Influence of Material and Surface Roughness of Resin Composite Cements on Fibroblast Behavior

Rohr, Nadja; Bertschinger, N; Fischer, Jens; Filippi, Andreas; Zitzmann, NU

doi:10.2341/19-113-l

Cited by 15 publications

(21 citation statements)

References 36 publications

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“…Surface roughness has been reported to influence cell viability and morphology. 17,21,22 However, in the present study, based on the low viability values for unpolished printed specimens, it can be assumed that leaching components had a more pronounced effect than surface roughness. The biocompatibility of resin materials may be reduced by unreacted monomers that can react with adjacent tissue.…”

Section: Discussionmentioning

confidence: 56%

“…10,12,[14][15][16] Human gingival fibroblast (HGF-1) cells, found within the junctional epithelium and connective tissue, have been commonly used to assess the biocompatibility of dental materials. [17][18][19] The behavior of HGF-1 cells regarding cell adhesion and growth on different surface topographies has been reported, and analysis of the cell morphology allows an estimation of the cell reaction to a material. 17,[20][21][22] HGF-1 cells also show high sensitivity in cytotoxicity tests, 3 typically with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) 3,23 24 The purpose of the present in vitro study was to evaluate the cell behavior of HGF-1 on different occlusal device materials that were conventionally processed, milled, or printed.…”

mentioning

confidence: 99%

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Fibroblast behavior on conventionally processed, milled, and printed occlusal device materials with different surface treatments

Bieger¹,

Thieringer

Fischer³

et al. 2023

The Journal of Prosthetic Dentistry

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Statement of problem. Occlusal devices can be either conventionally processed, milled, or printed. However, little is known about the biocompatibility of 3D printing resin materials.Purpose. The purpose of this in vitro study was to compare the viability and morphology of human gingival fibroblast cells (HFG-1) after cultivation on conventionally processed, milled, and printed occlusal device materials with different surface treatments. Material and methods. Disks of a conventionally processed (PalaXpress Clear [pP]), milled (Yamahachi PMMA Clear [sY]), and 2 different printed materials (Dental LT Clear Resin [aD];Freeprint splint [aF]) were prepared. The surfaces of the specimens were finished by using 2 different treatments (unpolished and polished with P1200-grit silicon carbide paper). HGF-1 cells were cultivated on the specimens for 24 hours, and a viability assay was performed by using polystyrene disks as a control (n=9 disks per group). Cell morphology and the topography of the specimens were examined with scanning electron microscopy (n=3 disks per group). Two-way analysis of variance was applied to determine the effect of material and surface treatment followed by the post hoc Fisher least significant difference test (a=.05).Results. Overall, material (P<.001) and surface treatment (P<.001) significantly influenced the viability of HGF-1 cells. The viability of cells on all specimens displayed mean values between 0.85 and 1.01 compared with the control except for unpolished aD (0.00 ±0.07) and aF (0.02 ±0.05) that had only a few cells with a round shape.Conclusions. The behavior of HGF-1 cells on conventionally processed and milled specimens was similar and not dependent on the surface treatment. Unpolished printed specimens had a cytotoxic effect. However, after polishing, cell behavior was similar to that of the conventionally processed and milled specimens.

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Section: Discussionmentioning

confidence: 56%

mentioning

confidence: 99%

Fibroblast behavior on conventionally processed, milled, and printed occlusal device materials with different surface treatments

Bieger¹,

Thieringer

Fischer³

et al. 2023

The Journal of Prosthetic Dentistry

View full text Add to dashboard Cite

show abstract

“…Hence, the factor micro-gap was eliminated. However, excess cement with its rough surface reduces fibroblast viability 41 and may consequently affect soft tissue adaption or bone formation. 42 F I G U R E 4 Mean DIB and standard deviation indicating the location of the first bone implant contact (fBIC) on the implant topography.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, the factor micro‐gap was eliminated. However, excess cement with its rough surface reduces fibroblast viability 41 and may consequently affect soft tissue adaption or bone formation 42 …”

Section: Discussionmentioning

confidence: 99%

Influence of zirconia implant surface topography on first bone implant contact within a prospective cohort study

Rohr

Balmer

Jung

et al. 2021

Clin Implant Dent Rel Res

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Background Although 5‐year clinical data exist for different zirconia implants, no analysis has yet been performed focusing on how the surface topography of the implant affects clinical parameters. Purpose To analyze the influence of zirconia implant topography on first bone implant contact (fBIC). Materials and Methods In a prospective two‐center cohort investigation 63 zirconia implants were evaluated at implant placement, prosthetic delivery, 1, 3, and 5 years. The distance (DIB) between implant shoulder and fBIC was measured at each time point in periapical radiographs at mesial and distal sites. Two‐way ANOVA/Bonferroni was used to analyze the effects of time and center (α < 0.05). Results Between the centers, the mean DIB varied significantly at implant placement (Freiburg [FR]: 1.4 ± 0.6 mm; Zurich [ZH]: 0.8 ± 0.5 mm). Thereafter, no statistically significant difference in DIB was observed, neither between centers nor between time points (prosthetic delivery: FR: 1.9 ± 0.6 mm, ZH: 1.7 ± 0.8 mm; 1 year: FR: 1.8 ± 0.6 mm, ZH: 1.6 ± 0.8 mm; 3 years: FR: 1.9 ± 0.8 mm, ZH: 1.7 ± 0.8 mm; 5 years: FR: 1.9 ± 0.8 mm, ZH 1.8 ± 0.6 mm). The overall mean DIB at prosthetic delivery to 5 years of both centers (1.8 ± 0.7 mm) is located within the transition zone between the smooth neck and the moderately rough intraosseous part (1.6–2.0 mm from the implant shoulder). However, individual DIB values are ranging from 0.1 to 4.2 mm overlapping the transition zone. Conclusions The standard deviation of the DIB indicates that the fBIC establishes on moderately rough and smooth surfaces. Consequently, soft tissue adapts to both topographies as well.

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“…When using conventional cements such as zinc phosphate or glass ionomer cements, retentive preparations and thin cement margins are required to avoid hydrolysis of exposed cement material. Resin composite cements facilitate adhesion to tooth or implant abutments so that retention is not required and noninvasive preparation designs are feasible [ 6 , 9 , 10 ]. Resin composite cements are characterized by higher strength, lower cement wear, and improved esthetics compared to conventional cements [ 11 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior

et al. 2022

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Background Human gingival fibroblast (HGF-1) cells in the connective tissue provide an effective barrier between the alveolar bone and the oral environment. Cement margins of restorations with intrasulcular preparation or cemented implant restorations are in contact with HGF cells. However, it is unknown to what extend the cement surface finish affects the behavior of HGF cells. The purpose of this study was to compare the behavior of HGF-1 cells in contact with two different resin composite cements with three different surface treatments after light-curing and autopolymerization, respectively. Methods Disks of one adhesive (Multilink Automix, Ivoclar Vivadent [MLA]) and one self-adhesive (RelyX Unicem 2 Automix, 3 M [RUN]) resin composite cement were either light-cured or autopolymerized. Specimen surfaces were prepared with the oxygen inhibition layer intact, polished with P2500-grit silicon carbide paper or treated with a scaler. Cells were cultivated on the specimens for 24 h. Viability assay was performed, and cell morphology was examined with scanning electron microscopy. Additionally, roughness parameters of the specimen were analyzed with a 3D laser scanning microscope. Three-way ANOVA was applied to determine the effect of cement material, curing mode and surface treatment (a = 0.05). Results Overall, cement material (p = 0.031), curing mode (p = 0.001), and surface treatment (p < 0.001) significantly affected relative cell viability of HGF. The autopolymerized specimen with the oxygen inhibition layer left intact displayed the lowest relative cell viability (MLA 25.7%, RUN 46.6%). Removal of the oxygen inhibition layer with a scaler increased cell viability but also resulted in higher surface roughness values. Conclusions HGF cell viability is affected by the surface treatment and the curing mode. The oxygen inhibition layer is an inhibitory factor for the viability of HGF cells. Autopolymerization enhances the cytotoxic potential of the oxygen inhibition layer.

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Influence of Material and Surface Roughness of Resin Composite Cements on Fibroblast Behavior

Abstract: Clinical Relevance A well-polished cement surface increases the viability and spreading of gingival fibroblasts. The tested resin composite cements did not reveal any cytotoxic effects.

Cited by 15 publications

References 36 publications

Fibroblast behavior on conventionally processed, milled, and printed occlusal device materials with different surface treatments

Fibroblast behavior on conventionally processed, milled, and printed occlusal device materials with different surface treatments

Influence of zirconia implant surface topography on first bone implant contact within a prospective cohort study

Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior

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