Influence of kaempferol, a flavonoid compound, on membrane-bound ATPases in streptozotocin-induced diabetic rats

Abstract

Context: Kaempferol is a flavonoid found in many edible plants (e.g. tea, cabbage, beans, tomato, strawberries, and grapes) and in plants or botanical products commonly used in traditional medicine. Numerous preclinical studies have shown that kaempferol have a wide range of pharmacological activities, including antioxidant, anti-inflammatory, anticancer, cardioprotective, neuroprotective, and antidiabetic activities. Objective: The present study investigates the effect of kaempferol on membrane-bound ATPases in erythrocytes and in liver, kidney, and heart of streptozotocin (STZ)-induced diabetic rats. Materials and methods: Diabetes was induced into adult male albino rats of the Wistar strain, by intraperitoneal administration of STZ (40 mg/kg body weight (BW)). Kaempferol (100 mg/kg BW) or glibenclamide (600 mg/kg BW) was administered orally once daily for 45 d to normal and STZinduced diabetic rats. The effects of kaempferol on membrane-bound ATPases (total ATPase, Na + /K + -ATPase, Ca 2+ -ATPase, and Mg 2+ -ATPase) activity in erythrocytes and in liver, kidney, and heart were determined. Results: In our study, diabetic rats had significantly (p50.05) decreased activities of total ATPases, Na + /K + -ATPase, Ca 2+ -ATPase, and Mg 2+ -ATPase in erythrocytes and tissues. Oral administration of kaempferol (100 mg/kg BW) or glibenclamide (600 mg/kg BW) for a period of 45 d resulted in significant (p50.05) reversal of these enzymes' activities to near normal in erythrocytes and tissues when compared with diabetic control rats. Discussion and conclusion: Thus, obtained results indicate that administration of kaempferol has the potential to restore deranged activity of membrane-bound ATPases in STZ-induced diabetic rats. Further detailed investigation is necessary to discover kaempferol's action mechanism.

Molecular Pathways Involved in the Amelioration of Myocardial Injury in Diabetic Rats by Kaempferol

“…Thus, the mechanism by which kaempferol improved cardiac function may be related to RAGE mediated oxidative stress suppression. This is in accordance with other studies which have established its antioxidant effect in various disease models [16,33,34]. …”

“…An in vitro study has reported that kaempferol improved chronic hyperglycemia induced pancreatic β-cells [28]. Another study by Al-Numair et al (2015) reported that kaempferol reduced the blood glucose level and attenuated oxidative stress in streptozotocin (STZ)-induced diabetic rats [16]. A previous study demonstrated that dietary intake of kaempferol significantly reduced hyperglycemia, hyperinsulinemia, and the circulating lipid profile level, and thus improved peripheral insulin sensitivity in obese diabetic mice [19].…”

“…Kaempferol is also present in different medicinal plants like Acacia nilotica (L.) Delile, Aloe vera (L.) Burm.f., and Crocus sativus L. [15]. It has been reported to possess various pharmacological effects such as antioxidant [16], anti-inflammatory [17], anti-apoptotic [18], and anti-diabetic [19] in different experimental models. Kaempferol has been shown to exert an anti-inflammatory effect through inhibition of the MAPK pathway [17].…”

The protective effect of kaempferol on heart via the regulation of Nrf2, NF‐κβ, and PI3K/Akt/GSK‐3β signaling pathways in isoproterenol‐induced heart failure in diabetic rats

“…Kaempferol (KF) is a natural flavonoid found in many plants and plant‐derived foods like Aloe vera , Coccinia grandis , and Moringa oleifera (Calderon‐Montano, Burgos‐Moron, Perez‐Guerrero, & Lopez‐Lazaro, ). KF has been shown to exhibit several pharmacological activities such as antioxidant (Kouhestani, Jafari, & Babaei, ), anti‐inflammatory (Yeon et al, ), neuroprotective (Yu et al, ), antidiabetic (Al‐Numair, Veeramani, Alsaif, & Chandramohan, ), anticancer (Kim, Lee, Kim, Cheon, & Ko, ), and cardioprotective activities (Calderon‐Montano et al, ). Despite of the fact that KF is effective in alleviating various dysfunctions, no studies have demonstrated the effect of KF on HF in diabetic condition.…”

Mechanism of Flavonoids Action in Smooth Muscle Relaxation

“…It is a very well-known to possess antioxidant [107] antiinflammatory [108] , anticancer, [109] neuroprotective [110] and anti-diabetic properties. [111] Influence of kaempferol, a flavonoid compound, on membrane-bound ATPases in streptozotocin-induced diabetic rats. [75] Kaempferol causes endothelium independent relaxation in different animals.…”