Influence of menstrual cycle and oral contraceptive phases on bone (re)modelling markers in response to intervallic running

Abstract

ObjectivesTo examine procollagen type I N-propeptide (P1NP) and carboxy-terminal cross-linking telopeptide of type I collagen (β-CTX-1) concentrations across different phases of the menstrual (MC) and oral contraceptive (OC) cycles and in response to running.Methods17β-oestradiol, progesterone, P1NP and β-CTX-1 were analysed pre-and post-exercise in eight eumenorrheic females in the early-follicular (EFP), late-follicular (LFP), and mid-luteal (MLP) phases of the MC, while 8 OC users were evaluated during the withdrawal (WP) and active pill-taking (APP) phases. The running protocol consisted of 8x3 min treadmill runs at 85% of maximal aerobic speed.Results17β-oestradiol concentrations (pg·ml-1) were lower in the EFP (47.22±39.75) compared to the LFP (304.95±235.85; p=<0.001) and MLP (165.56±80.6; p=0.003) of the MC and higher in the WP (46.51±44.09) compared to the APP (10.88±11.24; p<0.001) of the OC cycle. While progesterone (ng·ml-1) was higher in the MLP (13.214±4.926) compared to the EFP (0.521±0.365; p<0.001) and LFP (1.677±2.586; p<0.001) of the MC. In eumenorrheic females, P1NP concentrations (ng·ml-1) were higher in LFP (69.97±17.84) compared to EFP (60.96±16.64; p=0.006;) and MLP (59.122±11.77; p=0.002;). Post-exercise concentrations (70.71±15.59) increased from pre-exercise (55.86±12.86; p<0.001). For β-CTX-1 (ng·ml-1), lower concentrations were shown in MLP (0.376±0.098) compared to LFP (0.496±0.166; p=0.001) and EFP (0.452±0.148; p=0.039). OC users showed higher post-exercise P1NP concentrations in WP (61.75±8.32) compared to post-exercise in APP (45.45±6; p<0.001). Comparing P1NP levels between eumenorrheic and OC users, post-exercise P1NP concentrations were higher in the EFP (66.91±16.26; p<0.001), LFP (80.66±16.35; p<0.001) and MLP (64.57±9.68; p=0.002) of the MC compared to the APP of the OC cycle.ConclusionThese findings underscore the importance of studying exercising females with different ovarian hormone profiles, as changes in sex hormone concentrations affect bone metabolism in response to running, showing a higher post-exercise P1NP concentrations in all MC phases compared with APP of the OC cycle.