Influence of intestinal resections on biliary composition and liver ultrastructure

Antúnez, M.I. Correa; Penco, J M Morán; Lozano, J.; Macho, A; Climent, Vicente

doi:10.1016/j.clnu.2010.09.002

Cited by 13 publications

(1 citation statement)

References 11 publications

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“…TEM studies were performed according to the protocol as previously described (Antúnez et al, 2011). After collection of spleen tissue, they were processed and viewed under TEM Zeiss 900 (Zeiss, Germany).…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Cell apoptosis of caprine spleen induced by toxicity of cadmium with different levels of molybdenum

Chen

et al. 2015

Environmental Toxicology and Pharmacology

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Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Cell apoptosis of caprine spleen induced by toxicity of cadmium with different levels of molybdenum

Chen

et al. 2015

Environmental Toxicology and Pharmacology

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In Vivo Studies of Molybdenum-Induced Apoptosis in Kidney Cells of Caprine

Ali

Chen

et al. 2015

Biol Trace Elem Res

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Molybdenum (Mo) is an essential microelement for the health of animals and human beings, and high dietary intake of Mo can lead to pathological conditions. However, the cytotoxic effects of high levels of Mo on the renal cells in ruminants have not been reported. Therefore, this in vivo study in goats was designed to investigate the impact of Mo on kidney-related apoptosis genes, and histopathological and ultrastructural changes in renal cells using real-time quantitative polymerase chain reaction (RT-qPCR), light microscopy, and transmission electron microscopy. A total of 48 goats were randomly distributed in equal number into four groups and assigned with one of three oral treatments of ammonium molybdate (15, 30, and 45 mg Mo kg(-1) BW), while control group received no Mo. Kidney tissues were taken from individual goat at days 0, 25, and 50 for determining expression of apoptosis genes including Bax, Bcl-2, Cyt c, caspase-3, and Smac. The results revealed that the expression of Bax, Smac, Cyt c, and caspase-3 was significantly (P < 0.05 or P < 0.01) upregulated in renal cells, whereas Bcl-2 was downregulated (P < 0.01). Histopathological lesions showed degeneration of renal tubule, glomerular atrophy, and dilation of Bowman's capsule. In addition, ultrastructural injury that predicted varying degrees of vacuolization, irregularity, fission of the nucleus, and swelling of mitochondria was observed in the cytoplasma of cells in groups treated with 30 and 45 mg Mo kg(-1). This concluded that high levels of molybdenum, which induces apoptosis of caprine renal cells, might be involved in the mitochondrial intrinsic pathway.

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Varying Dietary Levels of Molybdenum Inducing Cell Apoptosis of Spleen Under Cadmium Stress in Caprine

Xiao

Zhang

et al. 2015

Biol Trace Elem Res

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The present experiment aims at evaluating chronic toxic effects of the combination of cadmium (Cd) and molybdenum (Mo) according to residual element contents, apoptosis gene expression, and ultrastructure and histopathology changes of caprine spleen. In total, 36 Boer goats were randomly divided into four groups with the equal number in each group. The control group was orally administered with deionized water while the experimental groups I, II, and III were administered with the equal quantity of CdCl2 (1 mg kg(-1) BW) and (NH4)6·Mo7O24·4H2O including 15, 30, and 45 mg·Mo kg(-1) BW, respectively. Three individuals from each group were treated with euthanasia on days 0, 25, and 50. The data showed that the content of splenic residual Mo and Cd increased (P < 0.05) in the experimental groups on days 25 and 50, while no significant difference was observed in the content of Cu. The apoptosis-related gene expression levels including Bcl-2, Bax, Caspase-3, Smac, and ceruloplasmin (CP) were also determined. Results showed that significant reductions were observed in Bcl-2 and CP expressions (P < 0.01), while Caspase-3 gene was up-regulated (P < 0.05). However, no significant difference was observed in Smac and Bax expressions. Furthermore, on day 50, spleen tissues were presented to observe ultrastructural changes in lesions by means of transmission electron microscopy, with fragmentized nucleus, vesiculation of cytoplasm, mitochondria hyperplasia, and increasing lysosomes included. In addition, histopathology results corroborated the toxicity by showing cell hemorrhage, thickening central arteries, and enhanced capsule thickness. To sum up, our study revealed that the combination of Cd and Mo could induce remarkable damage to the spleen of goats by promoting cell apoptosis in the mitochondrial pathway and affecting the deposition of Mo and Cd.

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Influence of intestinal resections on biliary composition and liver ultrastructure

Cited by 13 publications

References 11 publications

Cell apoptosis of caprine spleen induced by toxicity of cadmium with different levels of molybdenum

Cell apoptosis of caprine spleen induced by toxicity of cadmium with different levels of molybdenum

In Vivo Studies of Molybdenum-Induced Apoptosis in Kidney Cells of Caprine

Varying Dietary Levels of Molybdenum Inducing Cell Apoptosis of Spleen Under Cadmium Stress in Caprine

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