Influence of increasing bicarbonate concentrations on plant growth, organic acid accumulation in roots and iron uptake by barley, sorghum, and maize

Abstract

Barley, sorghum, and maize plants were grown in nutrient culture with increasing concentrations of bicarbonate supplied as NaHCO 3 (0-20 mM) and harvested at 0, 2, 4, and 8 days of treatment. Bicarbonate induced symptoms of iron (Fe) deficiency (chlorosis) in all three plant species, but sorghum and maize were more sensitive than barley. Increases in the concentration of HCO 3 -ions in the nutrient medium markedly decreased dry weight of roots and shoots in all three crop species. These effects became more distinct with time, particularly at 10 and 20 mM of bicarbonate supply. In contrast to root dry weight, however, root length was depressed by 'Present address:

Control of nutrient solutions for studies at high pH

“…Plant growth in many species is limited by excess HCO − 3 . Although the mechanisms are not fully understood, it is believed that HCO − 3 adversely effects the root plasma membrane resulting in root growth inhibition (Alhendawi et al, 1997). However, the addition of HCO − 3 in low concentrations has been observed to increase growth (Bialczyk et al, 1994;Cramer and Lips, 1995).…”

The effects of production methods and storage on the chemical constituents of apple pekmez

“…Organic acids were analyzed according to the modified methods used by Alhendawi et al (1997) and Krapez et al (2001). A Sep-Pak C18 solid-phase cartridge (Waters Associates Ireland) was equilibrated with 3 ml methanol and then washed with 10 ml ultrapure water.…”

Assessment of technological characteristics of non-fat yoghurt manufactured with prebiotics and probiotic strains

“…Sample preparations were made as described by Alhendawi et al (1997) with slight modifications. A 20 μL of the diluted solution was injected into the HPLC system (Shimadzu, Japan) equipped with a Prodigy ODS (2) (250×4.6 mm) 5 μ column.…”