Influence of macroporous gold support and its functionalization on lactate oxidase-based biosensors response

Gamero, M.; Sosna, Maciej; Pariente, F.; Lorenzo, Encarnación; Bartlett, P. N.; Alonso, C.

doi:10.1016/j.talanta.2012.03.051

Cited by 33 publications

(18 citation statements)

References 25 publications

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“…A possible solution to this problem is to utilize high current density redox mediators to facilitate electron transfer from the flavin adenine dinucleotide (FAD) active site of LOx to the electrode surface at a lower potential than that required for H2O2 oxidation. (Casero et al 2014;Gamero et al 2012;Qin et al 2012) Additionally, such materials can be coupled with a biocathode to provide the electrochemical driving force required for lactate detection; such a device can then function as a self-powered lactate sensor to eliminate the need for an external power source.…”

Section: Introductionmentioning

confidence: 99%

A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI

Hickey

Reid

Milton

et al. 2016

Biosensors and Bioelectronics

142

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Lactate is an important biomarker due to its excessive production by the body during anerobic metabolism. Existing methods for electrochemical lactate detection require the use of an external power source to supply a positive potential to the working electrode of a given device. Herein we describe a self-powered amperometric lactate biosensor that utilizes a dimethylferrocene-modified linear poly(ethylenimine) (FcMe2-LPEI) hydrogel to simultaneously immobilize and mediate electron transfer from lactate oxidase (LOx) at the anode and a previously described enzymatic cathode. Operating as a half-cell, the FcMe2-LPEI electrode material generates a jmax of 1.51 ± 0.13 mAcm(-2) with a KM of 1.6 ± 0.1 mM and a sensitivity of 400 ± 20 μAcm(-2)mM(-1) while operating with an applied potential of 0.3 V vs. SCE. When coupled with an enzymatic biocathode, the self-powered biosensor has a detection range between 0mM and 5mM lactate with a sensitivity of 45 ± 6 μAcm(-2)mM(-1). Additionally, the FcMe2-LPEI/LOx-based self-powered sensor is capable of generating a power density of 122 ± 5 μWcm(-2) with a current density of 657 ± 17 μAcm(-2) and an open circuit potential of 0.57 ± 0.01 V, which is sufficient to act as a supplemental power source for additional small electronic devices.

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Section: Introductionmentioning

confidence: 99%

A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI

Hickey

Reid

Milton

et al. 2016

Biosensors and Bioelectronics

142

View full text Add to dashboard Cite

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“…Nevertheless, it is necessary to say that 7.5 is the highest number of layers assayed because the amplitude of the wave in the chronoamperometric experiment employed to obtain the electrodeposited gold, decreases as the number of sphere layers increases 45. Values above 7.5 give rise to currents too low to allow the thickness control.…”

Section: Resultsmentioning

confidence: 99%

“…Then a potential of −1.05 V vs. Hg/HgSO 4 was applied until the required charge had been passed. After the electrodeposition the polystyrene template was removed by dissolving in dimethylformamide in the presence of ultrasound for at least 1 h, leading to the desired macroporous structure with a thickness controllable by the charge used during the electrodeposition step 45.…”

Section: Methodsmentioning

confidence: 99%

Electrocatalytic Activity of 3‐Dimensional Ordered Macroporous Gold Electrode‐Based Lactate Biosensors Platforms as a Function of Pore Layer Number

et al. 2012

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We report the electrocatalytic response of a lactate biosensor, based on a 3‐dimensional ordered macroporous (3DOM) gold electrode, as a function of the pore layer number. The biosensor was developed by covalent immobilization of lactate oxidase (LOx) to a self‐assembled monolayer of dithiobis‐N‐succinimidyl propionate (DTSP). The binding of DTSP to the gold surface was studied by X‐ray photoelectron spectroscopy (XPS), indicating the existence of SAu bonds. The DTSP coverage and the different steps followed in the construction of the biosensor were examined by electrochemical impedance spectroscopy (EIS). The catalytic response to a 14 mM lactate concentration, increased linearly with the pore layer number of the 3DOM gold electrode, reaching a value 3 times higher, than for polycrystalline gold electrodes, for 7.5 layers.

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“…Macroporous materials, such as inverse opal materials (IOM), have received increasing interest in the last decades due to their regular porous structure, large surface area, and porosity, [1][2][3] which have great potential application in catalysis, [4][5][6][7][8][9] fuel cells, [10][11][12] photonic materials, [13][14][15][16] and framework. For instance, Stein and co-workers prepared the zeolite silicalite by multistep hydrothermal reactions in a carbon inverse opal template.…”

Section: Introductionmentioning

confidence: 99%

Macroporous Polytetrafluoroethylene Film with a Reusable Matrix and Its Application as the Microreactors

Wang

Zhao

Wang

et al. 2016

Macro Materials & Eng

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sensors. [ 17,18 ] IOM can be readily fabricated using colloidal crystal ball or fi lm as the template, followed by fi lling the solution of the framework material or its precursors into the voids of the colloidal crystal array, and then removing the original colloidal crystal template by wet chemical etching or calcination. A great variety of inverse opal materials have been prepared using this method [ 14,[19][20][21][22][23] after the fi rst report was published on Nature in 1997. [ 24 ] At the same time, the application of IOM has been extensively studied. Recently, the application of IOM in the preparation of inorganic microparticles has attracted much more attention, [25][26][27][28][29] because the monodispersed voids in IOM can be used as the ideal uniform microreactors or micromolds to obtain various particles with narrow size distribution, including metals, [ 27 ] oxides, [ 30 ] carbonates, [ 31 ] and polymers. [ 32 ] The target microparticles can be easily collected by dissolving or thermally decomposing the IOM This work reports a simple and effi cient preparation method of an inverse-opal-like macroporous polytetrafl uoroethylene (IO-PTFE) fi lm with a reusable matrix. At fi rst, the pH-responsive poly(acrylic acid) (PAA) is grafted on the PTFE latex particles induced by γ-ray radiation. The stability of PAA grafted PTFE latex particles (PTFE-g -PAA) in water decreases in acid aqueous solution. As a result, PTFE-g -PAA particles can coprecipitate with monodispersed polystyrene (PS) microspheres from the acidic aqueous dispersion to form a composite particulate fi lm. After the composite particulate fi lm is etched with toluene to remove PS microspheres, a selfstand inverse-opal-like macroporous PTFE fi lm can be successfully obtained. The macroporous PTFE fi lm can be applied as the microreactor to synthesize uniform TiO 2 particles. The separation of TiO 2 microparticles and the recycle of PTFE-g -PAA particles can be simultaneously achieved simply in water by means of ultrasonifi cation. The prepared TiO 2 microparticles also show good photocatalytic performance. This work opens a new thought to prepare special macroporous polymeric materials, and expands the microsynthesis of inorganic or organic microparticles by taking advantage of polymeric macroporous materials.

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Influence of macroporous gold support and its functionalization on lactate oxidase-based biosensors response

Cited by 33 publications

References 25 publications

A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI

A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI

Electrocatalytic Activity of 3‐Dimensional Ordered Macroporous Gold Electrode‐Based Lactate Biosensors Platforms as a Function of Pore Layer Number

Macroporous Polytetrafluoroethylene Film with a Reusable Matrix and Its Application as the Microreactors

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