Influence of<i>Emblica officinalis</i>aqueous extract on growth and antioxidant defense system of human hepatoma cell line (HepG2)

Shivananjappa, Mahesh; Joshi, Manoj Kumar

doi:10.3109/13880209.2011.618501

Cited by 47 publications

(35 citation statements)

References 27 publications

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“…The sieved powder was extracted in water in a ratio of 1:8 by boiling for 4 h. The extracts were subsequently filtered through four layers of muslin cloth, concentrated and spray dried. The fingerprint analysis of four independent batches of extracts using HPLC and NMR to demonstrate the consistency across preparations was performed and has been reported previously …”

Section: Methodsmentioning

confidence: 99%

“…Supernatants (cytosol) were stored in −80 °C for further analysis. Separate aliquots were used for each parameter to avoid repeated freeze–thaw cycle . Protein concentration in cytosol was determined using a kit from the Cayman Chemical Company (CCC), USA, as per manufacturer's specification.…”

Section: Methodsmentioning

confidence: 99%

“…The study of the molecular basis of the antioxidant response may benefit from the use of established cell culture such as HepG2 . Earlier, we demonstrated that the aqueous extract of Terminalia arjuna (AETA) has the propensity to increase endogenous antioxidant enzymes activity and to reduce levels of lipid hydroperoxides (LHPs) in HepG2 cells . To unravel the intimate mechanisms by which Ayurvedic herbs regulate antioxidant defenses of cells, it is necessary to study the response of the cell defense system to pro‐oxidants that modify the intracellular pro‐oxidant–antioxidant balance in the presence and absence of such herbs.…”

Section: Introductionmentioning

confidence: 99%

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Aqueous extract of Terminalia arjuna attenuates tert‐butyl hydroperoxide–induced oxidative stress in HepG2 cell model

Shivananjappa¹,

Mhasavade²,

Joshi

2012

Cell Biochemistry & Function

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Arjuna (Terminalia arjuna) is a medicinal plant used in many polyherbal hepatoprotective formulations. Although widely claimed to be antioxidant, data supporting such actions of Arjuna are limited. In the present study, we have investigated the efficacy of the aqueous extract of T. arjuna (AETA) using a standard pro-oxidant [tertiary butyl hydroperoxide (TBHP)] in HepG2 cells. Cells were incubated with AETA (5-100 µg/ml) for a range of time points (4-24 h) with or without TBHP (500 μM), and biochemical markers of oxidative stress (OS) were determined. Cells incubated with TBHP showed the significant induction of OS response in cytosol manifested as lipid hydroperoxide (76%-198%) and the generation of reactive oxygen species (60%-127%). Diminished levels of reduced glutathione (35%-60%) and total antioxidant capacity (20%-61%) suggested an altered redox state. Significant perturbations in the activities of antioxidant enzymes such as catalase (30%-56%), superoxide dismutase (25%-68%), glutathione S-transferase (29%-67%), glutathione peroxidase (24%-68%) and glutathione reductase (38%-49%) were discernible suggesting the ongoing OS in the cells. However, cells treated with AETA (100 µg/ml) along with TBHP offered significant protection by reducing levels of lipid hydroperoxide (33%-62%) and ROS (69%) and by increasing antioxidant capacity (54%-81%) and levels of reduced glutathione (49%-82%). Further, it also enhanced the activities of endogenous antioxidant enzymes (superoxide dismutase, 60%; catalase, 35%-82%; glutathione peroxidase, 42-65 %; glutathione reductase, 48%-62%; and glutathione S-transferase, 22%-100%). Taken together, these data suggest that Arjuna can protect against the oxidative damage induced by TBHP and may be effectively used as a hepatoprotective adjuvant to abrogate OS in vivo.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aqueous extract of Terminalia arjuna attenuates tert‐butyl hydroperoxide–induced oxidative stress in HepG2 cell model

Shivananjappa¹,

Mhasavade²,

Joshi

2012

Cell Biochemistry & Function

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“…Substantial reduction in the levels of lipid hydroperoxide and reactive oxygen species (ROS) was observed in the study that incubated E. officinalis for 24 h. Moreover, E. officinalis increased the levels of GSH, antioxidant capacity and activities of antioxidant enzymes (SOD; CAT; GSH peroxidase; GSH reductase; and GSH S-transferase). [7] Additionally, when administered once daily for 7 days the active tannoids of E. officinalis induced a rise in both frontal cortical as well as striatal SOD, CAT and GSH peroxidase (GPX) activity, with associated reduction in lipid peroxidation in these brain areas. The results also specify that the antioxidant activity of E. officinalis may reside in the tannoids of the fruits of the plant, which have vitamin C-like properties, rather than vitamin C itself.…”

Section: Antioxidantmentioning

confidence: 99%

Emblica officinalis(Amla): A review of potential therapeutic applications

Bhandari¹,

Kamdod²

2012

Int J Green Pharm

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PHYTOCHEMISTRYE. officinalis is one of the most extensively studied plants and reports suggest that it contains tannins, alkaloids, and phenolic compounds. It has been reported that fruits of E. officinalis contains higher amount of vitamin C and considerably higher concentrations of most minerals, protein and amino acids like glutamic acid, proline, aspartic acid, alanine, cystine and lysine. vitamin C levels are more than those in oranges, tangerines, or lemons. Fresh pericarp of E. officinalis contains higher amount of hydrolysable tannins like emblicanin A and B, punigluconin, pedunculagin. Activity-directed fractionation and purification process identified phytochemicals present in E. officinalis. They have identified gallic acid, methyl gallate, corilagin, furosin and geraniin in E. officinalis by a chromatographic and spectroscopic method. Phytochemical investigations revealed that E. officinalis contains higher amount of flavonoid like quercetin. Fruits were also analyzed for their alkaloidal Emblica officinalis Gaertn. or Phyllanthus emblica Linn, commonly known as Indian gooseberry or Amla, is perhaps the most important medicinal plant in the Indian traditional system of medicine, the Ayurveda. Several parts of the plant are used to treat a variety of diseases, but the most important is the fruit. Many ailments are treated by the fruit which is used either alone or in combination with other plants. These include common cold and fever; as a diuretic, laxative, liver tonic, refrigerant, stomachic, restorative, alterative, antipyretic, anti-inflammatory, hair tonic; to prevent peptic ulcer and dyspepsia, and as a digestive. E. officinalis possesses antipyretic, analgesic, antitussive, antiatherogenic, adaptogenic, cardioprotective, gastroprotective, antianemic, antihypercholesterolemic, wound healing, antidiarrheal, antiatherosclerotic, hepatoprotective, nephroprotective, and neuroprotective properties as demonstrated in numerous preclinical studies. Furthermore, experimental studies have reported that E. officinalis and some of its phytochemicals also exhibit anticarcinogenic properties. E. officinalis is also reported to possess radiomodulatory, chemomodulatory, chemopreventive, free radical scavenging, antioxidant, anti-inflammatory, antimutagenic and immunomodulatory activities. These properties are efficacious in the treatment and prevention of cancer. This review summarizes the results related to these properties and also emphasizes the aspects that warrant future research establishing its activity and utility as a cancer preventive and therapeutic drug in humans.

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“…The P. emblica fruits are the rich source of ascorbic acid (vitamin C), which itself has well-documented nutritional and medicinal properties (1). ANTICANCER Recent investigations show that P. emblica possesses several biological effects of pharmacologic importance, like antiinflammatory (2,(5)(6)(7), antimutagenic (2), antitumor (8)(9)(10), analgesic and antipyretic (11,12), chemopreventive and hepatoprotective (13), antifungal and antimicrobial (4,(14)(15)(16)(17), antioxidant activities (18)(19)(20). P. emblica contains as many as 18 main compounds that showed significant antiproliferative activity against various tumor cells (10,21).…”

Section: Introductionmentioning

confidence: 99%

Anticancer Activity ofPhyllanthus emblicaLinn. (Indian Gooseberry): Inhibition of Transcription Factor AP-1 and HPV Gene Expression in Cervical Cancer Cells

Mahata

Pandey

Shukla

et al. 2013

Nutrition and Cancer

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Plant products of Phyllanthus emblica Linn. are traditionally consumed for its immense nutritive and medicinal values. However, the molecular mechanism(s) by which it exerts it effects is less understood. In this study, we investigated mechanism of action of P. emblica fruit extract (PE) by studying its effect on activator protein-1 (AP-1) activity and human papillomavirus (HPV) transcription that are essential for tumorigenicity of cervical cancer cells. PE resulted in a dose-and time-dependent inhibition of DNA binding activity of constitutively active AP-1 in both HPV16-positive (SiHa) and HPV18-positive (HeLa) cervical cancer cells. PE-induced AP-1 inhibition was found mediated through downregulation of constituent AP-1 proteins, c-Jun, JunB, JunD, and c-Fos; however, the kinetics of their inhibition varied in both the cell types. Inhibition of AP-1 by PE was accompanied by suppression of viral transcription that resulted in growth inhibition of cervical cancer cells. Growth inhibitory activity of PE was primarily manifested through induction of apoptotic cell death. These results suggest that P. emblica exhibits its anticancer activities through inhibition of AP-1 and targets transcription of viral oncogenes responsible for development and progression of cervical cancer thus indicating its possible utility for treatment of HPV-induced cervical cancers.

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Influence ofEmblica officinalisaqueous extract on growth and antioxidant defense system of human hepatoma cell line (HepG2)

Cited by 47 publications

References 27 publications

Aqueous extract of Terminalia arjuna attenuates tert‐butyl hydroperoxide–induced oxidative stress in HepG2 cell model

Aqueous extract of Terminalia arjuna attenuates tert‐butyl hydroperoxide–induced oxidative stress in HepG2 cell model

Emblica officinalis(Amla): A review of potential therapeutic applications

Anticancer Activity ofPhyllanthus emblicaLinn. (Indian Gooseberry): Inhibition of Transcription Factor AP-1 and HPV Gene Expression in Cervical Cancer Cells

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