To cite this paper: Sattar, A., H.N. Asghar, Z.A. Zahir and M. Asghar, 2017. Bioactivation of indigenous and exogenously applied micronutrients through acidified organic amendment for improving yield and biofortification of maize in calcareous soil.
AbstractMicronutrients are required for the proper growth and development of plants, animals and human-beings. Unfortunately, owed to calcareous nature of Pakistani soils plants are suffering from severe micronutrients deficiency and to overwhelm this issue, various strategies have been adopted. Among these, elemental sulfur (Sº) is becoming a commonly practiced strategy, but, still it is accompanied by economic constraints because huge amount of Sº could not be paid by farmers. In the present study, sulfur oxidizing bacteria (SOB) were isolated and screened on the basis of pH reduction and sulphate ions production. The most efficient SOB strain was used to prepare acidified-amendment through biological-oxidation of S° amended cow-manure. After preparation and rate optimization of acidulated-amendment (liquid) in laboratory conditions, it was applied to pot grown maize (Hycorn) along with 25, 50, 75, 100% of the recommended dose of ZnSO4, FeSO4 and MnSO4; to evaluate its impact on growth, yield and bioavailability of micronutrients (Zn, Fe and Mn). Results indicated that combined use of acidulatedextract with micronutrient significantly enhanced the growth, yield and micronutrient availability of maize as compared to control. However, on the basis of economics, 50% of the recommended micronutrient + acidulated extract was found more beneficial and improved yield by 46.27% and grain Zn, Fe and Mn concentration by 42.95, 117.38 and 128.55% over control, respectively. The results may imply that acidified-product can improve the grain micronutrient contents and resultantly, humans' micronutrient deficiency would be uplifted, proving it to be a novel and eco-friendly approach for calcareous soil.