Influence of magnesium sulfate on HCO 3 /Cl transmembrane exchange rate in human erythrocytes

Chernyshova, Ekaterina S.; Zaikina, Yulia S.; Tsvetovskaya, Galina A.; Strokotov, Dmitry I.; Yurkin, Maxim A.; Serebrennikova, Elena S.; Volkov, Léonid; Maltsev, Valeri P.; Chernyshev, Andrei V.

doi:10.1016/j.jtbi.2015.12.023

Cited by 14 publications

(18 citation statements)

References 53 publications

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“…Band 3 protein is the most abundant integral membrane protein of erythrocytes [12,13], responsible for Cl -/HCO 3 exchange, which correlates with ion balance across membrane, osmotic properties, cell shape maintaining, gas exchange and, in turn, oxygenation of the entire organism [14]. These functions are mediated by both membrane and cytoplasmic domains via phosphorylation pathways, as recently shown [15,16].…”

Section: Introductionmentioning

confidence: 95%

“…uptake [12], significantly accounts for Cl − /HCO 3 − exchange and can be monitored by a turbidimetric method [12][13][14][15][16][17][18][19] based on measurement of the rate constant for SO 4 = uptake. This method, enabling determination of anion exchange velocity, has been recently confirmed as a good tool to verify the impact of oxidative stress on human erythrocytes exposed to xenobiotics, natural toxins or hydrogen peroxide [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Band 3 Protein Function and Oxidative Stress in Erythrocytes from Systemic Sclerosis Patients with Interstitial Lung Disease

Morabito¹

2017

EJCBS

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A link between reactive oxygen species (ROS) and Systemic Sclerosis (SSc) development has been already described, along with an oxidative stress-induced reduction of anion exchange capability through Band 3 protein. On this basis, blood from SSc patients has been collected to measure the rate constant for SO 4 = uptake, which is an index for anion exchange capability through Band 3 protein. This parameter was significantly reduced in SSc patients, with or without Interstitial Lung Disease, a complication of SSc. As a reduced Band 3 protein function was observed in SSc patients with either low or high levels of malonildialdehyde (MDA), the end product of lipoperoxidation, anion exchange through erythrocytes membrane may be not univocally related to oxidative stress and, what is more, can be considered as a sensitive tool to monitor SSc development.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Band 3 Protein Function and Oxidative Stress in Erythrocytes from Systemic Sclerosis Patients with Interstitial Lung Disease

Morabito¹

2017

EJCBS

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“…Magnesium, the second most abundant intracellular ion after K + , with concentrations ranging between 5 and 30 mM, modulates cell volume regulation, enzymes activity and erythrocytes membrane physical properties [44]. Previous studies on the effect of Mg 2+ deficiency on membrane function and red blood cell metabolism have been performed in animal models [43,45] and in oxidative stress-related diseases, such as preeclampsia, with hypoxia due to preterm labor [46]. It has already been reported that, in hamsters, magnesium deficiency increases red blood cells' susceptibility to injury due to free radicals and, in rats, provokes a significant reduction in red blood cells GSH, restored by administration of d-propanolol or vitamin E [47,48].…”

Section: Magnesiummentioning

confidence: 99%

Natural Antioxidants Beneficial Effects on Anion Exchange through Band 3 Protein in Human Erythrocytes

Morabito¹,

Marino²

2019

Antioxidants

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Band 3 protein (B3p) exchanging Cl− and HCO3− through erythrocyte membranes is responsible for acid balance, ion distribution and gas exchange, thus accounting for homeostasis of both erythrocytes and entire organisms. Moreover, since B3p cross links with the cytoskeleton and the proteins underlying the erythrocyte membrane, its function also impacts cell shape and deformability, essential to adaptation of erythrocyte size to capillaries for pulmonary circulation. As growing attention has been directed toward this protein in recent years, the present review was conceived to report the most recent knowledge regarding B3p, with specific regard to its anion exchange capability under in vitro oxidative conditions. Most importantly, the role of natural antioxidants, i.e., curcumin, melatonin and Mg2+, in preventing detrimental oxidant effects on B3p is considered.

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“…However only a few publications, so far, describe that divalent metal ions like Mg 2+ and Zn 2+ can modulate the transport activity 18 – 20 . The electrogenic activity of NBCe1 was inhibited by Mg 2+ ( K i of 0.01 mM) in whole-cell patch-clamp studies 18 , and the transport activity by AE1 was predicted to be stimulated by intracellular levels of Mg 2+ 19 . AE2 show partial inhibition by extracellular Zn 2+ when expressed in Xenopus oocytes 20 while equivalent concentration of extracellular Zn 2+ had no effect on AE1 activity 20 .…”

Section: Introductionmentioning

confidence: 99%

The crystal structure of the regulatory domain of the human sodium-driven chloride/bicarbonate exchanger

Alvadia

Sommer

Bjerregaard-Andersen

et al. 2017

Sci Rep

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The sodium-driven chloride/bicarbonate exchanger (NDCBE) is essential for maintaining homeostatic pH in neurons. The crystal structure at 2.8 Å resolution of the regulatory N-terminal domain of human NDCBE represents the first crystal structure of an electroneutral sodium-bicarbonate cotransporter. The crystal structure forms an equivalent dimeric interface as observed for the cytoplasmic domain of Band 3, and thus establishes that the consensus motif VTVLP is the key minimal dimerization motif. The VTVLP motif is highly conserved and likely to be the physiologically relevant interface for all other members of the SLC4 family. A novel conserved Zn2+-binding motif present in the N-terminal domain of NDCBE is identified and characterized in vitro. Cellular studies confirm the Zn2+ dependent transport of two electroneutral bicarbonate transporters, NCBE and NBCn1. The Zn2+ site is mapped to a cluster of histidines close to the conserved ETARWLKFEE motif and likely plays a role in the regulation of this important motif. The combined structural and bioinformatics analysis provides a model that predicts with additional confidence the physiologically relevant interface between the cytoplasmic domain and the transmembrane domain.

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Influence of magnesium sulfate on HCO 3 /Cl transmembrane exchange rate in human erythrocytes

Cited by 14 publications

References 53 publications

Band 3 Protein Function and Oxidative Stress in Erythrocytes from Systemic Sclerosis Patients with Interstitial Lung Disease

Band 3 Protein Function and Oxidative Stress in Erythrocytes from Systemic Sclerosis Patients with Interstitial Lung Disease

Natural Antioxidants Beneficial Effects on Anion Exchange through Band 3 Protein in Human Erythrocytes

The crystal structure of the regulatory domain of the human sodium-driven chloride/bicarbonate exchanger

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