Influence of humectants on the thermotropic behaviour and nanostructure of fully hydrated lecithin bilayers

Li, Ngai Ying Denise; Moore, David J.; Thompson, Michael A.; Welfare, Eloise H.; Rappolt, Michael

doi:10.1016/j.chemphyslip.2021.105165

Cited by 4 publications

(5 citation statements)

References 49 publications

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“…Using eqs and , we calculated the area per chain, A C , in the pure α phase and the pure β′ phase. Clearly, A C decays from 19.4 to 18.8 Å 2 , which is in good agreement with other reported hydrocarbon chain packings. , …”

Section: Resultssupporting

confidence: 92%

“…Clearly, A C decays from 19.4 to 18.8 Å 2 , which is in good agreement with other reported hydrocarbon chain packings. 45,46 We note that our BuSP powder sample was received in a temperature-controlled package, and it was then kept in the freezer for more than one month before measurement. Therefore, we argue that the sample should be the most stable crystal form of this asymmetrical TAG.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Polymorphism of a Highly Asymmetrical Triacylglycerol in Milk Fat: 1-Butyryl 2-Stearoyl 3-Palmitoyl-glycerol

Pratama

Burholt

Baker

et al. 2022

Crystal Growth & Design

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Milk fat has more than 200 triacylglycerols (TAGs), which play a pivotal role in its crystallization behavior. Asymmetrical TAGs containing short butyryl chains contribute to a significant portion of milk fat TAGs. This work aims to elucidate the crystallization behavior of asymmetrical milk fat TAGs by employing the pure compound of 1-butyryl 2-stearoyl 3-palmitoylglycerol (BuSP). The structural evolution of BuSP after being cooled down to 20 °C from the melt is evaluated by small-and wide-angle X-ray scattering (SAXS and WAXS) and differential scanning calorimetry (DSC). The temporal structural observation shows that BuSP crystallizes into the α-form with short and long spacings of 4.10 and 56.9 Å, respectively, during the first hour of isothermal hold at 20 °C. The polymorphic transformation of the α to β′ phase occurred after 4 h of isothermal hold, and the β′to α-form fraction ratio was about 70:30 at the end of the isothermal experiment (18 h). Pure β′-form X-ray patterns are obtained from the BuSP powder with short spacings of 4.33, 4.14, and 3.80 Å, while the long spacing of 51.2 Å depicts a three-chain-length lamellar structure with a tilt angle of 32°. Corresponding DSC measurements display that BuSP crystallizes from the melt at 29.1 °C, whereas the melting of αand β′-forms was recorded at 30.3 and 47.8 °C, respectively. In the absence of the β-form, the β′-polymorph is the most stable observed form in BuSP. This work exemplarily explains the crystallization behavior of asymmetrical milk fat TAGs and thus provides new insights into their role in overall milk fat crystallization.

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Section: Resultssupporting

confidence: 92%

Section: ■ Results and Discussionmentioning

confidence: 99%

Polymorphism of a Highly Asymmetrical Triacylglycerol in Milk Fat: 1-Butyryl 2-Stearoyl 3-Palmitoyl-glycerol

Pratama

Burholt

Baker

et al. 2022

Crystal Growth & Design

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“…For instance, an increase in the area per lipid decreases the effective surface charge density in the headgroup region, and consequently increases the dielectric permittivity in the interstitial water region. [92][93][94] This increase in permittivity will produce a reduction in the strength of the VdW interaction and allow the water spacing to increase, which is observed in the swelling of PC MLVs.…”

Section: Headgroup Influence On the Interlamellar Water Regionsmentioning

confidence: 99%

Planar confined water organisation in lipid bilayer stacks of phosphatidylcholine and phosphatidylethanolamine

et al. 2023

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“…The area per lipid also needs to be considered in the strength of the Hamaker constant. For instance, an increase in the area per lipid decreases the effective surface charge density in the headgroup region, and consequently increases the dielectric permittivity in the interstitial water region [92][93][94] . This increase in permittivity will produce a reduction in the strength of the VdW interaction and allow the water spacing to which is observed in the swelling of PC MLVs.…”

Section: Headgroup Influence On the Interlamellar Water Regionsmentioning

confidence: 99%

Planar Confined Water Organisation in Lipid Bilayer Stacks of Phosphatidylcholine and Phosphatidylethanolamine

Vancuylenberg

Sadeghpour

Tyler

et al. 2023

Preprint

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Phospholipid-based liposomes are abundantly studied in biomembrane research and used in numerous medical and biotechnological applications. Despite current extensive knowledge on membrane nanostructure and its mechanical properties under various environmental conditions, there is still a lack of understanding on interfacial lipid-water interactions. In this work, the nature of the confined water layer for L-α-phosphatidylcholine (egg-PC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dimyristoyl-sn-glycerol-3-phosphatidyl-choline (DMPC) and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine (DMPE) in the fluid lamellar phase of multilamellar vesicles was investigated. A new model for describing three different water regions is proposed, which have been characterised using a combination of small angle X-ray scattering (SAXS) and densitometry. The three regions concern (i) ‘the headgroup water’, (ii) ‘perturbed water’ near the membrane interface and (iii) a core layer of ‘free water’ (unperturbed water). The behaviour of all three layers is discussed as a function of temperature, concerning influences of chain saturation and headgroup type. While the overall water layer and perturbed water layer thickness increase with temperature, the free water layer displays the opposite trend for PCs, and in PEs is completely absent. Furthermore, an estimate of the temperature dependent headgroup orientation is given for both, PCs and PEs. The newly presented structural data deduced from the three-water region model will be useful for future refined molecular dynamics simulations and allow a better theoretical understanding of the attractive van der Waals force between adjacent membranes.

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Influence of humectants on the thermotropic behaviour and nanostructure of fully hydrated lecithin bilayers

Cited by 4 publications

References 49 publications

Polymorphism of a Highly Asymmetrical Triacylglycerol in Milk Fat: 1-Butyryl 2-Stearoyl 3-Palmitoyl-glycerol

Polymorphism of a Highly Asymmetrical Triacylglycerol in Milk Fat: 1-Butyryl 2-Stearoyl 3-Palmitoyl-glycerol

Planar confined water organisation in lipid bilayer stacks of phosphatidylcholine and phosphatidylethanolamine

Planar Confined Water Organisation in Lipid Bilayer Stacks of Phosphatidylcholine and Phosphatidylethanolamine

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