Influence of Intense Pulsed UV Irradiation on the Viability and Proliferation of HeLa Cells

Lü, Peng; Obata, Daichi; Watanabe, Tetsuya; Mitsutake, Kazunori; Abé, Keisuke; Katsuki, S.; Akiyama, H.; Zhang, C. H.

doi:10.1109/tps.2012.2201961

Cited by 5 publications

(1 citation statement)

References 29 publications

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“…where 1/τ ch is the CL reaction rate constant (induction of H2O2 and consequent reaction with NO) and τ 2 depicts the shorter chemical process time (formation of cytotoxic singlet oxygen). The 𝐼𝐼 𝑛𝑛 (t), in case with DMEM sample is fitted to Gomperz function, usually used to model biological analysis [4] Fig. 4 The chemiluminescence reaction rate constants and calculated energies from transient times of localized states extracted from measured photcurrent transients of a-Si:H p-i-n PD by samples with HeLa cells in subplot vs. time interval.…”

Section: Methodsmentioning

confidence: 99%

The Blue Light Defects Activation in A-Si:H Pin Photodiode as a Biosensor

Gradišnik

Gumbarević²

2020

KEM

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The microfluidic Lab-On-Chip (LOC) systems, based on the CMOS technology, today grow rapidly based on requirement of the Point-of-care-testing (POCT). It is a need for a high sensitive biotransducers, as a part of biosensors to be integrated on LOC system. To detect low-level of light emitted by an analyte, promising material and devices are a p-i-n a-Si:H photodiodes. The observed absorbance of blue light in human cells HeLa (cervical carcinoma) induct H2O2 in same cells and consequently, chemical reaction with NO, detected as chemiluminescence signal by the photodiode, as well as formation of cytotoxic singlet oxygen. On the other side a-Si:H p-i-n photodiode has a high sensitivity on blue light at low-light intensity, good spectral responsivity and small reflectance for blue light, low dark current, low-noise in the range of low reverse bias voltages. The photoconductivity of a-Si:H p-i-n photodiode is influenced by the native and light induced localized state density and their energy distribution in the energy gap of intrinsic a-Si:H. It is observed that the defect states of i-layer at various bias voltages contribute to the detection of HeLa cells chemiluminescence. The optical bias dependence of modulated photocurrent method (OBMPC) using the blue LED light is applied to clarify the energy gap density of state nature and energy distribution, respectively in a-Si:H p-i-n photodiode i-layer.

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Section: Methodsmentioning

confidence: 99%

The Blue Light Defects Activation in A-Si:H Pin Photodiode as a Biosensor

Gradišnik

Gumbarević²

2020

KEM

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Growth control of Saccharomyces cerevisiae through dose of oxygen atoms

Hashizume

Ohta

Hori

et al. 2015

Applied Physics Letters

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To investigate the dose-dependent effects of neutral oxygen radicals on the proliferation as well as the inactivation of microorganisms, we treated suspensions of budding yeast cells with oxygen radicals using an atmospheric-pressure oxygen radical source, varying the fluxes of O(3Pj) from 1.3 × 1016 to 2.3 × 1017 cm−2 s−1. Proliferation was promoted at doses of O(3Pj) ranging from 6 × 1016 to 2 × 1017 cm−3, and suppressed at doses ranging from 3 × 1017 to 1 × 1018 cm−3; cells were inactivated by O(3Pj) doses exceeding 1 × 1018 cm−3, even when the flux was varied over the above flux range. These results showed that the growth of cells was regulated primarily in response to the total dose of O(3Pj).

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