Influence of IGF1, TGFβ1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

Osiecka-Iwan, Anna; Moskalewski, Stanisław; Hyc, Anna

doi:10.5603/fhc.a2016.0019

Cited by 3 publications

(3 citation statements)

References 42 publications

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“…[35] As shown in a study, cells from a preloaded bFGF group showed reformative proliferation and increased cell numbers compared with the unloaded group. [36,37] Chen and Kohno colleagues reported that bFGF is one of most vital growth factors promoting collagen protein secretion and tendon cell development. [38,39] In their research, a comparison with the control group revealed that the cells in the experimental group showed increased vessel formation, resulting in enhanced healing of the tendon-bone-interface.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of in vitro proliferation and bioactivity of human anterior cruciate ligament fibroblasts using an in situ tissue isolation method and basic fibroblast growth factor culture conditions

Liu

Ren

et al. 2019

Medicine

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Background: Previous studies have reported poor proliferation and bioactivity of human anterior cruciate ligament fibroblasts (hACLFs) after injury. As hACLFs are one of the most significant and indispensable source of seed cells in constructing tissue-engineered ligament, enhancing hACLF proliferation would offer favorable cellular-biological ability and induce the extracellular matrix secretion of hACLFs after loading on multiple types of scaffolds. Enhancing the bioactivity of hACLFs would improve tissue repair and functional recovery after tissue-engineered ligament transplantation. This study compared cells prepared by collagenase digestion and the in situ culture of tissue pieces and investigated the effect of basic fibroblast growth factor (bFGF) on hACLFs. Methods: Six adult patients participated in this study. Of these patients, tissues from three were compared after culture establishment through collagenase digestion or in situ tissue isolation. hACLF phenotypic characteristics were assessed, and the effect of bFGF on hACLF cultures was observed. hACLFs cultured with and without bFGF served as the experimental and control groups, respectively. Cell Counting Kit-8 was used to detect proliferation. The expression of ligament-related genes and proteins was evaluated by immunofluorescence staining, real-time polymerase chain reaction (PCR) assays, and Western blot assays. Results: The morphology of hACLFs isolated using the two methods differed after the 2nd passage. The proliferation of cells obtained by in situ culture was higher than that of cells obtained by collagenase digestion. hACLFs cultured with bFGF after the 3rd passage exhibited a higher proliferation rate than the controls. Immunofluorescence staining, real-time PCR, and Western blot analysis showed a significant increase in ligament-related gene and protein expression in the hACLFs cultured with bFGF. Conclusions: The in situ isolation of tissue pieces enhanced hACLF proliferation in vitro, and the hACLFs exhibited phenotypic characteristics of fibroblasts. hACLFs cultured with bFGF exhibited increased hACLF bioactivity.

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Section: Discussionmentioning

confidence: 99%

Enhancement of in vitro proliferation and bioactivity of human anterior cruciate ligament fibroblasts using an in situ tissue isolation method and basic fibroblast growth factor culture conditions

Liu

Ren

et al. 2019

Medicine

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“…Thus, TGFβ1 which is present both in CIF and CIF-like cocktail could be responsible for raise in HAS1 expression. In short, lasting 4 h only stimulation of the synovial membrane, expression of HAS1 was, however, not stimulated by TGFβ1 or IGF1 acting alone, but both factors applied together exerted distinct stimulatory effect suggestive of certain synergism (14,16). Since platelet-rich plasma (PRP), used for the treatment of joint ailments, contains, among others, TGFβ1, IGF1 and bFGF (19), the same factors which are present in CIF, administration of PRP could also stimulate HAS1 expression in crucial ligaments.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously found that CIF released from newborn rat cartilage contained bFGF, insulin-like growth factor 1 (IGF1), TGFβ1, bone morphogenetic protein 7 (BMP7), macrophage colony-stimulating factor (MCSF), granulocyte colony-stimulating factor (GCSF) and leukemia inhibitory factor (LIF). We also demonstrated that CIF stimulated a number of genes in synovial membrane and dermal fibroblasts and these effects could be partially imitated by CIF-like cocktail composed of factors identified in CIF (14)(15)(16).…”

Section: Introductionmentioning

confidence: 93%

Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts

et al. 2017

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Abstract. Loading of articular cartilage during motion squeezes the fluid from the cartilage, termed cartilage interstitial fluid (CIF), which was found to influence gene expression in synovial membrane cells. After crucial ligaments damage, these cells are exposed to synovial fluid containing factors released from articular cartilage; the aim of the present study was to establish the influence of CIF and factors present in CIF (CIF-like cocktails) on crucial ligament fibroblasts. CIF was squeezed from articular-epiphyseal cartilage complexes of newborn rats. Fibroblasts were obtained from crucial ligaments of adult rat knee joints. Cells were cultured in control medium, CIF and CIF-like cocktails, and the expression of selected genes was evaluated using quantitative PCR. CIF stimulated the expression of HAS1, HAS2, aggrecan, lubricin, MMP3, TIMP3 and TGFβ1. Expression of collagen type I, versican, MMP2, TIMP2, TNF and IL1β was inhibited. The CIF-like cocktail stimulated HAS1, HAS2, collagen type I, versican, aggrecan, lubricin, TIMP1, TGFβ1, IL1β, IL6 and inhibited of MMP3 and TNF expression. Both agents exerted similar effects on the expression of HAS2, aggrecan, lubricin, TGFβ1 and TNF. CIF contains inhibitory and stimulatory factors affecting gene expression in crucial ligament fibroblasts and some of them were not included in the CIF-like cocktail. Due to the powerful influence of CIF on crucial ligament fibroblasts and the synovial membrane, further studies on its composition are needed. An improved CIF like-cocktail could be applied in the treatment of various joint or tendon ailments.

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The Concentration of Plasma Provides Additional Bioactive Proteins in Platelet and Autologous Protein Solutions

et al. 2019

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Background: Currently, platelet-poor plasma (PPP) is a discarded waste product of platelet-rich plasma (PRP) and may contain valuable proteins. Purpose/Hypothesis: The study’s goal was to evaluate the concentration of plasma as a potential additive biotherapy for the treatment of osteoarthritis. We hypothesized that a novel polyacrylamide concentration device would efficiently concentrate insulin-like growth factor–1 (IGF-1) from PPP and be additive to PRP or autologous protein solution (APS). Study Design: Descriptive laboratory study. Methods: A laboratory study was conducted with human and equine whole blood from healthy volunteers/donors. Fresh samples of blood and plasma were processed and characterized for platelet, white blood cell, and growth factor/cytokine content and then quantified by enzyme-linked immunosorbent assays specific for IGF-1, transforming growth factor–β, interleukin-1β, and interleukin-1 receptor antagonist as representatives of cartilage anabolic and inflammatory mediators. Results: A potent cartilage anabolic protein, IGF-1, was significantly concentrated by the polyacrylamide concentration device in both human and equine PPP. The polyacrylamide device also substantially increased plasma proteins over whole blood, most dramatically key proteins relevant to the treatment of osteoarthritis, including transforming growth factor–β (29-fold over blood) and interleukin-1 receptor antagonist (70-fold over plasma). Conclusion: Concentrated PPP is a unique source for biologically relevant concentrations of IGF-1. PRP and APS can produce greater concentrations of other anabolic and anti-inflammatory proteins not found in plasma. Clinical Relevance: The polyacrylamide device efficiently concentrated PPP to create a unique source of IGF-1 that may supplement orthopaedic biologic therapies.

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Influence of IGF1, TGFβ1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

Cited by 3 publications

References 42 publications

Enhancement of in vitro proliferation and bioactivity of human anterior cruciate ligament fibroblasts using an in situ tissue isolation method and basic fibroblast growth factor culture conditions

Enhancement of in vitro proliferation and bioactivity of human anterior cruciate ligament fibroblasts using an in situ tissue isolation method and basic fibroblast growth factor culture conditions

Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts

The Concentration of Plasma Provides Additional Bioactive Proteins in Platelet and Autologous Protein Solutions

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