Influence of mevinolin on chloroplast terpenoids in Cannabis sativa

Abstract

Plants synthesize a myriad of isoprenoid products that are required both for essential constitutive processes and for adaptive responses to the environment. Two independent pathways for the biosynthesis of isoprenoid precursors coexist within the plant cell: the cytosolic mevalonic acid (MVA) pathway and the plastidial methylerythritol phosphate (MEP) pathway. In this study, we investigated the inhibitory effect of the MVA pathway on isoprenoid biosynthesized by the MEP pathway in Cannabis sativa by treatment with mevinolin. The amount of chlorophyll a, b, and total showed to be significantly enhanced in treated plants in comparison with control plants. Also, mevinolin induced the accumulation of carotenoids and α-tocopherol in treated plants. Mevinolin caused a significant decrease in tetrahydrocannabinol (THC) content. This result show that the inhibition of the MVA pathway stimulates MEP pathway but none for all metabolites.

A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum

“…This conclusion is consistent with the biochemical properties of GFDPS, which was found to use GGDP as its preferred allylic cosubstrate; GGDP is also formed in plastids (Hemmerlin et al, 2012). The increase in sesterterpenoid accumulation upon MEV treatment has a precedent in previous studies that have shown how blocking one terpenoid biosynthetic pathway stimulates increases in terpenoid levels by the other pathway (Laule et al, 2003;Ramak et al, 2013;Mansouri and Salari, 2014). Despite the formation of L. canum sesterterpenoids from MEP pathway substrates, the GT transcriptome of this species contains sequences for nearly all steps of both the MEP and MVA pathways.…”

Closing the Yield Gap for Cannabis: A Meta-Analysis of Factors Determining Cannabis Yield

“…Several studies, cataloged in Table 1, have tested the role of biotic and abiotic elicitors on the effects of cannabinoid biosynthesis, revealing the sensitivity of this pathway to external signals. In contrast, studies conducted by Mansouri et al (2009a,b, 2011, 2013 and 2016), Mansouri and Asrar (2012), Mansouri and Rohani (2014) and Mansouri and Salari (2014) demonstrated that abscisic acid, cycocel, ethephon, gibberellic acid, and mevinolin can all alter cannabinoid biosynthesis. While Flores-Sanchez et al (2009) tested a large number of elicitors for effects on cannabis hairy root cell cultures, this did not induce cannabinoid biosynthesis.…”

Cannabis Microbiome and the Role of Endophytes in Modulating the Production of Secondary Metabolites: An Overview

“…The precise role of cannabinoid in plant defence is not yet known. The plant growth regulators, including ABA, cycocel, ethephon, GAs, salicylic acid, γ-Aminobutyric acid (GABA), and mevinolin can manipulate cannabinoid biosynthesis and modulating secondary cannabis metabolites [22,[118][119][120][121].…”