavior of the HSA–surfactant systems [ 45 , 46 , 47 ], which provide evidence of the deficiency of fundamental information in this field and highlight the novelty of the data expected.…”

Section: Introductionmentioning

confidence: 99%

Role of Polyanions and Surfactant Head Group in the Formation of Polymer–Colloid Nanocontainers

et al. 2023

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Objectives. This study was aimed at the investigation of the supramolecular systems based on cationic surfactants bearing cyclic head groups (imidazolium and pyrrolidinium) and polyanions (polyacrylic acid (PAA) and human serum albumin (HSA)), and factors governing their structural behavior to create functional nanosystems with controlled properties. Research hypothesis. Mixed PE–surfactant complexes based on oppositely charged species are characterized by multifactor behavior strongly affected by the nature of both components. It was expected that the transition from a single surfactant solution to an admixture with PE might provide synergetic effects on structural characteristics and functional activity. To test this assumption, the concentration thresholds of aggregation, dimensional and charge characteristics, and solubilization capacity of amphiphiles in the presence of PEs have been determined by tensiometry, fluorescence and UV-visible spectroscopy, and dynamic and electrophoretic light scattering. Results. The formation of mixed surfactant–PAA aggregates with a hydrodynamic diameter of 100–180 nm has been shown. Polyanion additives led to a decrease in the critical micelle concentration of surfactants by two orders of magnitude (from 1 mM to 0.01 mM). A gradual increase in the zeta potential of HAS–surfactant systems from negative to positive value indicates that the electrostatic mechanism contributes to the binding of components. Additionally, 3D and conventional fluorescence spectroscopy showed that imidazolium surfactant had little effect on HSA conformation, and component binding occurs due to hydrogen bonding and Van der Waals interactions through the tryptophan amino acid residue of the protein. Surfactant–polyanion nanostructures improve the solubility of lipophilic medicines such as Warfarin, Amphotericin B, and Meloxicam. Perspectives. Surfactant–PE composition demonstrated beneficial solubilization activity and can be recommended for the construction of nanocontainers for hydrophobic drugs, with their efficacy tuned by the variation in surfactant head group and the nature of polyanions.

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