Influence of iron supplementation frequency on absorption efficiency and mucosal ferritin in anaemic rats

Benito, Paloma; House, William A.; Miller, Dennis D.

doi:10.1079/bjn19970164

Cited by 8 publications

(7 citation statements)

References 16 publications

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“…The primary strategies for correcting Fe deficiency in populations are dietary modification or diversification to improve Fe intake and bioavailability; Fe supplementation and Fe fortification of foods; and biofortification by plant breeding ( 31 ) . Although the efficiency of Fe absorption increases as Fe stores become depleted, the amount absorbed from foods, especially when diets are low in meat, fish, fruit and vegetables, is not enough to prevent Fe deficiency in many women and children, especially in developing countries ( 31 , 32 ) . For instance, the primary cause of increasing Fe deficiency in Brazil is that the consumption of food items considered to be Fe sources has continually decreased ( 33 ) .…”

Section: Discussionmentioning

confidence: 99%

Fructo-oligosaccharides and iron bioavailability in anaemic rats: the effects on iron species distribution, ferroportin-1 expression, crypt bifurcation and crypt cell proliferation in the caecum

et al. 2014

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The present study investigated the effects of fructo-oligosaccharides (FOS) on the bioavailability of Fe from ferric pyrophosphate (FP), a water-insoluble compound, in Fe-deficient anaemic rats that were subjected to a Hb repletion assay. Male Wistar rats (n 64) were fed adequate or low (8 mg/kg) Fe diets for 15 d followed by 1 or 2 weeks of Fe repletion with diets providing 35 mg Fe/kg as ferrous sulphate (FS), FP or FP that was mixed with 7·5 % FOS in the form of yacon flour or Raftilose P95 (RAF), a purified source of FOS. The effects of FOS were observed within the 1st week of the repletion period. Fe bioavailability was improved by FOS supplementation, as measured by Hb regeneration efficiency and hepatic Fe stores, which were more pronounced in the RAF group. Moreover, RAF supplementation resulted in a higher biological value relative to that of the FP group. FOS supplementation resulted in caecal enlargement, in addition to acidification and Fe species redistribution in the caecal contents relative to the control rats. These effects occurred concomitantly with decreased ferroportin (FPN)-1 expression in the caecal mucosa, which was similar in magnitude to that observed in the FS group. Caecum mucosal morphometry was influenced by FOS supplementation, whereas crypt fission and cell proliferation were highest in the caecum of the RAF group. These results reinforce the effects of FOS as Fe bioavailability enhancers in anaemic rats that are sustained by early changes in their caecal environment (decreased mucosal FPN-1 expression and increased Fe absorbability, crypt fission and cellularity).

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Section: Discussionmentioning

confidence: 99%

Fructo-oligosaccharides and iron bioavailability in anaemic rats: the effects on iron species distribution, ferroportin-1 expression, crypt bifurcation and crypt cell proliferation in the caecum

et al. 2014

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“…The rats initially weighed about 70g each and had free access to a low-Fe diet for the first 4d. They were then trained to meal-feed as described previously (Benito et al 1997). Two meals were offered daily: one at 06.00 and one at 18.00 hours.…”

Section: Animals and Dietsmentioning

confidence: 99%

“…Development of anaemia was monitored by measuring haemoglobin (Hb) concentrations in six randomly selected animals. Blood samples were collected as described previously (Benito et al 1997). Mild anaemia (90g Hb/l) became apparent after the rats were fed on the low-Fe diet for 12 d. Subsequently, Hb concentrations were determined in all animals and the rats were assigned to treatment groups.…”

Section: Animals and Dietsmentioning

confidence: 99%

Comparison of oral and intraperitoneal iron supplementation in anaemic rats: a re-evaluation of the mucosal block theory of iron absorption

Benito

House²,

Miller

1998

Br J Nutr

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To evaluate the extent to which daily oral Fe supplements may block Fe absorption from a subsequent dose, we compared effects of oral and intraperitoneal (i.p.) Fe supplementation on Fe status in anaemic rats (haemoglobin (Hb) 90 g/l). A ligated duodenal loop technique was used to assess the effects of the Fe supplements administered either orally or i.p. at different frequencies on Fe absorption from a subsequent test dose. Anaemic Sprague-Dawley rats were assigned to seven groups of eight rats each and received either oral or i.p. Fe supplements for 3 d as follows: (1) 4 mg oral supplement daily (three doses in 3 d); (2) 4 mg oral supplement once (one dose on day 1, low-Fe dose on days 2 and 3); (3) 12 mg oral supplement once (one dose on day 1, low-Fe dose on days 2 and 3); (4) 3.2 mg i.p. supplement daily (three doses in 3 d); (5) 3.2 mg i.p. supplement once (one dose on day 1); (6) 9.6 mg i.p. supplement once (one dose on day 1); (7) low-Fe diet (control). The effectiveness of the supplements in treating Fe deficiency on each of the two test-factors, i.e. route of administration and frequency of dose, was assessed by determining Hb-Fe gain and liver-Fe stores after the 3 d test period. Oral supplementation was as effective as i.p. in improving the Fe status of the anaemic animals. However, a 15 min absorption of a radio-Fe test dose from a ligated loop in i.p.-supplemented groups was significantly higher (11.68 (SD 1.70)%, 17.49 (SD 4.59)%, 16.71 (SD 3.39)%) than in orally supplemented groups (3.24 (SD 1.35)%, 2.45 (SD 1.05)%, 1.80 (SD 0.35)%) despite equal body Fe stores. No significant difference in intestinal Fe absorption efficiency was detected within the oral groups but those supplemented only once were more effective than or as effective as the group receiving daily supplements for 3 d in improving Fe status as indicated by Hb-regeneration efficiency. We conclude that there is a mucosal block with the administration of oral Fe supplements but the extent of this blocking effect during oral Fe supplementation is not as dramatic as currently thought in the context of the poor efficacy of daily Fe supplementation programmes.

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“…9,10 In this study, the absorption rate and tissue uptake of Fe 59 were similar to those noted in recent reports. 19 Our findings showed that absorption, blood content, and spleen uptake of ␤-CN (1-25)-bound iron by duodenal rat loop during the experiment were better than that of inorganic iron. The better absorption and tissue uptake of ␤-CN (1-25) bound iron supports the results of tissue analysis after a 4-week repletion period.…”

Section: Discussionmentioning

confidence: 52%

Bioavailability of caseinophosphopeptide-bound iron

Aît-Oukhatar

Pérès²,

Bouhallab³

et al. 2002

Journal of Laboratory and Clinical Medicine

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Iron deficiency, one of the main worldwide nutritional deficiencies, results from the low bioavailability of most dietary iron, including cow milk. Hydrolysis of the cow milk protein casein produces low molecular weight caseinophosphopeptides (CPPs). Binding of iron to CPPs keeps it soluble in the digestive tract and prevents the formation of high molecular weight ferric hydroxides, which are poorly absorbed. Previous experimental studies have shown that iron bound to the phosphopeptide containing the first 25 amino acids of beta-casein, or beta-CN (1-25), is well absorbed and corrects efficiently iron deficiency. We sought to assess in vivo iron absorption and uptake by tissues involved in iron metabolism and storage (liver, spleen, bone marrow), using radiolabeled iron. beta-CN (1-25)-Fe displayed better absorption and tissue uptake by the vascularized rat loop model compared with a control substance, ferric ascorbate. The metabolism of beta-CN (1-25)-Fe labeled with iron 59, added to cow milk, was also studied in young women. Although the absorption of beta-CN (1-25)-Fe was not significantly higher than that of ferrous sulfate, it displayed significantly higher tissue uptake. This increase was transient and had disappeared by the 14th day of the study, suggesting that iron was used for metabolic purposes.

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Influence of iron supplementation frequency on absorption efficiency and mucosal ferritin in anaemic rats

Cited by 8 publications

References 16 publications

Fructo-oligosaccharides and iron bioavailability in anaemic rats: the effects on iron species distribution, ferroportin-1 expression, crypt bifurcation and crypt cell proliferation in the caecum

Fructo-oligosaccharides and iron bioavailability in anaemic rats: the effects on iron species distribution, ferroportin-1 expression, crypt bifurcation and crypt cell proliferation in the caecum

Comparison of oral and intraperitoneal iron supplementation in anaemic rats: a re-evaluation of the mucosal block theory of iron absorption

Bioavailability of caseinophosphopeptide-bound iron

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