Influence of modified alginate hydrogels on mesenchymal stem cells and olfactory bulb-derived glial cells cultures

Abstract

BACKGROUND: Great potential of cellular therapies has generated extensive research in the field of cells harvesting and culturing. Transplantation of cell cultures has been used in a variety of therapeutic programs but in many cases it appeared that biomaterial scaffold or sheath would enhance cells regenerative potential. OBJECTIVE: Hydrogels composed of different proportions sodium and calcium alginates, were undertaken to evaluate their influence on mesenchymal stem cells and olfactory bulb-derived glial cells cultures. Additionally, these biomaterials were also enriched with fibrin protein. METHODS:The structure of materials was visualized by means of scanning electron microscopy. After seeding with cellshydrogels were observed with inverted and fluorescence microscope. Cell's morphology, behavior and phenotype were analyzed in investigated materials by means of light, fluorescence and scanning electron microscopes. Also, viability assay was performed with Alamar Blue cytotoxic test. RESULTS: Our observations showed that basic alginate hydrogels had significant influence on both cell types. Materials maintained cells alive, which is desired attribute, however none of them kept cells in normal, flat form. Alginates with significant calcium component kept cells alive for longer period of culture. CONCLUSIONS: Addition of fibrin protein resulted in material's biocompatibility properties improvement, by creation of adhesion surface, which helps cells to keep proper morphology and behavior. Our findings suggest that addition of fibrin protein to alginate hydrogels improves them as cell carriers for regenerative medicine applications.

Characterization of Olfactory Ensheathing Glial Cells Cultured on Polyurethane/Polylactide Electrospun Nonwovens

“…Biological experiments were approved by the Local Ethics Committee. Glial cells derived from olfactory bulb were isolated using the modified procedure described earlier [48,55]. Olfactory bulbs dissected from three neonatal pups' brains were placed in Hank's balanced salt solution (HBSS).…”

Polyurethane–polylactide-based material doped with resveratrol decreases senescence and oxidative stress of adipose-derived mesenchymal stromal stem cell (ASCs)

“…1 Regenerative medicine and tissue engineering offer patients both stem-cell-based therapy as well as biomaterial scaffolds, which are crucial for efficient treatment. [2][3][4] However, it has been shown that aging negatively correlates with stem cell multipotency, which might seriously limit their regenerative potential. In parallel with organism aging, progenitor cells lose their self-renewal, metabolic activity, and differentiation potential.…”

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

“…Various cell therapies, such as the use of OSCs, have been tested in the last decade [19,[42][43][44][45][46]. Transplanted olfactory cells promoted tissue nerve regeneration by paracrine signaling and by creating a conductive environment [47]. Although these studies showed partial success, an insufficient cell purification can cause complications by co-transplantation of mucusproducing cells [22,48,49].…”