Influence of <em>FTO</em> rs9939609 polymorphism on appetite, ghrelin, leptin, IL6, TNF&alpha; levels, and food intake of women with morbid obesity

Abstract

BackgroundThe fat mass and obesity-related (FTO) gene has a strong relationship with obesity, extreme obesity and inflammatory state, and may also be associated with food intake regulation.ObjectiveThe aim of the present study was to evaluate the influence of the rs9939609 single-nucleotide polymorphism of the FTO gene on appetite, ghrelin, leptin, interleukin 6 (IL6), tumor necrosis factor α (TNFα) levels and food intake of morbidly obese women.Materials and methodsThe study comprised 70 women, aged between 20 and 48 years, from Rio de Janeiro, Brazil. The participants were selected according to the body mass index between 40 and 60 kg/m2. Anthropometric and biochemical data were measured during fasting. Hormones and inflammatory data were measured before and after the participants ate an isocaloric meal. Dietary records were calculated and analyzed using a nutritional assessment program. Visual analog scales were used for behaviors of the sensations of appetite and food preferences. The FTO rs9939609 variant was genotyped using real-time polymerase chain reaction.ResultsParticipants with the AA genotype had lower values of ghrelin and IL6 and higher values of leptin than those with TT and TA in the postprandial period. Comparing the plasma concentrations of ghrelin, insulin, IL6 and TNFα intragenotypes, it was observed that those with TT had decreased leptin and increased IL6 at the postprandial period. Subjects with TA showed increased postprandial IL6, and those with AA had decreased postprandial ghrelin. There was no difference in TNFα intra- and intergenotypes. The postprandial sensations of hunger were lower in AA than those with TT. There were differences between genotypes regarding ingested grams of protein by weight, cholesterol, B3, B5, B6 and B12 vitamins, and selenium potassium and sodium minerals.ConclusionThese findings suggest that genetics may exert an influence on physiologic factors and might alter eating behavior.

Exploration of associations between the FTO rs9939609 genotype, fasting and postprandial appetite-related hormones and perceived appetite in healthy men and women

“…whereas we adopted a multivariate-adjusted approach to our data analysis. Interestingly, recent studies have reported lower postprandial total ghrelin concentrations in AA compared to AT and TT individuals (Magno et al 2018;Melhorn et al 2018), and postprandial hunger ratings were either similar between genotype groups (Melhorn et al 2018) or were lower in AA individuals (Magno et al 2018). These findings were observed despite the AA individuals exhibiting higher energy intake during an ad libitum buffet (Melhorn et al 2018).…”

FTO Variant rs1421085 Associates With Increased Body Weight, Soft Lean Mass, and Total Body Water Through Interaction With Ghrelin and Apolipoproteins in Arab Population

“…However, rs9939609 was associated with increased TNFa in Mexican women with gestational diabetes (Saucedo et al, 2017). The impact of rs9939609 on the fasting and postprandial level of IL-6 and TNFa in Brazilian women revealed that, in the fasting stage, levels of IL-6 and TNFa were similar for the A and T risk alleles but in the postprandial period, while TNFa was not altered in individuals with AA genotype compared to TT and TA genotypes, the serum levels of IL-6 and ghrelin were low and those of leptin were high (Magno et al, 2018). Ethnicity is likely to have an effect on the variation in association of FTO with inflammatory markers and investigating these variants may offer a better understanding of their role in the obesity epidemic affecting the world and particularly countries such as Kuwait and others in the Gulf region.…”

In depth analysis of the association of FTO SNP (rs9939609) with the expression of classical phenotype of PCOS: a Sri Lankan study

“…Rutters et al found FTO A allele to be associated with higher BMI and leptin levels at the age of 12, with the association being stronger at the age of 17 [44]. Magno et al showed that those with the AA genotype had higher values of leptin than those with TT and AT [45]. Yet others have reported the expression of Kiss1 gene (gene that produces kisspeptin) may be induced by leptin [46,47].…”