As the amount of fossil fuels is decreasing rapidly, we need to rely on latest technologies to conserve fossil fuels and discover alternative sources of energy. One of the alternative source of renewable energy is 'Biodiesel'. It is defined as monoalkyl ester derivatives of long chain fatty acids which can be used as an alternative to diesel. Thus, biodiesel was synthesized from sesame oil and mustard oil by trans-esterification process respectively. These two biodiesels were blended (B50). Oxidation Stability (OS) of biodiesel tells about the quality of synthesized biodiesel and it was checked in professional biodiesel Rancimat 893 according to IS 15607. Biodiesel synthesized from Sesame and Mustard oil has showed an effective OS in blended form (B50). The OS of Sesame and Mustard Methyl Ester(SSME) has come to be 5.48 hours respectively. So, this research paper is the Experimental Study on the Synthesis and Oxidation Stability Characteristics of Sesame and Mustard Methyl Ester in blended form (B50) Synthesized from Sesame and Mustard Oil.