Influence of isobutylmethylxanthine on the direct current electroretinogram of albino rabbit eyes

Jarkman, Sven

doi:10.1007/bf00155034

Doc Ophthalmol

1989

DOI: 10.1007/bf00155034

|View full text |Cite

Influence of isobutylmethylxanthine on the direct current electroretinogram of albino rabbit eyes

Sven Jarkman

Abstract: The direct current electroretinogram and the standing potential of the eye were recorded from both eyes of unilaterally vitrectomized albino rabbits. The effect of intraocular irrigation with the nonselective phosphodiesterase inhibitor isobutylmethylxanthine (IBMX) was compared with that of a balanced salt control solution. IBMX (0.5 mM) produced a reversible increase in the amplitudes of the a-wave (19%, p less than 0.02), b-wave (21%, p less than 0.001) and c-wave (12%, p less than 0.01) of the electroretin… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1995

1997

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

A cyclic adenosine monophosphate agonist elevates the b- and c-waves of the rabbit direct-current electroretinogram

Bragadóttir

Jarkman

1995

Doc Ophthalmol

View full text Add to dashboard Cite

The effects of the stable cyclic adenosine monophosphate analogue adenosine 3',5'-cyclic monophosphorothioate Sp-isomer (Sp-cAMPS) on the direct-current electroretinogram and the standing potential of the eye were studied. Corneal recordings were obtained from unilaterally vitrectomized albino rabbit eyes during alternating intravitreal perfusions with Sp-cAMPS and a control solution (Pharmacia eye irrigating solution). The contralateral eye was used as a control. To evaluate further the effects on the c-wave, in vivo intraretinal microelectrode measurements were made during simultaneous intravitreal perfusion of Sp-cAMPS and irrigating solution, respectively. Sp-cAMPS in concentrations of 1, 10 and 100 microM was tested by corneal direct-current electroretinography. There was no significant effect on the a-wave amplitude. The b-wave amplitude was reversibly elevated at an Sp-cAMPS concentration of 100 microM was tested by corneal direct-current electroretinography. There was no significant effect on the a-wave amplitude. The b-wave amplitude was reversibly elevated at an Sp-cAMPS concentration of 100 microM (p < 0.01, n = 7). The c-wave amplitude was reversibly elevated at an Sp-cAMPS concentration of 100 microM (p < 0.01, n = 7). The c-wave amplitude was reversibly elevated at a concentration of 10 microM (p < 0.001, n = 8), and this effect was more pronounced at 100 microM (p < 0.001, n = 7). The SP increased reversibly at a concentration of 100 microM (p < 0.001, n = 7). Microelectrode recordings were performed with Sp-cAMPS at a concentration of 100 microM. The recordings showed significant increases in both the transepithelial potential (p < 0.01, n = 3) and the slow PIII (p < 0.01, n = 3). The effects of Sp-cAMPS on the b-wave as well as the two components of the c-wave suggest influences on both the inner retina and the retinal pigment epithelium of the rabbit eye.

show abstract

A cyclic adenosine monophosphate agonist elevates the b- and c-waves of the rabbit direct-current electroretinogram

Bragadóttir

Jarkman

1995

Doc Ophthalmol

View full text Add to dashboard Cite

show abstract

Serotonin elevates the c-wave of the electroretinogram of the rabbit eye by increasing the transepithelial potential

1997

View full text Add to dashboard Cite

The influence of serotonin (5-hydroxytryptamine, 5-HT) and serotonin analogues on the direct current electroretinogram (d.c. ERG) and the standing potential of the albino rabbit eye (SP) was studied. After unilateral vitrectomy, corneal recordings were obtained during simultaneous intravitreal perfusion with a control solution alternating with 5-HT at concentrations of 25, 120 and 200 microM. The c-wave increased at 25 and 120 microM when changing from control solution to test solution (P < 0.05) but did not decrease significantly when changing back to control solution (P > 0.05). The c-wave was reversibly elevated at 200 microM (PHS-5-HT, P < 0.01; 5-HT-PHS, P < 0.05). To analyse further the influence on the c-wave, in vivo intraretinal microelectrode recordings were obtained during intravitreal perfusion with 5-HT. The transepithelial potential (TEP) increased (P < 0.01), while the slow PIII was not significantly affected (P > 0.05). The serotonin receptor agonists 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane, 5-methoxytryptamine, alpha-methyl-5-hydroxytryptamine and 2-methyl-5-hydroxytryptamine, caused a significant reversible elevation of the c-wave, whereas 5-carboxyamidotryptamine did not. Tropisetron did not block the serotonin effect and LY53857 had an effect of its own on the c-wave. The results seem to indicate that the influence of serotonin on the c-wave is mainly due to an effect on the retinal pigment epithelium (RPE) and that more than one type of serotonin receptor may be involved.

show abstract

Effects of PhXA41, a prostaglandin analogue, and PGF_2αon the corneal and intraretinal d.c. electroretinogram (ERG) of the albino rabbit eye

Bragadóttir

Jarkman

1995

Current Eye Research

View full text Add to dashboard Cite

PhXA41, a prostaglandin F2 alpha analogue, is a new drug alternative for medical treatment of glaucoma. From earlier studies, it is known that certain prostaglandins (pgs) exert an influence on the d.c. electroretinogram (ERG). In the present study, possible effects of intraocular perfusion with PhXA41 (free acid) or PGF2 alpha on the rabbit retina and retinal pigment epithelium (RPE) were evaluated. Unilateral vitrectomy was performed and a continuous intraocular perfusion was established, in which a reference solution was alternated with the test solution. In part one of the experiments, the corneal d.c. ERG and the standing potential of the eye (SP) were recorded from both eyes. In part two, vitreal and intraretinal d.c. ERG were recorded in the vitrectomized eye. PhXA41 and PGF2 alpha produced similar effects, i.e. a significant and reversible c-wave increase at concentrations of 1-10 micrograms/ml. No influence was found in the concentration interval 10-100 ng/ml. There were no changes in a- and b-wave amplitudes at any of the concentrations tested. At a concentration of 10 micrograms/ml, the SP increased significantly. The intraretinal d.c. ERG showed a large reduction of slow PIII accompanied by a small change in transepithelial potential (TEP). Theoretically, a vitreous concentration of less than 1 ng/ml may be reached after topical application. This concentration level does not influence the d.c. ERG in this animal model. At higher concentrations, a significant and reversible c-wave increase seemed to be generated mainly in the inner retina by a reduction of slow PIII generated in the Müller cells. The effect of PhXA41 appears to be mediated by an FP receptor.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Influence of isobutylmethylxanthine on the direct current electroretinogram of albino rabbit eyes

Cited by 3 publications

References 11 publications

A cyclic adenosine monophosphate agonist elevates the b- and c-waves of the rabbit direct-current electroretinogram

A cyclic adenosine monophosphate agonist elevates the b- and c-waves of the rabbit direct-current electroretinogram

Serotonin elevates the c-wave of the electroretinogram of the rabbit eye by increasing the transepithelial potential

Effects of PhXA41, a prostaglandin analogue, and PGF_2αon the corneal and intraretinal d.c. electroretinogram (ERG) of the albino rabbit eye

Contact Info

Product

Resources

About

Influence of isobutylmethylxanthine on the direct current electroretinogram of albino rabbit eyes

Cited by 3 publications

References 11 publications

A cyclic adenosine monophosphate agonist elevates the b- and c-waves of the rabbit direct-current electroretinogram

A cyclic adenosine monophosphate agonist elevates the b- and c-waves of the rabbit direct-current electroretinogram

Serotonin elevates the c-wave of the electroretinogram of the rabbit eye by increasing the transepithelial potential

Effects of PhXA41, a prostaglandin analogue, and PGF2αon the corneal and intraretinal d.c. electroretinogram (ERG) of the albino rabbit eye

Contact Info

Product

Resources

About

Effects of PhXA41, a prostaglandin analogue, and PGF_2αon the corneal and intraretinal d.c. electroretinogram (ERG) of the albino rabbit eye