Influence of metabolic and physical factors on production of diacetoxyscirpenol by Fusarium sambucinum Fuckel

Abstract

Fusarium sambucinum Fuckel 8099-1 was grown on Czapek-Dox peptone-supplemented medium at 15°C for 14 days, and the cultures were investigated for diacetoxyscirpenol (DAS) production by liquid-liquid extraction and gas chromatography. The addition of 150 mg of sorbic acid, a tricarboxylic acid cycle inhibitor, per liter stimulated both fungal growth and DAS production. Among the 13-hydroxy-13-methylglutaryl coenzyme A precursors tested, isovaleric acid completely inhibited fungal growth and DAS production, ethyl isovalerate did not support a significant increase in DAS production, and L-leucine partially inhibited DAS production, showing that L-leucine and isovaleric acid catabolisms do not induce trichothecene biosynthesis. Solid particles (cork powder) were necessary for DAS production in stationary cultures but did not influence DAS production in shaken cultures. Shaking strongly stimulated DAS production and fungal growth.

Mining the Volatilomes of Plant-Associated Microbiota for New Biocontrol Solutions

“…However, DMDS poorly performed in our assays, with the exception of zoospore activity. The inhibition of P. infestans radial growth and sporangia germination by isovaleric acid is especially interesting as this compound was shown to trigger the germination of Agaricus bisporus spores (Rast and Stauble, 1970 ) but not of ectomycorrhizal fungi (Fries, 1978 ), and to inhibit Fusarium growth (Monnet et al, 1988 ). Most other free fatty acids are considered as broad-range fungal inhibitors interfering with membrane composition (Pohl et al, 2011 ).…”

Σύνθεση Και Βιολογική Έρευνα Χημειοθεραπευτικών Ελεγχόμενης Απόδοσης Για Τον Έλεγχο Των Μυκοτοξικώσεων Και Μυκητιάσεων

“…Ο Monnet [Monnet, 1988] αναφέρει αναστολή της ανάπτυξης του Fusarium sambucinum και μείωση της παραγωγής της μυκοτοξίνης diacetoxyscirpenol από τον παραπάνω μύκητα για συγκεντρώσεις σορβικού οξέος άνω των 300 ppm. Ο Tong [Tong, 1985] αναφέρει πλήρη αναστολή στην ανάπτυξη του Αspergillus sulphureus και Penicillium viridicatum αλλά και της παραγωγής οχρατοξίνης Α από τα συγκεκριμένα είδη στη συγκέντρωση των 0.02% σορβικού καλίου και 0.2% προπιονικού νατρίου σε pH=4.5.…”