Influence of motility on the outcome of in vitro fertilization/intracytoplasmic sperm injection with fresh vs. frozen testicular sperm from men with obstructive azoospermia

Park, Yong-Seog; Lee, Sunhee; Song, Seung-Hun; Jun, Jin Hyun; Koong, Mi Kyoung; Seo, Ju Tae

doi:10.1016/s0015-0282(03)00798-2

Cited by 86 publications

(79 citation statements)

References 18 publications

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“…While much controversy of fertilization and pregnancy rate remains over the cryopreservation of testicular spermatozoa [25][26][27], these results indicate that there are no adverse effects on IVF outcomes [28][29][30]. Previously, Park et al [8] reported that t-TESE had a lower fertilization rate than fresh TESE, but they did not find significantly different ongoing pregnancy rates in OA. Recently, similar data showed that IVF-ICSI outcomes with t-TESE suggest that cryopreservation does not adversely affect this procedure [31].…”

Section: Discussionmentioning

confidence: 90%

“…The testicular sperm extraction and preparation procedure was performed as described previously [8,22]. Briefly, a small piece (1 cm 3 ) of extruded testicular tissue was excised and placed in Ham's F-10 medium (Sigma, St. Louis, MO) supplemented with 0.4 % (w/v) human serum albumin (HSA; Sigma).…”

Section: Testicular Sperm Preparation and Freezing And Thawingmentioning

confidence: 99%

“…testicular spermatozoa or tissue are reliable approaches for the management of azoospermic patients and could all for the possibility of multiple IVF-ICSI procedures [7]. Previously Park et al [8] reported that acceptable fertilization and pregnancy rates were achieved using frozen testicular spermatozoa as opposed to fresh testicular spermatozoa in obstructive azoospermia (OA). Although the use of cryopreserved testicular sperm is not easy because of their low numbers and motility [3,9], there were no differences in fertilization and pregnancy rates for fresh and thawed testicular sperm from men with OA and non-obstructive azoospermia (NOA) [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Park

Kim

Lim

et al. 2014

J Assist Reprod Genet

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Purpose To evaluate the viability of frozen embryos generated by intracytoplasmic sperm injection (ICSI) with frozen testicular spermatozoa. Methods A total of 68 fresh embryo transfer (ET) cycles and 85 subsequent frozen-thawed ET (FET) cycles were grouped according to the source of spermatozoa: fresh testicular spermatozoa (TESE) or frozen-thawed testicular spermatozoa (t-TESE). Results There were no significant differences in the age of female patients, number of oocytes, or fertilization rates in fresh ET cycles with TESE (TESE-fresh ET) versus t-TESE (t-TESE-fresh ET). The rate of embryo survival after thawing (95.7 % vs. 94.0 %) was similar in frozen ET cycles (FET) with TESE (TESE-FET) and with t-TESE (t-TESE-FET). While there were significant differences in the proportion of good quality embryos, no statistical differences were found in the pregnancy or clinical abortion rates between the two groups. Moreover, delivery rates were not significantly different. Conclusions Although the proportion of good quality embryos was affected by cryopreservation of testicular tissue, embryo survival rate was not. As well, subsequent pregnancy could be achieved successfully via t-TESE-FET cycles. Therefore, FET is not affected by the cryopreservation of testicular tissue, and avoids further oocyte retrieval and TESE procedures.

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Section: Discussionmentioning

confidence: 90%

Section: Testicular Sperm Preparation and Freezing And Thawingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Park

Kim

Lim

et al. 2014

J Assist Reprod Genet

Self Cite

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“…Motile spermatozoa, either from fresh or frozen TESE, are necessary for optimal fertilization and pregnancy outcomes. Frozen testicular spermatozoa further reduced the availability of motile spermatozoa [23]. Thus, most men with OA or NOA can be treated successfully with TESE-ICSI with the exception of some cases.…”

Section: Discussionmentioning

confidence: 99%

Live birth after SrCl2 oocyte activation in previous repeated failed or low fertilization rates after ICSI of frozen-thawed testicular spermatozoa: case report

Kim

Choi

Yang

et al. 2012

J Assist Reprod Genet

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Purpose To report a live birth resulting after strontium chloride (SrCl 2 ) oocyte activation in a couple with complete fertilization failure or low fertilization rates following intracytoplasmic sperm injection (ICSI) of frozen-thawed testicular spermatozoa. Methods The couple underwent ICSI of frozen-thawed testicular spermatozoa. After ICSI, the oocytes were artificially activated by SrCl 2 because the results of fertilization were not satisfactory in the previous cycles. The main outcome measures were fertilization, pregnancy, and birth. Results In the first and second cycles performed previously at another clinic, fertilization rates were 9.1 % and 0.0 %, respectively. In the third cycle, 31 metaphase II oocytes were retrieved. After sperm injection, all of the oocytes were stimulated using SrCl 2 for activation. Sixteen oocytes were fertilized (51.6 %), and a single embryo was transferred into the uterus on Day 3. A healthy girl weighing 2750 g was born at 40 weeks of gestation by caesarean section. Conclusions This result suggests that SrCl 2 could be useful for oocyte fertilization in case of repeated complete fertilization failure or low fertilization rates following ICSI of frozen-thawed testicular spermatozoa.

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“…• Most reliable and well established method 24 • High rate of successful future sperm use after advances in IVF and ICSI 30 49% fatherhood in cancer patients using their frozen sperm 29 (Rates likely to be higher now after IVF advances) Sperm cryopreservation…”

Section: Male Patientsmentioning

confidence: 99%

Anticancer chemotherapy in teenagers and young adults: managing long term side effects

Ahmad

Reinius

Hatcher

et al. 2016

BMJ

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Influence of motility on the outcome of in vitro fertilization/intracytoplasmic sperm injection with fresh vs. frozen testicular sperm from men with obstructive azoospermia

Cited by 86 publications

References 18 publications

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Live birth after SrCl2 oocyte activation in previous repeated failed or low fertilization rates after ICSI of frozen-thawed testicular spermatozoa: case report

Anticancer chemotherapy in teenagers and young adults: managing long term side effects

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