Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

Kremplová, Monika; Fialová, Dana; Nejdl, Lukáš; Hynek, David; Trnková, Libuše; Hubálek, Jaromír; Kizek, René; Adam, Vojtěch

doi:10.3390/ma7031455

Cited by 5 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MPs are a suitable tool for DNA isolation and manipulation allowing for the purification of DNA from various matrices [ 34 , 35 , 36 , 37 ]. Various modifications of the magnetic microparticles could provide the specific binding to analytes [ 38 , 39 , 40 ].…”

Section: Resultsmentioning

confidence: 99%

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

Skaličková

Nejdl

Kudr

et al. 2016

Sensors

Self Cite

View full text Add to dashboard Cite

Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL−1, respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.

show abstract

Section: Resultsmentioning

confidence: 99%

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

Skaličková

Nejdl

Kudr

et al. 2016

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…Adenosine tri phosphate is the nitrogenous base adenine bonded to a five carbon sugar. Adenosine tri phosphate is used in cellular metabolism as one of the basic methods of transferring chemical energy between, chemical reactions [10]. Adenine is one of the two purine nucleo bases used in forming nucleotides of the nucleic acids DNA and RNA.…”

Section: Adenine Metabolismmentioning

confidence: 99%

Biological Study of Transition Metal Complexes with Adenine Ligand

Hasan

Yahiya

Hassan

et al. 2019

The 23rd International Electronic Conference on Synthetic Organic Chemistry

View full text Add to dashboard Cite

Adenine complexes were prepared with some of the first series transition metals in a stoichiometric ratio of 1: 2 (M n+ : L), where M n+ = Mn 2+ , Fe 3+ , Co 2+ , Ni 2+ , Cu 2+ , Zn 2+ , and Cd 2+ ions. The Complexes were characterized by the physicochemical and spectroscopic techniques as electric conductivity, metal contents, IR, UV-Visible, and molar conductance techniques. The stoichiometric ratios of the synthesized complexes were confirmed by using molar ratio method. The dissociation constant of adenine ligand was determined spectrophotometrically. Solvent effect on the electronic spectra of the adenine ligand was examined using solvents with different polarities. The biological activity of adenine ligand and its metal complexes were tested in vitro against some selected species of fungi and bacteria. The results showed a satisfactory spectrum against the tested organisms.

show abstract

Functionalized magnetic particles coupled with LC-MS strategy facilitated discovery of trace thioalkaloids with potent immunosuppressive activity

Lin,

Li,

Liu

et al. 2024

Bioorganic Chemistry

View full text Add to dashboard Cite

Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

Cited by 5 publications

References 54 publications

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

Biological Study of Transition Metal Complexes with Adenine Ligand

Functionalized magnetic particles coupled with LC-MS strategy facilitated discovery of trace thioalkaloids with potent immunosuppressive activity

Contact Info

Product

Resources

About