Influence of long term exposure to space flight on tomato seeds

Nechitailo, Galina S.; Jinying, Lu; Han, Xue; Pan, Yi; Chong-qin, Tang; Liu, Min

doi:10.1016/j.asr.2005.06.043

Cited by 33 publications

(22 citation statements)

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“…As a result, new genetic variations are generated, from which desired mutants can be selected (Novak and Brunner, 1992). Although the genetic mechanism of the induced mutations is unknown, space flight is considered an effective approach for acquiring new favorable alleles and improving elite germplasm for breeding and is used to induce mutations in seeds of Arabidopsis (Kranz, 1986), wheat (Tripathy et al, 1996), rice (Yu et al, 2007), and other plants (Nechitailo et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Identification of a major quantitative trait locus for ear size induced by space flight in sweet corn

Yu¹,

Li²,

Yang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The development of molecular markers has contributed to progress in identifying the gene(s) responsible for favorable variations in maize studies. In this study, quantitative trait locus (QTL) mapping was conducted using simple sequence repeat markers in an F 2 sweet corn population from a cross between parental line 1132 and space flight-induced mutant line 751 to identify the loci contributing to an increase in some yield traits. A primary mutated genomic region was located on chromosome 9. In total, 26 QTL were detected for eight yield-related traits and assembled into three clusters on chromosome 9.

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Section: Introductionmentioning

confidence: 99%

Identification of a major quantitative trait locus for ear size induced by space flight in sweet corn

Yu¹,

Li²,

Yang³

et al. 2014

Genet. Mol. Res.

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“…Many space flight experiments have been conducted on tomatoes, potatoes and fungi with results showing morphological variation and DNA mutation [12,13] . Gao investigated the influence of space flight on the medicinal plants Platycodon grandiflorus, and Agastache rugosa utilizing an electron microscope.…”

Section: Discussionmentioning

confidence: 99%

Effects of space flight on DNA mutation and secondary metabolites of licorice (Glycyrrhiza uralensis Fisch.)

Gao

Yan

et al. 2009

SCI CHINA SER C

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Licorice (Glycyrrhiza uralensis Fisch.) seeds were flown on a recoverable satellite for 18 days(the average radiation dose in the flight recovery module was 0.102 mGy/d, the distance from flight apogee to earth was 350 km, gravity 10(-6)). After returning to earth, the seeds were germinated and grown to maturity. The parallel ground-based seeds were also planted under the same conditions. The leaves of licorice were used for inter-simple sequence repeat (ISSR) analysis and the two main secondary metabolites in one-year-old roots were analyzed by high performance liquid chromatography (HPLC). Among 22 random primers used in this experiment, 6 primers generated different DNA band types. Analysis of HPLC showed that the content of glycyrrhizic acid (GA) and liquiritin (LQ) in the roots from seeds flown in space was respectively 2.19, 1.18 times higher than that of the control group. The results demonstrated that the extraterrestrial environment induced mutagenic effects on licorice and affected its secondary metabolites. These changes indicated that extraterrestrial orbit is possible means of breeding of licorice so as to preserve this endangered medicinal plant.

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“…Their results also showed that the genetic diversity of the spaceflight specimens was higher than that of the ordinary specimens. Nechitailo et al (2005) conducted an amplification analysis on the genomic DNA of tomato seedlings grown from seeds carried in the Russian MIR space station using RAPD technology. The results showed that, compared with the controls, plants from seeds carried in the space station generated different band patterns of amplified fragments.…”

Section: Discussionmentioning

confidence: 99%

“…However, the molecular effects of space environments on plants are largely unknown. Molecular-marker techniques have been widely used for detecting genetic mutations after space flight, including inter-simple sequence repeat markers (Gao et al, 2009;Wu et al 2011), simple sequence repeat (SSR) markers (Zhou et al, 2007;Xiao et al, 2009;He et al, 2010;Lu et al, 2010), amplified fragment length polymorphism markers (Yi et al, 2002;Li et al, 2007;Lu et al, 2010;Sun et al, 2010), random amplification of polymorphic DNA (RAPD) markers (Liu et al, 1999;Gao et al, 2000;Yi et al, 2002;Nechitailo et al, 2005;Xie et al, 2010), and sequence-related amplified polymorphism (SRAP) markers Wang et al, 2009). Among these molecular markers, SSRs are often employed because of their reproducibility, codominant inheritance, relative abundance, multiallelic nature, good genome coverage, and high frequency of polymorphism detection (Powell et al, 1996;McCouch et al, 2002;Lu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity and variation of Robinia pseudoacacia seeds induced by short-term spaceflight based on two molecular marker systems and morphological traits

Yuan¹,

Li²,

Sun³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. The black locust (Robinia pseudoacacia) is a forest legume that is highly valued as a honey plant and for its wood. We explored the effect of short-term spaceflight on development of R. pseudoacacia seedlings derived from seeds that endured a 15-day flight; the genetic diversity and variation of plants sampled from spacemutagenized seeds were compared to plants from parallel groundbased control seeds using molecular markers and morphological traits. In the morphology analysis, the space-mutagenized group had 4269 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 4268-4277 (2012) Genetic diversity and variation of Robinia pseudoacacia apparent variation compared with the control group in morphological traits, including plant height, basal diameter, number of branches, branch stipular thorn length, branch stipular thorn middle width, leaflet vertex angle, and tippy leaf vertex angle. Simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) molecular marker analyses showed a slightly higher levels of genetic diversity in the space-mutagenized group compared to the control group. In the SRAP analysis, the space-mutagenized group had 115 polymorphic bands vs 98 in the controls; 91.27% polymorphic loci vs 77.78% in the controls; 1.9127 ± 0.2834 alleles vs 1.7778 ± 0.4174 in the controls; Nei's genetic diversity (h) was 0.2930 ± 0.1631 vs 0.2688 ± 0.1862 in the controls, and the Shannon's information index (I) was 0.4452 ± 0.2177 vs 0.4031 ± 0.2596 in the controls. The number of alleles was significantly higher in the space-mutagenized group. In the SSR analysis, the space-mutagenized group also had more polymorphic bands (51 vs 46), a greater percentage of polymorphic loci (89.47% vs 80.70%); h was also higher (0.2534 ± 0.1533 vs 0.2240 ± 0.1743), as was I (0.3980 ± 0.2069 vs 0.3501 ± 0.2412). These results demonstrated that the range of genetic variation in the populations of R. pseudoacacia increased after spaceflight. It also suggested that the SSR and SRAP markers are effective markers for studying mutations and genetic diversity in R. pseudoacacia. The data provide valuable molecular evidence for the effects of the space environment on R. pseudoacacia and may contribute to future spacebreeding programs involving forest trees.

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Influence of long term exposure to space flight on tomato seeds

Cited by 33 publications

References 1 publication

Identification of a major quantitative trait locus for ear size induced by space flight in sweet corn

Identification of a major quantitative trait locus for ear size induced by space flight in sweet corn

Effects of space flight on DNA mutation and secondary metabolites of licorice (Glycyrrhiza uralensis Fisch.)

Assessment of genetic diversity and variation of Robinia pseudoacacia seeds induced by short-term spaceflight based on two molecular marker systems and morphological traits

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