Influence of long-term CO2 storage on δ13C in vials capped with butyl and butyl/PTFE caps and the relevance for ecological samples

Kuehfuss, Stefanie; Högy, Petra; Fangmeier, Andreas

doi:10.1007/s11104-014-2156-z

Cited by 3 publications

(4 citation statements)

References 34 publications

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“…Our methodology illustrates the need to describe blank corrections and storage artifacts, and how they vary as a function of DIC concentration. Indeed, these features of our analyses help to reconcile previous results in the literature, including those finding large shifts in δ 13 C over short periods of storage (Knohl et al ; Kuehfuss et al ). These deviations likely arose from a small blank that was large relative to the total CO 2 in a low concentration sample.…”

Section: Discussionsupporting

confidence: 90%

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Seawater DIC analysis: The effects of blanks and long‐term storage on measurements of concentration and stable isotope composition

Olack

Colman

Pfister

et al. 2018

Limnology & Ocean Methods

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Dissolved inorganic carbon (DIC) concentration and stable isotope composition measurements (d 13 C) are central to the study of biological and chemical processes in the ocean. We examined a small volume (1 mL) method using continuous-flow isotope ratio mass spectrometry (CF-IRMS) for making both measurements in a single sample run. Prior exploration of this approach has yielded variable results. Effects of concentration, isotopic composition, storage time, and the gas used to flush the sample tubes were tested using standard solutions. Multiple isotopic composition standards permitted calculation of an apparent blank that develops due to slow rates of gas exchange through the septum. A blank correction is applied to the d 13 C measurements, giving precision and accuracy better than 0.1& after 6 months of storage and 0.2& after 18 months of storage. CF-IRMS concentration measurements on field samples collected near Tatoosh Island, off the Pacific coast of Washington, U.S.A., were compared to independent measurements (Dickson, CDQC Laboratory, UCSD) on the same water, the latter approach involving collection of 500 mL of seawater fixed with mercuric chloride. Concentration measurements by CF-IRMS on seawater samples (precision 1-2%) are correctable to the Dickson results, though scatter in this relation exceeds analytical precision. The DIC and d 13 C measurements on coastal seawater, in conjunction with salinity measurements, indicated strong effects of biological activity on carbon dynamics relative to possible effects of mixing water masses. In sum, this small volume methodology has application to field situations and laboratory experiments where larger volumes are logistically challenging and storage space is limited.

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Section: Discussionsupporting

confidence: 90%

“…The above two studies bracket the expected concentration of approximately 0.4% CO 2 derived from marine DIC samples . Tests on 100% CO 2 samples showed large δ 13 C shifts almost immediately, growing to a 1.7‰ shift, which then may have plateaued after 100 d (Kuehfuss et al ).…”

mentioning

confidence: 99%

Seawater DIC analysis: The effects of blanks and long‐term storage on measurements of concentration and stable isotope composition

Olack

Colman

Pfister

et al. 2018

Limnology & Ocean Methods

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“…[1][2][3] Limited work has been conducted to date on δ 13 C fractionation effects in free-gas mixtures, with much of the work focused on CO 2 . [4][5][6][7][8][9] Dissolved-gas containers were discussed by Weimer and Lee [10] although no study to our knowledge has yet examined isotopic effects.…”

mentioning

confidence: 99%

“…Previous studies have examined the efficacy of free‐gas containers for sample storage prior to gas concentration analyses including effects such as piercing of septa and air transportation . Limited work has been conducted to date on δ 13 C fractionation effects in free‐gas mixtures, with much of the work focused on CO 2 . Dissolved‐gas containers were discussed by Weimer and Lee although no study to our knowledge has yet examined isotopic effects.…”

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confidence: 99%

Suitability of selected free‐gas and dissolved‐gas sampling containers for carbon isotopic analysis

Eby

Gibson

2015

Rapid Comm Mass Spectrometry

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Evaluation of different samplers and storage temperature effect on the methane carbon stable isotope analysis

Santos

Laroque

Baum

et al. 2018

Environ Monit Assess

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The present work evaluates the efficiency of some low-cost sampler container for a reliable carbon stable isotope analysis of methane. The procedure efficiency was evaluated for five containers, through 91 days, under two storage temperatures (4 °C and 25 °C) and the results are compared against a reference sampler by using univariate and multivariate statistical methods. Based on the univariate (ANOVA and comparison statistical methods) and multivariate (PCA and HCA) statistical methods, it was identified that (i) the isotopic value changes with time and, in this way, must be taken in account when choosing the appropriate sampler and (ii) the lower temperature reduces the isotopic fractionation process and is preferable for the gas sample storage. Among the storage systems, two options were found to be statistically equivalent to the reference container (IsoJar) for a time horizon of 91 days. We found that the exetainer (4 °C and 25 °C) storage systems are statistically equivalent to the reference container IsoJar and, in this way, it could be an alternative for the methane isotopic studies.

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Influence of long-term CO2 storage on δ13C in vials capped with butyl and butyl/PTFE caps and the relevance for ecological samples

Cited by 3 publications

References 34 publications

Seawater DIC analysis: The effects of blanks and long‐term storage on measurements of concentration and stable isotope composition

Seawater DIC analysis: The effects of blanks and long‐term storage on measurements of concentration and stable isotope composition

Suitability of selected free‐gas and dissolved‐gas sampling containers for carbon isotopic analysis

Evaluation of different samplers and storage temperature effect on the methane carbon stable isotope analysis

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