Influence of mycorrhization on physiological parameters of micropropagated Castanea sativa Mill. plants

Martins, Anabela; Casimiro, Adalcina; Pais, M. S.

doi:10.1007/s005720050176

Cited by 37 publications

(24 citation statements)

References 30 publications

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“…As already reported, micropropagated chestnut plants showed increased growth and photosynthetic rates following in vitro mycorrhization with P. tinctorius (Martins et al 1996(Martins et al , 1997. The higher phosphate content in these plants revealed by both NMR and chemical analysis is in agreement with our previous results.…”

Section: Resultssupporting

confidence: 93%

“…They suggest that at high irradiance there is a positive mycorrhizal growth response in plants grown without additional P. In contrast, a reduced or negative mycorrhizal growth response occurs under low irradiance conditions, although the extent of the infection was similar (Son and Smith 1988). Micropropagated chestnut plants showed increased growth and photosynthetic rates following in vitro mycorrhization with P. tinctorius (Martins et al 1996(Martins et al , 1997.…”

Section: Introductionmentioning

confidence: 99%

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A 31P nuclear magnetic resonance study of phosphate levels in roots of ectomycorrhizal and nonmycorrhizal plants of Castanea sativa Mill.

Martins¹,

Santos

et al. 1999

Trees

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31 P-Nuclear Magnetic Resonance (NMR) was used to assess phosphate distribution in ectomycorrhizal and nonmycorrhizal roots of Castanea sativa Mill. as well as in the mycorrhizal fungus Pisolithus tinctorius in order to gain insight into phosphate trafficking in these systems. The fungus P. tinctorius accumulated high levels of polyphosphates during the rapid phase of growth. Mycorrhizal and nonmycorrhizal roots accumulate orthophosphate. Only mycorrhizal roots presented polyphosphates. The content in polyphosphates increased along the 3 months of mycorrhiza formation. In mycorrhizal roots of plants cultured under axenic conditions, the orthophosphate pool decreased along the culture time. In nonmycorrhizal roots the decrease in the orthophosphate content was less pronounced. The level of orthophosphate in mycorrhizal roots was significantly lower than in nonmycorrhizal ones, which indicates that this system relies upon the fungal polyphosphates as a major source of phosphate. & k w d :

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

A 31P nuclear magnetic resonance study of phosphate levels in roots of ectomycorrhizal and nonmycorrhizal plants of Castanea sativa Mill.

Martins¹,

Santos

et al. 1999

Trees

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“…Mycorrhizal symbiosis is frequently associated with increased photosynthetic rates of mycorrhizal plants (Harley and Smith, 1983;Reid et al, 1983;Bougher et al, 1990;Dosskey et al, 1990;Rousseau and Reid, 1990;Guehl and Garbaye, 1990;Jones et al, 1990;Martins, 1992;Martins et al, 1997;Smith and Read, 1997). ECM may influence the assimilation capacity for CO 2 in two distinct forms: increased absorption of P and N in mycorrhizal plants influence the photosynthetic rates, as observed for forestry species when amended with P; the other resulting from enhanced flux of carbon compounds to the roots, promoted by mycorrhizal associations (Martins et al, 1997;1999). This hypothesis considers that the increased photosynthetic rates are related with the fungus necessity of carbon compounds and is named source-sink concept (Dosskey et al, 1990;1991) although this seems to be just one of mechanism involved in photosynthetic increment in mycorrhizal plants (Martins et al, 1997;1999).…”

Section: Effect Of Mycorrhiza Inoculation On Plant Growthmentioning

confidence: 99%

“…(Martins et al, 1996).The fungi tested differed in their capacity to form mycorrhizas with C. sativa plants in vitro. P. tinctorius showed the best capacity to colonize chestnut roots either from seedlings or from micropropagated plants (Martins et al, 1996(Martins et al, , 1997.…”

Section: Mycorrhization Of Micropropageted Plantsmentioning

confidence: 99%

In vitro Mycorrhization of Micropropagated Plants: Studies on Castanea sativa Mill

Martins

2008

Mycorrhizae: Sustainable Agriculture and Forestry

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Abstract:In vitro mycorrhization can be made by several axenic and nonaxenic techniques but criticism exists about their artificiality and inability to reproduce under natural conditions. However, artificial mycorrhization under controlled conditions can provide important information about the physiology of symbiosis. Micropropagated Castanea sativa plants were inoculated with the mycorrhizal fungus Pisolithus tinctorius after in vitro rooting. The mycorrhizal process was monitored at regular intervals in order to evaluate the mantle and hartig net formation, and the growth rates of mycorrhizal and nonmycorrhizal plants. Plant roots show fungal hyphae adhesion at the surface after 24 hours of mycorrhizal induction. After 20 days a mantle can be observed and a hartig net is forming although the morphology of the epidermal cells remains unaltered. At 30 days of root-fungus contact the hartig net is well developed and the epidermal cells are already enlarged. After 50 days of mycorrhizal induction, growth was higher for mycorrhizal plants than for nonmycorrhizal ones. The length of the major roots was lower in mycorrhizal plants after 40 days. Fresh and dry weights were higher in mycorrhizal plants after 30 days. The growth rates of chestnut mycorrhizal plants are in agreement with the morphological development of the mycorrhizal structures observed at each mycorrhizal time. The assessment of symbiotic establishment takes into account the formation of a mantle and a hartig net that were already developed at 30 days, when differences between fresh and dry weights of mycorrhizal and nonmycorrhizal plants can be quantified. In vitro conditions, mycorrhization influences plant physiology after 20 days of root-fungus contact, namely in terms of growth rates. Fresh and dry weights, heights, stem diameter and growth rates increased while major root growth rate decreased in mycorrhizal plants.

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“…Despite the poor knowledge of their biodiversity, ecology or sustainable management in those regions, mushroom harvesting is increasing, mainly due to their economic importance to local populations (Baptista et al, 2010). Among the ectomycorrhizal (ECM) fungi known for having positive effects on plant hosts, e.g., (Martins, 1997;Martins et al, 1997), Laccaria laccata, Hebeloma crustuliniforme, H. sinapizans and Paxillus involutus (Branzanti et al, 1999), a special dedication has been devoted to P. arhizus and P. involutus (Carocho et al, 2012;Reis et al, 2011;Reis et al, 2012). Our research group conducted a few studies on symbiotic associations using Pinus pinaster as the plant host (Carocho et al, 2012;Reis et al, 2011;Reis et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the chemical interactions in co-culture elements of Castanea sativa Miller mycorrhization

Carvalho

Ferreira

Barreira

et al. 2013

Industrial Crops and Products

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In the early steps of mycorrhizal associations an oxidative burst might occur through the rapid production of high amounts of reactive oxygen species in response to external stimuli, increasing the production of antioxidants in plant and/or mycelia. Herein, the effect of mycorrhizal association among Pisolithus arhizus or Paxillus involutus and Castanea sativa Miller (stems and roots) was studied for different co-culture periods, defined for a better comprehension of the chemical interactions in the early stages of mycorrhization (6, 24 and 72 h). Knowledge on the specificities of plant-host interaction provided information about the most suitable fungus to be included in the development of new management strategies, and the fungal species that induced the strongest response with increasing antioxidant activity (free radical scavenging activity, reducing power and lipid peroxidation inhibition) and production of antioxidant compounds (phenolics and tocopherols) and sugars. P. involutus seemed to be the most adequate fungus to mycorrhize with C. sativa. Considering bioactive compounds production, P. arhizus was more efficient since allowed an increase in the contents of sugars and tocopherols in all co-culture elements.

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Influence of mycorrhization on physiological parameters of micropropagated Castanea sativa Mill. plants

Cited by 37 publications

References 30 publications

A 31P nuclear magnetic resonance study of phosphate levels in roots of ectomycorrhizal and nonmycorrhizal plants of Castanea sativa Mill.

A 31P nuclear magnetic resonance study of phosphate levels in roots of ectomycorrhizal and nonmycorrhizal plants of Castanea sativa Mill.

In vitro Mycorrhization of Micropropagated Plants: Studies on Castanea sativa Mill

Evaluation of the chemical interactions in co-culture elements of Castanea sativa Miller mycorrhization

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