Influence of lignin and oxygen on the growth and the lipid formation of the fungus Lentinus tigrinus

Antioxidant effect on the growth and formation of lipids in the fungus Lentinus tigrinus growing on medium with lignosulfonate

“…1 and 2) after the addition of AO to the medium with LSN, which was the only carbon source in the medium for cultivating L. tigrinus. As has been shown previously [7] and was confirmed in this study, the addition of glucose to the medium with LSN resulted in a significant intensification of growth processes; the biomass yield increased (Fig. 1).…”

“…In our previous study we demonstrated that the introduction of 0.5% glucose in fungus growing medium affected the intensity of the growth processes and the composition of lipids: the PA and PI contents changed [7]. However, the PI content increased again if an antioxidant was added in the presence of the min imal amount of glucose (Fig.…”

“…As was mentioned earlier, the growth of fungus L tigrinus in the presence of LSN was accompanied by significant changes in the composition of phospholipids (PL2) [7]. The main differences from the control variant with glucose were a sharp increase in phosphatidic acid (PA), which exceeded the level of phosphatidylethano lamine (PEA) and phosphatidylcholine (PC) by almost 2 times, and a low content of phosphatidylinositol (PI) [7]. The introduction of AO alters the composition of PL-the PA content slightly decreased and the PEA level remained virtually unchanged, but the PC con tent had an almost twofold decrease, while PI signifi cantly increased (Fig.…”

“…Lentinus tigrinus BKM F 160 in the control exper iment was grown in a nutrient medium with glucose (5%) and ammonium nitrate as previously described [7]. In the experiments, glucose was replaced with sodium lignosulfonate (LSN 2%) taken in an equiva lent amount based on carbon.…”

“…An important role in the attack on the polymer lignin belongs to hydroxyl radical ( • ОН), peroxyl radicals (ROO • ), and hydroxy peroxyl radicals ( • ООН). The fact that lignin decomposition by fungi is intensified by the air or oxygen indicates the important role of FRO in these processes [7]. It was suggested that the involvement of FRO in lig nin degradation by fungi can be verified by the inhibi tion of the generation of free radicals by the addition of antioxidants (AO), since two pathways of FRO quenching are known enzymatic via superoxide dis mutase (SOD) and nonenzymatic reactions with AO [4].…”