Influence of microneedle shape on the transport of a fluorescent dye into human skin in vivo

Bal, Suzanne M.; Kruithof, Annelieke C.; Zwier, Raphaël; Dietz, Ekkehart; Bouwstra, Joke A.; Lademann, Jürgen; Meinke, Martina C.

doi:10.1016/j.jconrel.2010.07.104

Cited by 74 publications

(53 citation statements)

References 28 publications

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“…4) with the model agent fluorescein which was applied either before or after piercing. 32 They observed that the shape of the conduits formed by microneedle piercing was dependent on the type of MA used and the microneedles with the shape of oblique section-cut cylinder (Fig. 4A, named 300A for convenience) formed half-moon shaped conduits which were much larger and deeper than those visualized after treatment with the other 2 MAs, which had shapes of oblique section-cut square column (Fig.…”

Section: Size and Geometry Of Mpmasmentioning

confidence: 98%

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Multifunctional particle-constituted microneedle arrays as cutaneous or mucosal vaccine adjuvant-delivery systems

Wang

et al. 2016

Human Vaccines & Immunotherapeutics

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To overcome drawbacks of current injection vaccines, such as causing needle phobia, needing health professionals for inoculation, and generating dangerous sharps wastes, researchers have designed novel vaccines that are combined with various microneedle arrays (MAs), in particular, with the multifunctional particle-constructed MAs (MPMAs). MPMAs prove able to enhance vaccine stability through incorporating vaccine ingredients in the carrier, and can be painlessly inoculated by minimally trained workers or by selfadministration, leaving behind no metal needle pollution while eliciting robust systemic and mucosal immunity to antigens, thanks to delivering vaccines to cutaneous or mucosal compartments enriched in professional antigen-presenting cells (APCs). Especially, MPMAs can be easily integrated with functional molecules fulfilling targeting vaccine delivery or controlling immune response toward a Th1 or Th2 pathway to generate desired immunity against pathogens. Herein, we introduce the latest research and development of various MPMAs which are a novel but promising vaccine adjuvant delivery system (VADS).

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Section: Size and Geometry Of Mpmasmentioning

confidence: 98%

“…32 Many relevant factors have been considered crucial to formulate an optimized microneedle array system, including the geometry and shape of the microneedles, microneedle density, microneedle length, and microneedle bottom and even tip radius.…”

Section: Size and Geometry Of Mpmasmentioning

confidence: 99%

Multifunctional particle-constituted microneedle arrays as cutaneous or mucosal vaccine adjuvant-delivery systems

Wang

et al. 2016

Human Vaccines & Immunotherapeutics

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“…As well as visualising the penetration of substances into the skin, the effect of encapsulation of a dye into particles on the penetration pathways can be studied (Ossadnik et al, 2006), and CLSM can be used to study morphological changes of the skin (Dietterle et al, 2008, Eichert et al, 2010, Meyer et al, 2007. Recently, we showed that by using CLSM it is possible to visualise the conduits made by solid microneedle treatment in human volunteers (Bal et al, 2010b) and to compare different microneedle systems (Bal et al, 2010c). One of these microneedle arrays was selected to compare the penetration of a fluorescent dye applied in solution with that applied in liposomes.…”

Section: A C Bmentioning

confidence: 99%

Application of in vivo Laser Scanning Microscopy to Visualise the Penetration of a Fluorescent Dye in Solution and in Liposomes into the Skin after Pre-Treatment with Microneedles

Martina¹,

Annelieke²,

Suzanne³

et al. 2011

Laser Scanning, Theory and Applications

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“…The delivery and transport of fluorescent molecules in the skin has been previously visualized (Bal et al, 2010;Chen et al, 2012;Grams et al, 2004;Prow et al, 2010;Raphael et al, 2010), although the diffusion coefficient was not quantitatively determined. This parameter is essential to describe the passive transport of a molecule in a material and is dependent on both the properties of the diffusing molecule and the material through which it diffuses.…”

Section: Introductionmentioning

confidence: 99%

Diffusion profile of macromolecules within and between human skin layers for (trans)dermal drug delivery

Römgens

Bader

Bouwstra

et al. 2015

Journal of the Mechanical Behavior of Biomedical Materials

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Diffusion profile of macromolecules within and between human skin layers for (trans)dermal drug delivery, Journal of the Mechanical Behavior of Biomedical Materials, http://dx.doi. org/10.1016/j.jmbbm.2015.06.019 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractDelivering a drug into and through the skin is of interest as the skin can act as an alternative drug administration route for oral delivery. The development of new delivery methods, such as microneedles, makes it possible to not only deliver small molecules into the skin, which are able to pass the outer layer of the skin in therapeutic amounts, but also macromolecules. To provide insight into the administration of these molecules into the skin, the aim of this study was to assess the transport of macromolecules within and between its various layers. The diffusion coefficients in the epidermis and several locations in the papillary and reticular dermis were determined for fluorescein dextran of 40 and 500 kDa using a combination of fluorescent recovery after photobleaching experiments and finite element analysis. The diffusion coefficient was significantly higher for 40 kDa than 500 kDa dextran, with median values of 23 and 9 µm 2 /s in the dermis, respectively. The values only marginally varied within and between the papillary and reticular dermis. For the 40 kDa dextran, the diffusion coefficient in the epidermis was twice as low as in the dermis layers. The adopted method may be used for other macromolecules, which are of interest for dermal and transdermal drug delivery. The knowledge about diffusion in the skin is useful to optimize (trans)dermal drug delivery systems to target specific layers or cells in the human skin.

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Influence of microneedle shape on the transport of a fluorescent dye into human skin in vivo

Cited by 74 publications

References 28 publications

Multifunctional particle-constituted microneedle arrays as cutaneous or mucosal vaccine adjuvant-delivery systems

Multifunctional particle-constituted microneedle arrays as cutaneous or mucosal vaccine adjuvant-delivery systems

Application of in vivo Laser Scanning Microscopy to Visualise the Penetration of a Fluorescent Dye in Solution and in Liposomes into the Skin after Pre-Treatment with Microneedles

Diffusion profile of macromolecules within and between human skin layers for (trans)dermal drug delivery

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