Influence of Intestinal Microflora on Murine Bone Marrow and Spleen Macrophage Precursors

Nicaise, Pascale; Gleizes, Aude; Sandré, Catherine; Forestier, F; Kergot, R; Quero, A.M.; Labarre, C

doi:10.1046/j.1365-3083.1998.00487.x

Cited by 26 publications

(20 citation statements)

References 23 publications

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“…Additional purifications using CD4 and CD8 antibodies and a Cytopeia Influx cell sorter (BD Bioscience) achieved >99% CD4 and CD8 purities. BM cells were isolated from the femurs and cultured in DMEM supplemented with L929 conditioned media for 7 days as described [36]. Purity of the BM derived macrophage (BMDM) populations were >90% macrophages.…”

Section: Methodsmentioning

confidence: 99%

Murine CD8+ T cells but not macrophages express the vitamin D 1α-hydroxylase

Ooi

McDaniel

Weaver

et al. 2014

The Journal of Nutritional Biochemistry

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The active form of vitamin D, 1,25-dihydroxyvitamin D [1,25(OH)2D3] is synthesized by the 1α-hydroxylase, which is encoded by the Cyp27B1 gene. Using transgenic mice that have replaced the Cyp27B1 gene with the bacterial lacZ reporter gene (β-galactosidase), the inflammatory conditions that induce Cyp27B1 in the immune system were probed. A variety of stimuli including lipopolysaccharide, anti-CD3 or PMA/ionomycin were used to stimulate splenocytes and bone marrow derived macrophage in vitro. Only anti-CD3 stimulation resulted in a low induction of β-galactosidase activity in the spleen, indicating that T cells might be a source of Cyp27B1. In vivo, challenge with lipopolysaccharide, α-galactosylceramide, and Listeria monocytogenes failed to induce β-galactosidase activity outside of the kidneys. During more prolonged and severe inflammation there was staining in both the lungs and the gastrointestinal tract for β-galactosidase. Furthermore, wild type reconstitution of the hematopoietic cell population in Cyp27B1 KO mice protected the mice from experimental colitis. T cell production of Cyp27B1 activity was shown to be from the CD8+ but not the CD4+ T cell population. CD8+ T cells expressed the reporter gene only after 48h of stimulation. The data is consistent with a model where CD8+ T cells are activated to produce Cyp27B1 and 1,25(OH)2D3 that serves to turn off the local immune response.

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Section: Methodsmentioning

confidence: 99%

Murine CD8+ T cells but not macrophages express the vitamin D 1α-hydroxylase

Ooi

McDaniel

Weaver

et al. 2014

The Journal of Nutritional Biochemistry

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“…Likewise, a recent study has shown that GF Swiss mice developed progressive lesions when infected with L. major, whereas SPF Swiss mice only make small lesions and eventually heal (29). Lastly, macrophages derived from the spleen of GF mice expressed reduced amounts of MHC class II molecules (30) and exhibited impaired ability to secrete IL-1, IL-6, and IL-12 (31)(32)(33). Thus, although T cells from GF mice differ from those from SPF mice because they have not been exposed to gut flora Ags, GF mice exhibit many other defects that could explain why they remained susceptible to L. major despite the lack of the parasiteinduced IL-4 burst.…”

Section: Cd62lmentioning

confidence: 99%

Priming by Microbial Antigens from the Intestinal Flora Determines the Ability of CD4+ T Cells to Rapidly Secrete IL-4 in BALB/c Mice Infected withLeishmania major

Julia

McSorley

Malherbe

et al. 2000

The Journal of Immunology

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Infection of BALB/c mice with Leishmania major results in the rapid accumulation of IL-4 transcripts within CD4+ T cells that react to the parasite Leishmania homologue of mammalian RACK1 (LACK) Ag. Because memory/effector cells secrete IL-4 more rapidly than naive cells, we sought to analyze the phenotype of these lymphocytes before infection. Indeed, a fraction of LACK-specific CD4+ T cells expressed a typical CD62 ligandlowCD44highCD45RBlow phenotype in uninfected mice. LACK-specific T cells were primed in gut-associated lymphoid tissues by cross-reactive microbial Ags as demonstrated by their reactivity with bacterial extracts and by the ability of APCs from the mesenteric LN of BALB/c mice to induce their proliferation. Also, mice in which the digestive tract has been decontaminated exhibited a reduced proportion of LACK-specific T cells expressing a memory/effector phenotype and did not exhibit the early accumulation of IL-4 transcripts induced by L. major. Thus, LACK-specific T cells represent a subset of CD4+ T cells which have acquired the ability to rapidly secrete IL-4 as the result of their priming by cross-reactive microbial Ags. Tracking the fate of these cells may provide information about the regulation of cell-mediated immune responses to gut Ags in physiological and pathological situations.

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“…However, the expression of MHC class II remains lower in GF mice. Thus, ora seems to enhance this antigen-presenting molecule preferentially in a secondary lymphoid organ (8).…”

Section: Effects Of Indigenous Micro Oramentioning

confidence: 90%

“…These results suggest that macrophage generation is more dependent on ora in the splenic than in the medullary environment. (8).…”

Section: Effects Of Indigenous Micro Oramentioning

confidence: 99%

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Influence of Microbial Flora on Macrophages

Forestier¹

2000

Microbial Ecology in Health and Disease

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The purpose of this article was to collect the existing data on the in uence of bacterial ora on the properties of macrophages which represent one of the rst lines of non speci c defence against microbial invasion and tumours. To the effects of indigenous micro ora were added those of lactic acid bacteria potentially usable as dietary supplements which have been widely investigated. The results of these studies indicate that functions important in host defence, such as chemotaxis, phagocytosis, cytotoxicity, intracellular killing of bacteria as well as cytokine production, appear to be, at least in part, under the control of the indigenous micro ora. Though lactic acid bacteria exert stimulating effects on macrophage functions, their impact is less clearly established and seems to be strain-and test system-dependent. Results showing macrophage activation in humans by orally administered lactic bacteria are very limited and more studies are needed to correlate the stimulation of macrophage functions and positive health effects of these probiotic products.

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Influence of Intestinal Microflora on Murine Bone Marrow and Spleen Macrophage Precursors

Cited by 26 publications

References 23 publications

Murine CD8+ T cells but not macrophages express the vitamin D 1α-hydroxylase

Murine CD8+ T cells but not macrophages express the vitamin D 1α-hydroxylase

Priming by Microbial Antigens from the Intestinal Flora Determines the Ability of CD4+ T Cells to Rapidly Secrete IL-4 in BALB/c Mice Infected withLeishmania major

Influence of Microbial Flora on Macrophages

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