Influence of Internal and External Factors on the Production of Cytokines by Peripheral Blood Cells in Breast Cancer

Autenshlyus, A. I.; Davletova, K I; Mikhaylova, E. S.; Проскура, А В; Вараксин, Н. А.; Bogachuk, A. P.; Sidorov, S. V.; Lyakhovich, V. V.; Липкин, В. М.

doi:10.1134/s1607672920040031

Cited by 2 publications

(5 citation statements)

References 9 publications

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“…The downstream IL-1β was correlated with large tumor size, clinical stage, histological grade [ 70 , 72 ], endocrine- and chemo-resistance in HR+ BC patients [ 75 , 76 , 77 ]. Moreover, IL-1β production by peripheral blood cells enhanced lymphatic metastasis in BC [ 69 ]. Recently, sPD-L1 has attracted much attention [ 85 ] since its high serum levels were correlated with metastasis, immunosuppression, and poor prognosis in TNBC patients [ 87 , 88 ].…”

Section: Discussionmentioning

confidence: 99%

“…In BC, high levels of IL-1β showed various tumor-promoting impacts [ 144 , 145 , 146 ]. Peripheral blood cells of invasive BC patients produced high levels of IL-1β, leading to metastasis [ 69 ]. In sera and plasma of BC patients, ELISA findings showed that IL-β expressions were significantly increased compared to those in the control group [ 70 , 71 ].…”

Section: Discussionmentioning

confidence: 99%

“…Distinct studies detected its expression in PBMCs [ 65 , 66 , 67 , 68 ]. In patients with invasive BC, IL-1β production by peripheral blood cells was associated with lymphatic metastasis [ 69 ]. Enzyme-linked immunosorbent assay (ELISA) findings showed that IL-β expressions were significantly increased in the sera and plasma of BC patients compared to the control group [ 70 , 71 ] and were related to tumor size, clinical stage, histological grade, and lymph node metastasis [ 70 ].…”

Section: Introductionmentioning

confidence: 99%

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Thymoquinone, a Novel Multi-Strike Inhibitor of Pro-Tumorigenic Breast Cancer (BC) Markers: CALR, NLRP3 Pathway and sPD-L1 in PBMCs of HR+ and TNBC Patients

Elgohary,

Eissa,

El Tayebi

2023

IJMS

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Breast cancer (BC) is not only a mass of malignant cells but also a systemic inflammatory disease. BC pro-tumorigenic inflammation has been shown to promote immune evasion and provoke BC progression. The NOD-like receptor (NLR) family pyrin domain-containing protein 3 (NLRP3) inflammasome is activated when pattern recognition receptors (PRRs) sense danger signals such as calreticulin (CALR) from damaged/dying cells, leading to the secretion of interleukin-1β (IL-1β). CALR is a novel BC biological marker, and its high levels are associated with advanced tumors. NLRP3 expression is strongly correlated with an elevated proliferative index Ki67, BC progression, metastasis, and recurrence in patients with hormone receptor-positive (HR+) and triple-negative BC (TNBC). Tumor-associated macrophages (TAMs) secrete high levels of IL-1β promoting endocrine resistance in HR+ BC. Recently, an immunosuppressive soluble form of programmed death ligand 1 (sPD-L1) has been identified as a novel prognostic biomarker in triple-negative breast cancer (TNBC) patients. Interestingly, IL-1β induces sPD-L1 release. BC Patients with elevated IL-1β and sPD-L1 levels show significantly short progression-free survival. For the first time, this study aims to investigate the inhibitory impact of thymoquinone (TQ) on CALR, the NLRP3 pathway and sPD-L1 in HR+ and TNBC. Blood samples were collected from 45 patients with BC. The effect of differing TQ concentrations for different durations on the expression of CALR, NLRP3 complex components and IL-1β as well as the protein levels of sPD-L1 and IL-1β were investigated in the peripheral blood mononuclear cells (PBMCs) and TAMs of TNBC and HR+ BC patients, respectively. The findings showed that TQ significantly downregulated the expression of CALR, NLRP3 components and IL-1β together with the protein levels of secreted IL-1β and sPD-L1. The current findings demonstrated novel immunomodulatory effects of TQ, highlighting its potential role not only as an excellent adjuvant but also as a possible immunotherapeutic agent in HR+ and TNBC patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thymoquinone, a Novel Multi-Strike Inhibitor of Pro-Tumorigenic Breast Cancer (BC) Markers: CALR, NLRP3 Pathway and sPD-L1 in PBMCs of HR+ and TNBC Patients

Elgohary,

Eissa,

El Tayebi

2023

IJMS

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“…Th-2 регулируют иммунные ответы, продуцируя противовоспалительные цитокины: IL-4, IL-5, IL-9 и IL-13. Кроме того, IL-10 может участвовать в предотвращении альтерации тканей [24][25][26].…”

Section: результаты и обсуждениеunclassified

“…Фактически выявленные дисбалансы являются манифестными формами сердечно-сосудистых, бронхолёгочных, мочеполовых и других заболеваний [26][27][28]. Совокупность выявленных иммунных дисбалансов как манифестных форм групп заболеваний у 16-88 лиц настораживает и требует дополнительного исследования в целях профилактики развития сердечно-сосудистой, мочеполовой и других патологий у обследуемых женщин на Севере.…”

Section: показателиunclassified

Cellular immunity in women aged 20–40 living in different climatic and geographic regions

Shchegoleva,

Shashkova,

Sergeeva

2024

Acta biomedica scientifica

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The aim. To identify phenotypic features of adaptive cellular immune responses in young women living in different climatic and geographic regions.Materials and methods. We examined 63 apparently healthy women, including 25 residents of Sovpolye settlement, Arkhangelsk region (Arctic region) and 38 residents of Tskhinvali (Republic of South Ossetia) aged 20–40. The content of lymphocytes, their phenotypes CD4+, CD8+, CD3+, CD5+, CD16+, CD10+, CD71+, CD25+, HLA-DR+ and CD95+, CEA (carcinoembryonic antigen) glycoprotein, and interleukin (IL) 6 and IL-10 cytokines was studied. Lymphocytes phenotyping was performed by indirect immunoperoxidase reaction using monoclonal antibodies (MedBioSpektr, Moscow) on “dried drop” lymphocyte sample with peroxidase conjugate and chromogen solution staining for immersion microscopy analysis (Nicon 50i, Japan). The content of CEA glycoprotein and cytokines was determined using ELISA.Results. In women living in Arctic region, a deficiency of CD5+, CD3+, CD10+, CD95+, CD71+, CD25+, HLA-DR+ cells, CEA and IL-6 concentrations is associated with high concentrations of CD8+ and IL-10. In women living in Southern region, a slight deficiency of CD5+, CD8+ and CD95+ cells is associated with high concentrations of CEA, IL-6, IL-10 and CD10+, CD16+ and HLA-DR+ cells.Conclusion. In inhabitants of different climatic regions, the features of formation of adaptive immune reactions are determined by different quantitative and qualitative composition of lymphocytes and cytokines phenotypes. Reduction of reserve capabilities of immune homeostasis is detected 3 times more often in inhabitants of the Arctic region.

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Influence of Internal and External Factors on the Production of Cytokines by Peripheral Blood Cells in Breast Cancer

Cited by 2 publications

References 9 publications

Thymoquinone, a Novel Multi-Strike Inhibitor of Pro-Tumorigenic Breast Cancer (BC) Markers: CALR, NLRP3 Pathway and sPD-L1 in PBMCs of HR+ and TNBC Patients

Thymoquinone, a Novel Multi-Strike Inhibitor of Pro-Tumorigenic Breast Cancer (BC) Markers: CALR, NLRP3 Pathway and sPD-L1 in PBMCs of HR+ and TNBC Patients

Cellular immunity in women aged 20–40 living in different climatic and geographic regions

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