Influence of Molecular Characteristics of Chitosan on Properties of In situ Formed Scaffolds

Grigoriev, T. E.; Zagoskin, Yu. D.; Белоусов, С. И.; Vasilyev, A. V.; Бухарова, Т. Б.; Leonov, Georgy; Galitsyna, Elena V.; Goldshtein, D. V.; Чвалун, С. Н.; Aa, Kulakov; Pal'tsev, M A

doi:10.1007/s12668-017-0411-5

Cited by 8 publications

(6 citation statements)

References 14 publications

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“…We previously performed a similar study with an evaluation of the cytocompatibility of different chitosan forms with MSC. There was no significant difference between the cell viability on the different chitosan-based material forms [ 24 ]. So the use of a one-stock chitosan sample with different degrees of deacetylation for studying the mechanical and biological properties of chitosan-based materials was an essential feature of this work.…”

Section: Discussionmentioning

confidence: 99%

Influence of the Degree of Deacetylation of Chitosan and BMP-2 Concentration on Biocompatibility and Osteogenic Properties of BMP-2/PLA Granule-Loaded Chitosan/β-Glycerophosphate Hydrogels

et al. 2021

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Compositions based on chitosan/β-glycerophosphate hydrogels with highly porous polylactide granules can be used to obtain moldable bone graft materials that have osteoinductive and osteoconductive properties. To eliminate the influence of such characteristics as chain length, degree of purification, and molecular weight on a designed material, the one-stock chitosan sample was reacetylated to degrees of deacetylation (DD%) of 19.5, 39, 49, 55, and 56. A study of the chitosan/β-glycerophosphate hydrogel with chitosan of a reduced DD% showed that a low degree of deacetylation increased the MSCs (multipotent stromal cells) viability rate in vitro and reduced the leukocyte infiltration in subcutaneous implantation to Wistar rats in vivo. The addition of 12 wt% polylactide granules resulted in optimal composite mechanical and moldable properties, and increased the modulus of elasticity of the hydrogel-based material by approximately 100 times. Excessive filling of the material with PLA (polylactide) granules (more than 20%) led to material destruction at a ~10% strain. Osteoinductive and osteoconductive properties of the chitosan hydrogel-based material with reacetylated chitosan (39 DD%) and highly porous polylactide granules impregnated with BMP-2 (bone morphogenetic protein-2) have been demonstrated in models of orthotopic and ectopic bone formation. When implanted into a critical-size calvarial defect in rats, the optimal concentration of BMP-2 was 10 μg/mL: bone tissue areas filled the entire material’s thickness. Implantation of the material with 50 μg/mL BMP-2 was accompanied with excessive growth of bone tissue and material displacement beyond the defect. Significant osteoinductive and osteoconductive properties of the material with 10 μg/mL of BMP-2 were also shown in subcutaneous implantation.

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Section: Discussionmentioning

confidence: 99%

Influence of the Degree of Deacetylation of Chitosan and BMP-2 Concentration on Biocompatibility and Osteogenic Properties of BMP-2/PLA Granule-Loaded Chitosan/β-Glycerophosphate Hydrogels

et al. 2021

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“…Chitosan (ChitoClear 43000—HQG 10, Primex, Iceland) with an average molecular weight of 80 kDa 58 and silver nitrate (AgNO 3 , ACS Reagent, ≥99.0%, Honeywell Fluka, USA) were used for the preparation of precursor solutions. The chitosan/silver nitrate solutions of 1 wt% in a 2 wt% aqueous acetic acid with a ratio of components by weight 1/0.10, 1/0.21, 1/0.52 and 1/1.04 were obtained and kept in a dark place at a temperature of 60 °С for 5 days for the growth of silver nanoparticles.…”

Section: Methodsmentioning

confidence: 99%

Porous chitosan particles doped by in situ formed silver nanoparticles: Electrorheological response in silicon oil

Kuznetsov

Kovaleva

Volkov

et al. 2022

Polymers for Advanced Techs

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Effect of in situ formed silver nanoparticles doping on electrorheological response of highly porous chitosan particles in suspensions of polydimethylsiloxane (silicone oil) is considered. Silver nanoparticles are directly reduced by chitosan from solution. Highly porous chitosan particles with different silver content are fabricated by spraying from solution followed by freeze‐drying. A high and stable electrorheological response of suspensions in wide range of electric field strength is observed at a very low filler content of 1 wt%. The nature of the electrorheological effect is considered from the standpoint of dielectric spectroscopy. The activation energies of polarization processes are determined from the temperature dependences of the dielectric loss modulus. The study shows the opportunity to control the properties of stimuli‐responsive materials by changing the structure and physicochemical properties of the functional filler. This approach opens up new possibilities for creating materials with high performance and predetermined properties.

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“…Extra virgin olive oil (F.lli De Cecco di Filippo Fara San Martino, Italy) was used as the dispersion medium. Polymer microparticles were produced from bulk chitosan (ChitoClear 43000-HQG 10, Primex, Iceland) with M n = 80 kDa . A 1 wt % solution of chitosan in diluted acetic acid (1 wt %) was sprayed in liquid nitrogen and then thermostated at −24 °C for nitrogen evaporation.…”

Section: Methodsmentioning

confidence: 99%

“…Polymer microparticles were produced from bulk chitosan (ChitoClear 43000-HQG 10, Primex, Iceland) with M n = 80 kDa. 45 A 1 wt % solution of chitosan in diluted acetic acid (1 wt %) was sprayed in liquid nitrogen and then thermostated at −24 °C for nitrogen evaporation. Frozen particles were dried by freeze-drying Alpha 2-4 LDplus (Martin Christ, Germany) for 3 days until the solvent was completely removed.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Is Chitosan the Promising Candidate for Filler in Nature-Friendly Electrorheological Fluids?

Kuznetsov

Zagoskin

Bakirov

et al. 2021

ACS Sustainable Chem. Eng.

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In this study, an electrorheological effect of the suspensions containing porous chitosan particles in olive oil in a wide range of electric fields is reported. Porous chitosan particles were produced by freeze-drying. The structure of the filler was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray scattering. Electrorheological behavior of low-filled fluids (0.1, 0.2, 0.5, and 1.0 wt %) was studied in shear and oscillation modes. Rheological data were fitted by Bingham, Cho–Choi–Jhon, and Seo–Seo models. Conductivity measurements were carried out to characterize the electrophysical properties of studied fluids. Polarization processes were considered from the standpoint of the Cole–Cole equation. Natural biodegradable materials such as chitosan and olive oil were used as components. The fluids showed high response to electric field and stable cyclic operation in on/off mode at extremely low concentrations. The yield stress reaches about 100 Pa for a 1 wt % suspension under an electric field of just 1 kV/mm. The sedimentation stability of the samples dramatically increases when the percolation threshold is passed. The sedimentation ratio at 1 wt % of the filler content remains at the level of 90% after almost a month. Thus, suspensions were considered as an alternative to typical silicon oil-based electrorheological fluids.

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Influence of Molecular Characteristics of Chitosan on Properties of In situ Formed Scaffolds

Cited by 8 publications

References 14 publications

Influence of the Degree of Deacetylation of Chitosan and BMP-2 Concentration on Biocompatibility and Osteogenic Properties of BMP-2/PLA Granule-Loaded Chitosan/β-Glycerophosphate Hydrogels

Influence of the Degree of Deacetylation of Chitosan and BMP-2 Concentration on Biocompatibility and Osteogenic Properties of BMP-2/PLA Granule-Loaded Chitosan/β-Glycerophosphate Hydrogels

Porous chitosan particles doped by in situ formed silver nanoparticles: Electrorheological response in silicon oil

Is Chitosan the Promising Candidate for Filler in Nature-Friendly Electrorheological Fluids?

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