Wood is increasingly being used in construction as an alternative to steel and concrete. As wood is an inhomogeneous material, this has a strong effect on its static and dynamic properties. When timber is used as a load-bearing component, there is a possibility that it will be exposed to unfavourable weather conditions (wind) or dynamic environments (vibrations), leading to fatigue of the material. In this article, the effects of load frequency and load magnitude on the durability of Norway spruce wood (Picea abies) were investigated. The frequencies of 5 and 10 Hz were compared at three load levels of 70%, 80% and 90% of the static breaking force. The research results show that the load magnitude has a major influence on the dynamic strength at the same fatigue frequency. Each increase in load means a lower dynamic strength of the spruce, which is reflected in the load cycles achieved. In addition, the dynamic properties of spruce wood deteriorate with an increasing loading frequency, which is more pronounced at higher loading forces. These test results are the basis for determining the Wöhler curve, which is required as input data for the material properties in numerical calculations to determine the durability of the material.