Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces

Mucientes, Arkaitz; Herranz, Eva; Moro, Enrique; González-Corchón, Aranzazu; Peña-Soria, María Jesús; Abásolo, Lydia; Rodríguez‐Rodríguez, Luis; Lamas, José Ramón; Fernández‐Gutiérrez, Benjamín

doi:10.3390/cells10020481

Cited by 12 publications

(8 citation statements)

References 49 publications

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“…For example, although DPSCs and PLSCs share similar DNA methylation patterns, slight differences in 5-mC in skeletal-related genes (e.g., SMAD and CD109) confer variations in osteogenesis capacity (Ai et al, 2018). In this regard, DPSCs exhibit higher mineralization capacity than other MSCs, including BMSCs, ATSCs, and different DT-MSCs (Xu et al, 2013;Padial-Molina et al, 2019;Shen et al, 2019;Mucientes et al, 2021). Thus, not surprisingly, the hypomethylation of the WNT10B locus (Figure 1G) facilitates its expression in DPSCs (Fracaro et al, 2020) to favor osteogenesis over adipogenesis, which suggests that DNA methylation is a mechanism underlying adipocyte and bone cell development.…”

Section: Discussionmentioning

confidence: 99%

Higher Expression of DNA (de)methylation-Related Genes Reduces Adipogenicity in Dental Pulp Stem Cells

Argaez-Sosa

Rodas-Junco

Carrillo-Cocom

et al. 2022

Front. Cell Dev. Biol.

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Obesity is a significant health concern that has reached alarming proportions worldwide. The overconsumption of high-energy foods may cause metabolic dysfunction and promote the generation of new adipocytes by contributing to several obesity-related diseases. Such concerns demand a deeper understanding of the origin of adipocytes if we want to develop new therapeutic approaches. Recent findings indicate that adipocyte development is facilitated by tight epigenetic reprogramming, which is required to activate the gene program to change the fate of mesenchymal stem cells (MSCs) into mature adipocytes. Like adipose tissue, different tissues are also potential sources of adipocyte-generating MSCs, so it is interesting to explore whether the epigenetic mechanisms of adipogenic differentiation vary from one depot to another. To investigate how DNA methylation (an epigenetic mark that plays an essential role in controlling transcription and cellular differentiation) contributes to adipogenic potential, dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were analyzed during adipogenic differentiation in vitro. Here, we show that the capacity to differentiate from DPSCs or PLSCs to adipocytes may be associated with the expression pattern of DNA methylation-related genes acquired during the induction of the adipogenic program. Our study provides insights into the details of DNA methylation during the adipogenic determination of dental stem cells, which can be a starting point to identify the factors that affect the differentiation of these cells and provide new strategies to regulate differentiation and adipocyte expansion.

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Section: Discussionmentioning

confidence: 99%

Higher Expression of DNA (de)methylation-Related Genes Reduces Adipogenicity in Dental Pulp Stem Cells

Argaez-Sosa

Rodas-Junco

Carrillo-Cocom

et al. 2022

Front. Cell Dev. Biol.

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“…The MSCs markers were identified using flow cytometry, while Alizarin red was utilized for staining culture calcium deposition in tissues, whereas cell proliferation was performed according to the AlamarBlue assay. Finally, cell lysates were used to measure ALP activity after centrifugation, as illustrated in Figure 9 [153,154]. marker to identify osteogenic differentiation of bone marrow MSCs [65].…”

Section: Graphene Oxide In Rat Mesenchymal Stem Cellsmentioning

confidence: 99%

Impact of Graphene Derivatives as Artificial Extracellular Matrices on Mesenchymal Stem Cells

et al. 2022

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Thanks to stem cells’ capability to differentiate into multiple cell types, damaged human tissues and organs can be rapidly well-repaired. Therefore, their applicability in the emerging field of regenerative medicine can be further expanded, serving as a promising multifunctional tool for tissue engineering, treatments for various diseases, and other biomedical applications as well. However, the differentiation and survival of the stem cells into specific lineages is crucial to be exclusively controlled. In this frame, growth factors and chemical agents are utilized to stimulate and adjust proliferation and differentiation of the stem cells, although challenges related with degradation, side effects, and high cost should be overcome. Owing to their unique physicochemical and biological properties, graphene-based nanomaterials have been widely used as scaffolds to manipulate stem cell growth and differentiation potential. Herein, we provide the most recent research progress in mesenchymal stem cells (MSCs) growth, differentiation and function utilizing graphene derivatives as extracellular scaffolds. The interaction of graphene derivatives in human and rat MSCs has been also evaluated. Graphene-based nanomaterials are biocompatible, exhibiting a great potential applicability in stem-cell-mediated regenerative medicine as they may promote the behaviour control of the stem cells. Finally, the challenges, prospects and future trends in the field are discussed.

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“…This capacity can be enhanced by supplementing cultures with 17ßestradiol (37). When cultured on different surfaces such as plastic, hydroxyapatite and β-tricalcium phosphate, DPSCs show higher proliferation capacity and greatest osteogenic potential when compared to cells isolated from adipose tissue, and bone marrow (38).…”

Section: Dental Pulp Stem Cellsmentioning

confidence: 99%

Wnt Signalling in Regenerative Dentistry

Birjandi¹,

Sharpe²

2021

Front. Dent. Med

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Teeth are complex structures where a soft dental pulp tissue is enriched with nerves, vasculature and connective tissue and encased by the cushioning effect of dentin and the protection of a hard enamel in the crown and cementum in the root. Injuries such as trauma or caries can jeopardise these layers of protection and result in pulp exposure, inflammation and infection. Provision of most suitable materials for tooth repair upon injury has been the motivation of dentistry for many decades. Wnt signalling, an evolutionarily conserved pathway, plays key roles during pre- and post-natal development of many organs including the tooth. Mutations in the components of this pathway gives rise to various types of developmental tooth anomalies. Wnt signalling is also fundamental in the response of odontoblasts to injury and repair processes. The complexity of tooth structure has resulted in diverse studies looking at specific compartments or cell types of this organ. This review looks at the current advances in the field of tooth development and regeneration. The objective of the present review is to provide an updated vision on dental biomaterials research, focusing on their biological properties and interactions to act as evidence for their potential use in vital pulp treatment procedures. We discuss the outstanding questions and future directions to make this knowledge more translatable to the clinics.

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Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces

Cited by 12 publications

References 49 publications

Higher Expression of DNA (de)methylation-Related Genes Reduces Adipogenicity in Dental Pulp Stem Cells

Higher Expression of DNA (de)methylation-Related Genes Reduces Adipogenicity in Dental Pulp Stem Cells

Impact of Graphene Derivatives as Artificial Extracellular Matrices on Mesenchymal Stem Cells

Wnt Signalling in Regenerative Dentistry

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