Influence of Mild Metabolic Acidosis on Cardiac Contractility and Isoprenaline Response in Isolated Ovine Myocardium

Schotola, Hanna; Sossalla, Samuel; Rajab, Taufiek Konrad; Toischer, Karl; Quintel, Michael; Bauer, Martin; Schmitto, Jan D.

doi:10.1111/j.1525-1594.2011.01390.x

Cited by 11 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, we showed that an acute mild metabolic pH shift to 7.20 did not influence cardiac contractility in the isolated trabeculae of nonfailing ovine hearts [24]. The results of the current study with end-stage human failing myocardium show a significant decrease in force amplitude under comparable conditions.…”

Section: Discussionsupporting

confidence: 53%

“…As previously described, the trabeculae were superfused with a HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffering solution, pH 7.40 (m M : NaCl 116, KCl 5, NaH 2 PO 4 2, MgCl 2 1.2, Na 2 SO 4 1.2, NaHCO 3 20, glucose 10, CaCl 2 initially at 0.25 and after with a stepwise increase of 2.0), at a temperature of 37°C and oxygenated with 100% O 2 [24]. Contractions were caused by electrical field stimulation (baseline, 1 Hz; 5 to 7 mV; STIM1, Scientific Instruments).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, to our knowledge, little is known regarding the contractile behavior in vitro of the human myocardium under mild metabolic acidotic conditions. We recently showed that a mild and thus clinically relevant metabolic acidosis (pH 7.20) had no significant influence on the cardiac contractility and isoproterenol response in isolated trabeculae of the nonfailing ovine myocardium [24]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mild metabolic acidosis impairs the β-adrenergic response in isolated human failing myocardium

Schotola¹,

Toischer²,

Popov

et al. 2012

Crit Care

Self Cite

View full text Add to dashboard Cite

IntroductionPronounced extracellular acidosis reduces both cardiac contractility and the β-adrenergic response. In the past, this was shown in some studies using animal models. However, few data exist regarding how the human end-stage failing myocardium, in which compensatory mechanisms are exhausted, reacts to acute mild metabolic acidosis. The aim of this study was to investigate the effect of mild metabolic acidosis on contractility and the β-adrenergic response of isolated trabeculae from human end-stage failing hearts.MethodsIntact isometrically twitching trabeculae isolated from patients with end-stage heart failure were exposed to mild metabolic acidosis (pH 7.20). Trabeculae were stimulated at increasing frequencies and finally exposed to increasing concentrations of isoproterenol (0 to 1 × 10-6 M).ResultsA mild metabolic acidosis caused a depression in twitch-force amplitude of 26% (12.1 ± 1.9 to 9.0 ± 1.5 mN/mm2; n = 12; P < 0.01) as compared with pH 7.40. Force-frequency relation measurements yielded no further significant differences of twitch force. At the maximal isoproterenol concentration, the force amplitude was comparable in each of the two groups (pH 7.40 versus pH 7.20). However, the half-maximal effective concentration (EC50) was significantly increased in the acidosis group, with an EC50 of 5.834 × 10-8 M (confidence interval (CI), 3.48 × 10-8 to 9.779 × 10-8; n = 9), compared with the control group, which had an EC50 of 1.056 × 10-8 M (CI, 2.626 × 10-9 to 4.243 × 10-8; n = 10; P < 0.05), indicating an impaired β-adrenergic force response.ConclusionsOur data show that mild metabolic acidosis reduces cardiac contractility and significantly impairs the β-adrenergic force response in human failing myocardium. Thus, our results could contribute to the still-controversial discussion about the therapy regimen of acidosis in patients with critical heart failure.

show abstract

Section: Discussionsupporting

confidence: 53%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mild metabolic acidosis impairs the β-adrenergic response in isolated human failing myocardium

Schotola¹,

Toischer²,

Popov

et al. 2012

Crit Care

Self Cite

View full text Add to dashboard Cite

show abstract

“…As discussed earlier, the force of isolated human cardiac trabeculae increases ~81–168% as the stimulation frequency is increased from 0.5 Hz to 2.5 Hz (Chaudhary, et al, 2004; Pieske, et al, 1996; Rossman, et al, 2004). Increasing stimulation frequency in sheep trabeculae from 0.5 Hz to 2 Hz resulted in a 139–171% increase in force production (Schotola, et al, 2011; Sossalla, et al, 2010). Although, further increasing stimulation frequency to 3 Hz resulted in force decline.…”

Section: Sheep Modelsmentioning

confidence: 99%

Small and large animal models in cardiac contraction research: Advantages and disadvantages

Milani‐Nejad

Janssen

2014

Pharmacology & Therapeutics

383

310

View full text Add to dashboard Cite

The mammalian heart is responsible for not only pumping blood throughout the body but also adjusting this pumping activity quickly depending upon sudden changes in the metabolic demands of the body. For the most part, the human heart is capable of performing its duties without complications; however, throughout many decades of use, at some point this system encounters problems. Research into the heart’s activities during healthy states and during adverse impacts that occur in disease states is necessary in order to strategize novel treatment options to ultimately prolong and improve patients’ lives. Animal models are an important aspect of cardiac research where a variety of cardiac processes and therapeutic targets can be studied. However, there are differences between the heart of a human being and an animal and depending on the specific animal, these differences can become more pronounced and in certain cases limiting. There is no ideal animal model available for cardiac research, the use of each animal model is accompanied with its own set of advantages and disadvantages. In this review, we will discuss these advantages and disadvantages of commonly used laboratory animals including mouse, rat, rabbit, canine, swine, and sheep. Since the goal of cardiac research is to enhance our understanding of human health and disease and help improve clinical outcomes, we will also discuss the role of human cardiac tissue in cardiac research. This review will focus on the cardiac ventricular contractile and relaxation kinetics of humans and animal models in order to illustrate these differences.

show abstract

“…Hanna Schotola et al. (92) of the University Hospital Goettingen, Goettingen, Germany investigated the influence of mild (and thus clinically relevant) acidosis on myocardial contractility and catecholamine response in explanted trabeculae of healthy ovine hearts. Two groups (group 1: pH = 7.40, n = 9 and group 2: pH = 7.20, n = 13) were studied, and force amplitudes were measured at frequencies between 30 and 180 beats per minute and increasing catecholamine concentrations (isoprenaline 0‐3 × 10(−6) mM).…”

Section: Cardiovascular Supportmentioning

confidence: 99%

Artificial Organs 2011: A Year in Review

Malchesky¹

2012

Artificial Organs

View full text Add to dashboard Cite

In this Editor's Review, articles published in 2011 are organized by category and briefly summarized. As the official journal of The International Federation for Artificial Organs, The International Faculty for Artificial Organs, and the International Society for Rotary Blood Pumps, Artificial Organs continues in the original mission of its founders "to foster communications in the field of artificial organs on an international level."Artificial Organs continues to publish developments and clinical applications of artificial organ technologies in this broad and expanding field of organ replacement, recovery, and regeneration from all over the world. We take this time also to express our gratitude to our authors for offering their work to this journal. We offer our very special thanks to our reviewers who give so generously of time and expertise to review, critique, and especially provide meaningful suggestions to the author's work whether eventually accepted or rejected. Without these excellent and dedicated reviewers, the quality expected from such a journal would not be possible. We also express our special thanks to our Publisher, Wiley-Blackwell, for their expert attention and support in the production and marketing of Artificial Organs. In this Editor's Review, that historically has been widely well-received by our readership, we aim to provide a brief reflection of the currently available worldwide knowledge that is intended to advance and better human life while providing insight for continued application of technologies and methods of organ replacement, recovery, and regeneration. We look forward to recording further advances in the coming years.

show abstract

Influence of Mild Metabolic Acidosis on Cardiac Contractility and Isoprenaline Response in Isolated Ovine Myocardium

Cited by 11 publications

References 53 publications

Mild metabolic acidosis impairs the β-adrenergic response in isolated human failing myocardium

Mild metabolic acidosis impairs the β-adrenergic response in isolated human failing myocardium

Small and large animal models in cardiac contraction research: Advantages and disadvantages

Artificial Organs 2011: A Year in Review

Contact Info

Product

Resources

About